REV3 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202996.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013251
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4515nts
Open reading frame: 1 to 4515

ATGTCGAGGGAGTCGAACGACACAATACAGAGCGATACGGTTAGATCATCCTCTAAATCA
GACTATTTTAGAATCCAGCTAAATAATCAAGACTACTATATGTCAAAACCCACCTTTTTG
GACCCATCGCACGGTGAAAGCCTGCCCTTAAACCAGTTTTCACAGGTCCCAAACATCAGG
GTGTTTGGTGCTTTACCCACAGGGCATCAAGTATTGTGCCACGTCCATGGCATTCTACCT
TACATGTTCATCAAGTATGATGGACAAATAACTGACACGAGTACATTGAGACACCAAAGA
TGTGCCCAAGTTCATAAAACGCTGGAAGTAAAAATTAGGGCATCCTTTAAAAGGAAAAAA
GATGATAAACACGATTTAGCCGGCGACAAACTTGGAAATCTCAATTTTGTCGCTGATGTC
TCTGTTGTAAAGGGTATACCATTTTATGGTTACCACGTTGGCTGGAATCTGTTTTACAAA
ATTTCTTTGCTTAATCCTTCTTGTCTAAGTAGGATTTCGGAATTGATTAGAGATGGCAAG
ATTTTCGGAAAGAAATTCGAAATTTATGAATCACATATTCCTTACTTGCTACAATGGACC
GCAGATTTTAACTTATTTGGATGTTCATGGATAAACGTGGATAGGTGTTATTTTCGCTCT
CCTGTTTTAAATAGTATACTGGACATAGATAAGCTGACAATTAATGATGATCTTCAGTTG
TTACTAGATCGATTTTGTGACTTCAAGTGTAACGTACTCAGTAGAAGGGATTTTCCACGG
GTTGGAAATGGATTGATTGAGATAGATATACTACCGCAATTCATCAAAAATAGAGAAAAG
TTACAGCATAGAGATATACATCACGACTTTTTGGAAAAACTGGGAGATATTTCTGACATA
CCTGTCAAACCATATGTATCCTCTGCTAGGGACATGATAAATGAACTTACCATGCAACGA
GAGGAGTTGTCCTTGAAGGAATATAAAGAACCACCAGAAACAAAGCGCCATGTGAGTGGT
CATCAATGGCAATCTTCTGGAGAGTTCGAAGCGTTTTATAAGAAAGCACAACATAAAACA
AGTACTTTTGATGGTCAAATACCAAATTTTGAGAACTTTATAGATAAGAATCAAAAATTC
TCAGCCATAAATACGCCCTATGAAGCATTACCGCAATTGTGGCCAAGGCTTCCACAGATT
GAAATAAACAATAATAGTATGCAAGATAAAAAGAACGACGACCAAGTTAATGCATCGTTC
ACCGAATACGAGATATGTGGTGTTGATAATGAGAACGAAGGTGTAAAAGGAAGCAATATA
AAGTCTCGGTCTTATTCATGGTTACCGGAAAGCATTGCGTCTCCGAAAGATTCTACTATA
CTATTAGATCATCAAACAAAATATCACAATACGATAAATTTTTCAATGGATTGTGCTATG
ACGCAAAATATGGCAAGTAAACGAAAACTTAGATCATCTGTTTCTGCTAATAAAACATCG
TTGCTGTCTCGGAAAAGAAAGAAGGTTATGGCTGCGGGATTACGCTATGGAAAAAGAGCC
TTTGTTTATGGTGAGCCTCCTTTCGGTTATCAAGATATTCTGAATAAATTGGAAGACGAG
GGATTCCCAAAAATAGACTATAAGGACCCTTTCTTTTCGAATCCAGTTGATCTAGAAAAT
AAACCTTACGCGTACGCAGGTAAGAGGTTTGAAATAAGCTCGACACATGTATCAACGAGA
ATACCCGTCCAATTTGGGGGAGAGACCGTATCAGTTTATAACAAGCCAACTTTCGACATG
TTTTCCTCCTGGAAATATGCGTTAAAACCACCTACGTATGATGCTGTTCAAAAATGGTAC
AATAAAGTACCCTCGATGGGAAACAAAAAAACTGAGTCTCAGATAAGCATGCACACCCCT
CATAGTAAGTTTTTATACAAATTTGCTAGTGACGTTTCTGGAAAACAAAAAAGAAAAAAA
AGTAGCGTTCACGATTCTCTTACTCATCTGACACTTGAGATTCATGCAAACACGAGAAGT
GATAAAATTCCAGACCCTGCAATAGATGAAGTTTCCATGATTATATGGTGTCTTGAAGAA
GAAACTTTCCCTTTAGATTTGGATATCGCTTACGAAGGGATTATGATAGTCCACAAAGCT
TCTGAGGATAGTACATTTCCTACTAAAATTCAACATTGTATTAACGAAATTCCCGTGATG
TTCTATGAAAGTGAATTTGAAATGTTTGAGGCTCTAACTGATTTGGTATTACTTCTTGAT
CCTGATATTCTTTCCGGTTTCGAAATACATAATTTTTCGTGGGGTTATATAATTGAAAGG
TGTCAAAAAATACATCAATTTGACATTGTCAGGGAACTTGCCAGAGTCAAATGCCAAATT
AAGACGAAGTTGTCAGATACTTGGGGATATGCCCATTCCTCAGGAATTATGATTACCGGA
AGACATATGATTAATATATGGAGAGCGTTGAGGTCCGACGTAAATTTAACGCAATATACT
ATTGAAAGTGCTGCGTTCAATATCCTCCATAAACGATTACCTCACTTTTCATTTGAGTCA
TTAACTAATATGTGGAACGCTAAAAAGAGCACAACCGAATTAAAGACTGTGTTAAATTAT
TGGTTATCAAGAGCTCAGATAAATATACAACTATTAAGAAAGCAGGATTACATTGCTCGA
AATATCGAACAGGCAAGATTGATAGGAATAGATTTCCATTCTGTATATTACAGAGGATCA
CAATTTAAAGTTGAGTCTTTTTTAATCCGAATATGCAAGTCCGAAAGTTTTATCCTTCTT
TCTCCAGGCAAAAAGGATGTTCGTAAGCAAAAAGCACTTGAATGTGTGCCTTTGGTTATG
GAGCCAGAATCTGCTTTCTACAAAAGTCCTTTAATTGTGCTGGATTTCCAATCATTGTAT
CCATCCATTATGATTGGATACAACTATTGCTATTCGACTATGATAGGAAGAGTGCGAGAA
ATAAACTTAACGGAAAATAACCTTGGAGTATCTAAGTTTTCATTACCAAGAAACATTTTA
GCTTTATTAAAAAATGATGTAACTATCGCTCCAAATGGTGTTGTTTATGCCAAGACCTCT
GTTAGAAAATCAACGTTATCCAAAATGTTAACAGATATCCTTGATGTCAGAGTGATGATA
AAGAAAACAATGAACGAAATAGGTGATGACAACACTACCCTAAAAAGGCTTTTGAATAAC
AAACAGTTAGCACTAAAATTATTGGCGAATGTCACCTACGGTTATACATCAGCTTCATTT
TCTGGACGAATGCCATGCTCTGATTTAGCTGATAGCATTGTACAAACAGGCAGAGAAACA
TTGGAGAAAGCAATAGATATTATTGAGAAAGATGAAACTTGGAACGCCAAAGTTGTCTAT
GGAGACACAGATAGTTTATTTGTATATCTACCTGGAAAAACTGCTATTGAGGCTTTTTCT
ATAGGACATGCTATGGCAGAAAGAGTTACTCAAAACAATCCAAAACCAATCTTTTTGAAG
TTTGAAAAAGTATACCATCCCTCCATATTAATTAGCAAAAAAAGGTACGTAGGATTTTCC
TATGAAAGTCCTTCGCAGACCCTTCCTATTTTTGATGCTAAGGGTATTGAAACTGTTAGA
AGGGATGGTATCCCAGCCCAGCAGAAGATTATTGAAAAATGTATTCGATTACTTTTTCAA
ACCAAAGACCTGTCAAAAATAAAAAAGTATCTTCAAAATGAATTTTTTAAGATTCAAATA
GGAAAGGTATCTGCCCAAGATTTTTGTTTTGCAAAAGAAGTTAAATTAGGAGCGTATAAA
AGCGAAAAGACAGCCCCTGCAGGTGCCGTGGTTGTAAAAAGAAGAATAAATGAAGACCAT
AGAGCAGAACCCCAATACAAGGAGCGTATACCTTACCTTGTTGTTAAAGGAAAGCAAGGA
CAACTGCTTCGGGAAAGATGCGTATCACCAGAAGAGTTCTTAGAAGGTGAGAATTTAGAG
TTAGATTCGGAGTATTATATAAACAAAATTCTGATACCTCCTCTTGATAGATTGTTCAAT
TTGATTGGTATAAATGTTGGCAACTGGGCTCAGGAAATAGTAAAATCCAAAAGGGCGAGC
ACAACTACTACAAAAGTGGAAAACATAACAAGAGTAGGAACTTCTGCAACATGTTGTAAT
TGTGGTGAAGAATTGACTAAAATATGTTCACTTCAGTTATGTGATGACTGTTTAGAGAAA
AGAAGCACCACAACCTTATCATTTCTCATAAAGAAATTAAAGAGACAAAAAGAATACCAA
ACACTAAAGACCGTGTGCAGGACGTGCAGTTATCGTTACACTTCCGATGCAGGCATCGAA
AATGACCATATAGCTAGTAAATGCAATTCATATGACTGTCCAGTATTTTACTCTCGTGTC
AAAGCAGAAAGATACTTGAGAGATAATCAATCTGTTCAAAGGGAAGAAGCATTAATATCT
CTAAATGATTGGTAG

Protein sequence predicted based on nucleotide sequence

MSRESNDTIQSDTVRSSSKSDYFRIQLNNQDYYMSKPTFLDPSHGESLPLNQFSQVPNIR
VFGALPTGHQVLCHVHGILPYMFIKYDGQITDTSTLRHQRCAQVHKTLEVKIRASFKRKK
DDKHDLAGDKLGNLNFVADVSVVKGIPFYGYHVGWNLFYKISLLNPSCLSRISELIRDGK
IFGKKFEIYESHIPYLLQWTADFNLFGCSWINVDRCYFRSPVLNSILDIDKLTINDDLQL
LLDRFCDFKCNVLSRRDFPRVGNGLIEIDILPQFIKNREKLQHRDIHHDFLEKLGDISDI
PVKPYVSSARDMINELTMQREELSLKEYKEPPETKRHVSGHQWQSSGEFEAFYKKAQHKT
STFDGQIPNFENFIDKNQKFSAINTPYEALPQLWPRLPQIEINNNSMQDKKNDDQVNASF
TEYEICGVDNENEGVKGSNIKSRSYSWLPESIASPKDSTILLDHQTKYHNTINFSMDCAM
TQNMASKRKLRSSVSANKTSLLSRKRKKVMAAGLRYGKRAFVYGEPPFGYQDILNKLEDE
GFPKIDYKDPFFSNPVDLENKPYAYAGKRFEISSTHVSTRIPVQFGGETVSVYNKPTFDM
FSSWKYALKPPTYDAVQKWYNKVPSMGNKKTESQISMHTPHSKFLYKFASDVSGKQKRKK
SSVHDSLTHLTLEIHANTRSDKIPDPAIDEVSMIIWCLEEETFPLDLDIAYEGIMIVHKA
SEDSTFPTKIQHCINEIPVMFYESEFEMFEALTDLVLLLDPDILSGFEIHNFSWGYIIER
CQKIHQFDIVRELARVKCQIKTKLSDTWGYAHSSGIMITGRHMINIWRALRSDVNLTQYT
IESAAFNILHKRLPHFSFESLTNMWNAKKSTTELKTVLNYWLSRAQINIQLLRKQDYIAR
NIEQARLIGIDFHSVYYRGSQFKVESFLIRICKSESFILLSPGKKDVRKQKALECVPLVM
EPESAFYKSPLIVLDFQSLYPSIMIGYNYCYSTMIGRVREINLTENNLGVSKFSLPRNIL
ALLKNDVTIAPNGVVYAKTSVRKSTLSKMLTDILDVRVMIKKTMNEIGDDNTTLKRLLNN
KQLALKLLANVTYGYTSASFSGRMPCSDLADSIVQTGRETLEKAIDIIEKDETWNAKVVY
GDTDSLFVYLPGKTAIEAFSIGHAMAERVTQNNPKPIFLKFEKVYHPSILISKKRYVGFS
YESPSQTLPIFDAKGIETVRRDGIPAQQKIIEKCIRLLFQTKDLSKIKKYLQNEFFKIQI
GKVSAQDFCFAKEVKLGAYKSEKTAPAGAVVVKRRINEDHRAEPQYKERIPYLVVKGKQG
QLLRERCVSPEEFLEGENLELDSEYYINKILIPPLDRLFNLIGINVGNWAQEIVKSKRAS
TTTTKVENITRVGTSATCCNCGEELTKICSLQLCDDCLEKRSTTTLSFLIKKLKRQKEYQ
TLKTVCRTCSYRYTSDAGIENDHIASKCNSYDCPVFYSRVKAERYLRDNQSVQREEALIS
LNDW*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4515
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable