VTH2 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH203006.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00013256
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4650nts
Open reading frame: 1 to 4650

ATGGCATTGTTTCGTGCCCTGTATATTATTTGGGTATTCCTACTCATTCCACTGTCCAAT
GCAGAAGAATTCACCCCCAAGGTTACCAGAACTTTGTCAAGATATGTATTCGATATAGTA
AACTTTGATGATTCAAACACTTTGATCAGAGCAGAAGAAGACTCTGTCGAGATAAGTTTT
GATGCTGGAGAAAATTGGAAAACAATAGATGAGATTGAAGAGCCTATCGAGTCATTTGTT
GTTGATCCCTTCCGTGGGCATGATAGAGCTTTTGCTTTCGTGAAAACGGCACCAAAATTT
TACGTCACGGATGATCAAGGTAAATCGTGGAGGCCTTTGACTATACCCATTTCTGAGAAA
GCATCGAATTATTTTTGCGACGTAACTACTCACCCTATAAAAAAGAAGCATCTTATTATT
CGTTGTGATTTGTTGACAATAAAAAACTCAGGCTTAATGTATGTTGGAAGAGAGATTTAC
ACAACTAATGATGGAGTATCCTTTTCCCAAGTTAAACCTTCTTTTGGAAAAATTGATGGT
CATATTAGCACGGCACGCTGTGACTTTATTAAATCTAGTGAGGATTCTGATCTGGGCGGT
AATGATGCTTCGATACTCTGTCTTTTCCGAAACACTGAGTACATTGAAAGTACAGGCTCA
ACTATTGACAAGTCTGAATTGATTTTAAGTGCCGATGGAGGTGAAACATTCAAAGAATTA
GTCCAGTTCAAAGATAAGGTTGTTAGTCGATACGAGATACTTAAGCATCATGTGATCGTT
TTGACACAAGATGATATGTATAATGAAATGTCATCAACAAACATTTGGATATCCAATGAT
GTATCCACTTTTCAAGTGGCTCGTACACCTACTAAGATACGGCACGTTAATATGGGACAA
ATTCATGAAGATTCCATCGGAAGAATCGTTTTACCTGTATCTAGAGAAAGAGATGATGAA
GACAGCAACCAACCGGGAGCTGCTGAAGTGTTAATATCAGATTCTGAAGGACTAAAATTT
TTGCCTATAAATTGGATACCAAACAATCAGTTTGGATATATCAATGTAGCTTATCCAGGT
TTCTTAAAAGGAACATTTTTTGGTTCGTTTCATCCCTTCATTGAATATTCTGATAGAAAA
AGGAAATACAGCCGACAGAAAGTAAGAGAGGAAACTAAAGTATCTGTTGATAACGGTCTC
ACATGGACAAACTTGAAAGTTGTTGACCGGGAAAATGTAGATTTATTTGGTTGTGATGTC
ACTAAACCCGAGAGATGTTCGCTTCAGACTCATTTCTATGATCTAAGAAATTTAAATCCC
TCTGCCGGAATCATGATGATATCGGGTATTGTTGGCGACGGCAGTGCATACAATTGGAAA
GAAGAAAAAACTTTCATCTCCAGGGATAGTGGTTTAACATGGAGGTTAGTCCATAATTCC
ACTGGTTTATATACTACTGGTGATCTGGGAAATATTATCATGTATATTCCATATCGTTCG
AATGAAAATGGCGATGTGCCTTCTAAATTTTATTATTCCTTGGATCAAGGTAAGACATGG
GGTGAATATGATCTAATCATGCCTATTTATCCATACAGATTAGTCAGCACAATATCAGAT
GGATCTGGTTCAAAATTTATTCTAACAGGAACGTCCATTACAGAAGATCCAATATTCATT
ACATATTCAATTGATTTTTCAGCGGTATTTGATTATAAATCATGTGAAGAGGGTGATTTT
GAAGATTGGAATCTAGCAGATGGAAAATGTGTTAATGGCGCCAAATACAAGTACAGAAGG
AGGAAACAGGACGCCCAGTGTTTGGTAAAAAAAGCATTCAAAGATTTGAGTTTAGATGAA
ACGCCTTGTAACAGTTGTACTGGATCCGACTACGAATGTTCGTTTGAATTCGTTAGAGAT
GCGAAGGGTGACTGTATACCAGATTATAATCTGATTGCCCTTTCCGATATATGTGACAAA
TCGAAGGGTAAATCTGTGTTAGTAAAGCCACTACAATTAATCAAAGGTGATAAATGTAAA
ACACCTATGAAAATTGAATCCGTAGACATTCCGTGTGACGAAATTCCAAAGGAGGGTTCG
AGTGACAAGGAAATAGTGACTACTGAAAACAAATTCGACTTTGAAATTAAATTTTACCAA
TACTTCGATACAGTTGCCGACGAATCTTTGGTCATGCTGAATTCGATAGGAGACGCCTAT
ATATCCCACGATGGTGGACAAACGATAAAAAGGTTTGACACTGACGGCGAAAAAATTGTC
GAAATCGTGTTTAACCCATACTTCAATTCTTCAGCATATTTGTTTGGCTCTAAAGGTAAC
ATATTCTTAACACACGATAGAGGATACTCTTTCATGATAGCTAAATTACCTGAAGCCAGG
CAATTAGGCATGCCACTAGATTTTAGTGCTAAAGCACAGGATACTTTTATTTACTATGGT
GGTAAGAATTGTGAATCAATTTTAAGTCCAGAATGTCATGCAGTGGCATACCTCACCAAA
GATGGGGGTGAAACTTTTACAGAAATGCTTGATAATGCAATTCATTGTGAGTTTGCAGGA
ACACTATTCAAATATCCGTCAAATGACGATATGGTTATGTGCCAAGTAAAGGAAAAGTTT
TCGCAAACAAGAAGTTTAGTTTCTTCTACTGACTTTTTCCAAGATGATAGAAAAACTGTC
TTTGAAAACATTATCGGCTACTTGTCTACTGGTGGCTATATCATTGTTGCCGTACCTCAT
GAAGACAATGAATTGAGGGCGTATGTAACTAATGATGGTGCTGAGTTTACCGAGGCAAAA
TTCCCATATGATGAAGATATTGGTAAGCAAGACGCATTCACTATTTTAGGATCTGAGGAA
GGATCAATATTTTTACATTTAGCAACAAACTTGGAATCAGGACACGATTTCGGAAACCTT
TTGAAATCCAACTCGAATGGTACTTCTTTTGTCACCTTAGAGCATGCCGTTAATAGAAAC
ACATTCGGTTATGTGGACTTTGAAAAAGTTCAAGGTCTTGAAGGTATTATTATCACCAAC
ATCGTTTCGAATAGCGAAAAGGTTGGCGAGAACAAGGAAGACGAACAATTGAAGACGAAA
ATCACCTTCAATGACGGTTCAGATTGGAACTTTTTGAAGCCTCCAAAGAAGGATTCGGAA
GGGAAAAAGTTTCCCTGTGATTCTGTGTCATTAGATAAATGTTCTCTACATTTGCATGGT
TACACTGAACGTAAGGATATCAGGGATACATATTCCTCTGGCTCCGCGTTAGGAATGATG
TTTGGTGTCGGAAATGTTGGCGATAGGCTTCTACCATATGAGGAGTGTTCCACTTTTCTA
ACCACTGATGGAGGAGAGACGTGGACTGAGGTTAAAAAAGGCCCCCATCAATGGGAGTAC
GGTGATCATGGTGGAGTCTTGGTTTTGGTCCCCGAAAACGCAGAAACTGATTCTATTTCT
TACTCCACCGATTTTGGTAAAACATGGAAAGATTATAAATTCTGTGGCGACAAAGTCCTC
GTAAAGGATATAATCACCGTTCCCAGAGATTCTGCTTTGAGGTTTTTGCTATTTGGAGAA
GCAAAAAACATGGGTAGTGGTTCATTCAGAACGTATACAATTGATTTTAGAAACATCTTC
GAGAGGCAATGTGAGTTTGATATTACCGGTAGAAAAAGGGCAGATTTTAAGTACTCTCCT
CTGGGTTCCAGAACTGGTTGTTTGTTTGGCCATAAAACAGAATTTTTACGTAAAACCGAT
GAAAAATGTTTTATTGGGAATATTCCACTTTCTGAATTTTCGAGGAACGTCAAAAACTGT
CCATGTACAAGACAAGATTTCGAATGTGACTATAATTTTTATAAAGCCAGTGATGGTACT
TGTAAATTAGTCAAAGGCTTAAGTTCGGCGAATGGTGCCGATATCTGCAAAAAGGAACCC
GACTTAATTGAATACTATGATTCCTCCGGCTATCGGAAAATTCCCTTATCAACCTGTAAA
GGTGGGCTGAAATTGGATGCACATTTAGCACCACATCCTTGTCCGGGAAAAGAAAAGGCA
TTCAGGGAAAAATACTCTATAAACACCGGCGCTTATGCGCTGGTTTTTGTTACAATTCTT
CTCGTTATTTTCTTTGTCGCATGGTTTGTATACGATAGAGGTATCAGGAGAAATGGGGGA
TTTTCAAGATTTGAAGAAATCAGATTAGGAGACGATGGGCTGATAGAAAACAATAGGACC
GATAGAGTTGTCAACATCATTGTAAGACTAGGATTATGCATTTCTTTAATCACCAAGTCT
GCGTTTCAACGCGCGAAGGCAGGTACAGCGCAACTTTCTTCAAAATTTAGGGCAAGGTTT
GGCAACAAAAAAGGTGCCACTTACTCTTCGCTGCTTCATGATCAGCTTTCAGATGAACCA
GATGGCTTCCATGAAGATTCCAACGATTTATCCAGTTTCAGAGGTCAAGGTAGTAATTCT
GAAATTGAGCAAGAAGATGTTGACACATCTCAACAAGAGCATACGTTGCGGACAGACCTA
CTTGGTGCTAGCAATATTCCAGACGCTTTGCCAGCGCGTAGTGCTTCTCACGAGTCCGAT
TTAGCAGCTGCACGCAGCGAAGACAAGTAG

Protein sequence predicted based on nucleotide sequence

MALFRALYIIWVFLLIPLSNAEEFTPKVTRTLSRYVFDIVNFDDSNTLIRAEEDSVEISF
DAGENWKTIDEIEEPIESFVVDPFRGHDRAFAFVKTAPKFYVTDDQGKSWRPLTIPISEK
ASNYFCDVTTHPIKKKHLIIRCDLLTIKNSGLMYVGREIYTTNDGVSFSQVKPSFGKIDG
HISTARCDFIKSSEDSDLGGNDASILCLFRNTEYIESTGSTIDKSELILSADGGETFKEL
VQFKDKVVSRYEILKHHVIVLTQDDMYNEMSSTNIWISNDVSTFQVARTPTKIRHVNMGQ
IHEDSIGRIVLPVSRERDDEDSNQPGAAEVLISDSEGLKFLPINWIPNNQFGYINVAYPG
FLKGTFFGSFHPFIEYSDRKRKYSRQKVREETKVSVDNGLTWTNLKVVDRENVDLFGCDV
TKPERCSLQTHFYDLRNLNPSAGIMMISGIVGDGSAYNWKEEKTFISRDSGLTWRLVHNS
TGLYTTGDLGNIIMYIPYRSNENGDVPSKFYYSLDQGKTWGEYDLIMPIYPYRLVSTISD
GSGSKFILTGTSITEDPIFITYSIDFSAVFDYKSCEEGDFEDWNLADGKCVNGAKYKYRR
RKQDAQCLVKKAFKDLSLDETPCNSCTGSDYECSFEFVRDAKGDCIPDYNLIALSDICDK
SKGKSVLVKPLQLIKGDKCKTPMKIESVDIPCDEIPKEGSSDKEIVTTENKFDFEIKFYQ
YFDTVADESLVMLNSIGDAYISHDGGQTIKRFDTDGEKIVEIVFNPYFNSSAYLFGSKGN
IFLTHDRGYSFMIAKLPEARQLGMPLDFSAKAQDTFIYYGGKNCESILSPECHAVAYLTK
DGGETFTEMLDNAIHCEFAGTLFKYPSNDDMVMCQVKEKFSQTRSLVSSTDFFQDDRKTV
FENIIGYLSTGGYIIVAVPHEDNELRAYVTNDGAEFTEAKFPYDEDIGKQDAFTILGSEE
GSIFLHLATNLESGHDFGNLLKSNSNGTSFVTLEHAVNRNTFGYVDFEKVQGLEGIIITN
IVSNSEKVGENKEDEQLKTKITFNDGSDWNFLKPPKKDSEGKKFPCDSVSLDKCSLHLHG
YTERKDIRDTYSSGSALGMMFGVGNVGDRLLPYEECSTFLTTDGGETWTEVKKGPHQWEY
GDHGGVLVLVPENAETDSISYSTDFGKTWKDYKFCGDKVLVKDIITVPRDSALRFLLFGE
AKNMGSGSFRTYTIDFRNIFERQCEFDITGRKRADFKYSPLGSRTGCLFGHKTEFLRKTD
EKCFIGNIPLSEFSRNVKNCPCTRQDFECDYNFYKASDGTCKLVKGLSSANGADICKKEP
DLIEYYDSSGYRKIPLSTCKGGLKLDAHLAPHPCPGKEKAFREKYSINTGAYALVFVTIL
LVIFFVAWFVYDRGIRRNGGFSRFEEIRLGDDGLIENNRTDRVVNIIVRLGLCISLITKS
AFQRAKAGTAQLSSKFRARFGNKKGATYSSLLHDQLSDEPDGFHEDSNDLSSFRGQGSNS
EIEQEDVDTSQQEHTLRTDLLGASNIPDALPARSASHESDLAAARSEDK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4650
Mutations:
None
Discrepancy:
c4547t,S1516L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable