BUD3 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH203013.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013259
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4911nts
Open reading frame: 1 to 4911

ATGGAGAAAGACCTGTCGTCTCTTTACTCTGAAAAGAAAGACAAAGAGAACGATGAAACC
TTATTTAACATCAAACTATCCAAATCTGTTGTCGAGACCACACCGCTAAATGGTCATTCA
TTGTTTGATGATGATAAATCACTTTCAGACTGGACGGATAATGTGTTCACTCAATCAGTA
TTCTATCACGGGTCAGATGACTTGATATGGGGGAAGTTCTTTGTCTGCGTGTACAAGTCC
CCCAACAGCAATAAGTTGAACGCTATAATATTCGACAAATTAGGAACATCATGCTTCGAA
TCCGTCGATATATCTTCCAACTCGCAATACTATCCGGCCATTGAGAATTTGAGTCCAAGT
GATCAGGAAAGCAATGTTAAGAAATGCATTGCTGTCATTCTGTTACAGCGCTATCCATTA
CTTTCACCATCAGACTTATCACAAATATTGTCCAATAAATCGGAAAATTGCGACTATGAC
CCCCCTTATGCTGGAGATTTGGCTAGTAGTTGCCAGTTGATAACAGCAGTTCCTCCAGAA
GATCTGGGGAAGCGCTTCTTTACATCAGGACTTCTGCAAAATAGATTTGTCAGCTCTACC
CTGTTAGATGTTATTTATGAAAACAATGAATCCACCATCGAACTAAATAATAGGTTGGTA
TTCCATCTGGGTGAACAACTTGAACAACTTTTTAACCCAGTCACAGAATACTCACCGGAA
CAGACAGAATATGGTTATAAGGCGCCAGAGGACGAATTACCCACAGAATCGGATGATGAT
CTTGTCAAGGCCATTTGCAACGAGTTATTACAACTACAAACAAATTTTACTTTCAATTTG
GTAGAATTTTTGCAAAAATTCCTGATCGCCTTGAGAGTCAGAGTACTCAATGAAGAAATT
AATGGGTTATCCACAACCAAATTAAATCGACTCTTCCCACCTACAATAGATGAAGTCACA
AGAATCAATTGTATTTTTCTAGACTCGCTAAAGACAGCAATCCCTTACGGTTCCCTCGAA
GTACTGAAGGCATGCAGCATTACTATTCCTTATTTCTACAAAGCATATACAAGACACGAG
GCGGCCACAAAGAACTTCAGCAAAGATATTAAATTGTTTATTAGGCATTTCAGCAATGTA
ATTCCAGAAAGAGAGGTCTACACGGAAATGAAAATCGAGAGTATAATTAAGGGACCTCAG
GAAAAACTACTGAAGCTAAAGTTAATTATAGAGAGATTGTGGAAGTCGAAAAAATGGAGA
CCGAAAAATCAAGAAATGGCAAAAAAATGCTACAACAATATCATTGATGTCATTGATTCG
TTTGGAAAATTAGATTCCCCACTTCATTCTTATAGTACCAGAGTATTTACTCCATCGGGA
AAAATCCTTACAGAATTAGCCAAATGCTGGCCCGTAGAACTGCAATACAAATGGCTGAAG
AGAAGGGTAGTCGGTGTGTATGATGTAGTGGATTTGAATGATGAAAATAAGAGAAATTTA
TTAGTCATATTCAGTGATTATGTGGTTTTCATCAATATACTGGAGGCAGAAAGTTACTAC
ACTTCAGATGGATCAAACAGGCCCTTAATCTCAGATATTTTAATGAACTCATTGATCAAC
GAAGTTCCGTTGCCCTCCAAGATCCCTAAGTTGAAAGTGGAGCGTCATTGCTATATAGAT
GAGGTTCTAGTTTCTATATTAGACAAAAGCACTCTACGTTTTGATCGATTGAAGGGAAAA
GATTCTTTCTCAATGGTATGTAAATTATCCTCTGCATTTATCTCTTCTTCGTCAGTTGCT
GACTTGATTACGAAGGCTAGAATTTTGGAAAAAGACACTGCATTTCATTTATTTAAAGCT
AGTAGAAGCCATTTTACATTATATTCTACTGCTCACGAGCTTTGCGCTTATGATTCCGAA
AAAATAAAATCAAAATTTGCCTTATTCCTGAACATACCACCATCCAAGGAGATATTGGAG
GTCAACAACCTTCATTTGGCTTTTTTTGCAAGATTTTGCAGTAACGATGGTAGAGATAAC
ATCGTAATCTTAGACGTCTTAACCAAACATGACGATAAACATATAGAAGTTACATCCGAT
AACATTGTTTTCACCATAATTAATCAATTGGCCATTGAAATACCGATATGCTTTTCTTCC
TTAAACTCATCGATGGCCAAAGATTTACTCTGTGTAAATGAGAATTTGATAAAAAACTTA
GAACATCAATTGGAAGAGGTCAAGCACCCTTCAACAGACGAACATAGGGCTGTTAATAGC
AAACTTTCCGGTGCATCCGATTTCGATGCTACTCACGAGAAGAAAAGATCATACGGTACC
ATAACAACATTTAGAAGCTATACAAGCGACTTGAAGGACAGTCCATCAGGCGATAATAGT
AATGTCACCAAGGAAACTAAGGAAATTTTACCAGTGAAACCTACGAAAAAGTCTTCAAAA
AAACCAAGAGAAATTCAAAAGAAGACCAAGACAAACGCCTCTAAAGCAGAGCACATAGAA
AAGAAGAAGCCTAACAAAGGCAAAGGGTTTTTTGGCGTGTTAAAAAATGTTTTTGGAAGT
AAAAGCAAGAGCAAGCCTTCACCAGTTCAAAGAGTGCCTAAAAAAATATCGCAGAGGCAT
CCTAAGTCTCCAGTGAAGAAGCCAATGACCTCAGAAAAGAAATCCTCCCCTAAAAGGGCA
GTCGTTTCATCTCCCAAAATTAAAAAGAAAAGTACTTCTTTTTCCACAAAAGAATCACAA
ACTGCTAAATCTTCTCTTCGAGCAGTTGAATTCAAATCTGATGACTTGATCGGAAAACCA
CCTGATGTTGGAAATGGCGCACATCCTCAAGAAAATACCAGAATATCTTCAGTAGTAAGG
GATACAAAATATGTCTCCTACAATCCCTCTCAGCCTGTGACAGAAAATACCAGTAACGAA
AAAAATGTCGAACCAAAAGCGGATCAATCCACAAAGCAGGATAACATTTCCAATTTTGCA
GATGTAGAGGTATCTGCGTCTTCTTATCCTGAAAAACTTGATGCAGAAACAGATGATCAA
ATAATTGGGAAGGCGACGAATTCGTCATCAGTTCATGGAAATAAAGAGCTGCCAGACCTT
GCTGAGGTGACTACAGCAAATAGGGTTTCTACAACATCGGCTGGGGACCAACGTATTGAT
ACCCAAAGCGAATTTTTACGTGCAGCTGATGTTGAAAACTTAAGTGATGACGATGAACAC
AGACAGAATGAAAGTAGAGTTTTTAACGATGACCTCTTTGGTGATTTTATTCCTAAGCAT
TACCGTAATAAACAGGAGAACATTAACAGCTCGAGTAATTTGTTTCCAGAGGGAAAGGTG
CCCCAAGAAAAGGGCGTATCAAATGAAAACACTAACATATCTCTCAAAACTAATGAAGAT
GCATCTACATTGACGCAGAAACTCTCTCCACAAGCGAGTAAAGTGCTGACAGAAAATTCT
AATGAATTAAAAGATACCAACAATGAAGGGAAGGACGCAAAGGACATAAAATTAGGAGAT
GATTACAGTGATAAAGAAACAGCGAAAGAAATAACTAAACCAAAAAATTTTGTTGAAGGA
ATAACTGAACGGAAAGAAATATTCCCCACTATTCCTAGGTTAGCGCCGCCAGCTTCAAAA
ATTAACTTTCAAAGGTCACCATCCTATATTGAGCTCTTTCAAGGAATGAGGGTGGTTTTA
GATAAGCATGATGCCCATTATAACTGGAAACGCTTGGCTAGTCAAGTCTCCTTAAGTGAG
GGACTAAAAGTCAATACTGAGGAAGATGCGGCAATTATAAATAAAAGTCAGGATGATGCC
AAGGCGGAAAGAATGACTCAAATTTCTGAAGTGATTGAGTATGAAATGCAGCAACCTATC
CCAACTTATTTGCCTAAGGCGCATCTAGATGACTCGGGTATTGAAAAAAGTGATGACAAA
TTCTTCGAAATTGAAGAAGAACTTAAGGAAGAATTGAAGGGCAGCAAAACCGGTAATGAA
GATGTCGGTAATAATAATCCATCCAATTCTATTCCAAAAATCGAGAAGCCCCCAGCATTC
AAAGTTATTAGAACATCGCCTGTGAGAATTATCGGAAGGACTTTTGAAGACACTAGAAAA
TATGAAAATGGCTCTCCATCTGATATTTCGTTCACTTATGATACTCACAACAATGATGAA
CCTGACAAAAGGCTGATGGAATTAAAATTTCCATCCCAAGATGAAATTCCGGATGACAGA
TTCTATACTCCAGCAGAGGAACCCACTGCTGAATTTCCGGTGGAAGAACTTCCAAATACT
CCGCGAAGTATTAACGTTACAACTTCAAATAACAAGAGCACAGACGATAAGTTGAGTAGC
GGTAATATTGATCAAAAACCTACCGAACTGTTAGATGATTTAGAATTCAGTTCATTTAAT
ATAGCATTTGGAAATACCTCCATGAGTACTGACAATATGAAAATATCATCCGACTTAAGT
TCGAATAAAACCGTGTTAGGAAATGCTCAGAAAGTTCAAGAGTCTCCTAGTGGACCATTA
ATCTATGTTTTGCCTCAGAGTAGCACAAAGCATGAGAAAGAGGGGTTCCTTCGAAAGAAA
CAAAAAGACGAGCCCATTTGGGTTTCCCCTAGCAAAATTGACTTTGCTGATCTAAGTAGG
AGAACTAAAGCATTGACGCCAGAGCGTAATACTGTTCCTTTGAAAAACAACGACAGTAGA
AAATACAAATATACTGGAGAGGGATCTATCGGTAATATGACAAATATGCTGTTAACTAAA
GATGCTTCGTACGCGTACTTAAAAGATTTTGTTGCGTTGAGTGACGATGAAGATGAAGAT
GGGAAACAGAACTGCGCTGTTGGTGGCCCAGAGAAACTGAAATTTTATTAG

Protein sequence predicted based on nucleotide sequence

MEKDLSSLYSEKKDKENDETLFNIKLSKSVVETTPLNGHSLFDDDKSLSDWTDNVFTQSV
FYHGSDDLIWGKFFVCVYKSPNSNKLNAIIFDKLGTSCFESVDISSNSQYYPAIENLSPS
DQESNVKKCIAVILLQRYPLLSPSDLSQILSNKSENCDYDPPYAGDLASSCQLITAVPPE
DLGKRFFTSGLLQNRFVSSTLLDVIYENNESTIELNNRLVFHLGEQLEQLFNPVTEYSPE
QTEYGYKAPEDELPTESDDDLVKAICNELLQLQTNFTFNLVEFLQKFLIALRVRVLNEEI
NGLSTTKLNRLFPPTIDEVTRINCIFLDSLKTAIPYGSLEVLKACSITIPYFYKAYTRHE
AATKNFSKDIKLFIRHFSNVIPEREVYTEMKIESIIKGPQEKLLKLKLIIERLWKSKKWR
PKNQEMAKKCYNNIIDVIDSFGKLDSPLHSYSTRVFTPSGKILTELAKCWPVELQYKWLK
RRVVGVYDVVDLNDENKRNLLVIFSDYVVFINILEAESYYTSDGSNRPLISDILMNSLIN
EVPLPSKIPKLKVERHCYIDEVLVSILDKSTLRFDRLKGKDSFSMVCKLSSAFISSSSVA
DLITKARILEKDTAFHLFKASRSHFTLYSTAHELCAYDSEKIKSKFALFLNIPPSKEILE
VNNLHLAFFARFCSNDGRDNIVILDVLTKHDDKHIEVTSDNIVFTIINQLAIEIPICFSS
LNSSMAKDLLCVNENLIKNLEHQLEEVKHPSTDEHRAVNSKLSGASDFDATHEKKRSYGT
ITTFRSYTSDLKDSPSGDNSNVTKETKEILPVKPTKKSSKKPREIQKKTKTNASKAEHIE
KKKPNKGKGFFGVLKNVFGSKSKSKPSPVQRVPKKISQRHPKSPVKKPMTSEKKSSPKRA
VVSSPKIKKKSTSFSTKESQTAKSSLRAVEFKSDDLIGKPPDVGNGAHPQENTRISSVVR
DTKYVSYNPSQPVTENTSNEKNVEPKADQSTKQDNISNFADVEVSASSYPEKLDAETDDQ
IIGKATNSSSVHGNKELPDLAEVTTANRVSTTSAGDQRIDTQSEFLRAADVENLSDDDEH
RQNESRVFNDDLFGDFIPKHYRNKQENINSSSNLFPEGKVPQEKGVSNENTNISLKTNED
ASTLTQKLSPQASKVLTENSNELKDTNNEGKDAKDIKLGDDYSDKETAKEITKPKNFVEG
ITERKEIFPTIPRLAPPASKINFQRSPSYIELFQGMRVVLDKHDAHYNWKRLASQVSLSE
GLKVNTEEDAAIINKSQDDAKAERMTQISEVIEYEMQQPIPTYLPKAHLDDSGIEKSDDK
FFEIEEELKEELKGSKTGNEDVGNNNPSNSIPKIEKPPAFKVIRTSPVRIIGRTFEDTRK
YENGSPSDISFTYDTHNNDEPDKRLMELKFPSQDEIPDDRFYTPAEEPTAEFPVEELPNT
PRSINVTTSNNKSTDDKLSSGNIDQKPTELLDDLEFSSFNIAFGNTSMSTDNMKISSDLS
SNKTVLGNAQKVQESPSGPLIYVLPQSSTKHEKEGFLRKKQKDEPIWVSPSKIDFADLSR
RTKALTPERNTVPLKNNDSRKYKYTGEGSIGNMTNMLLTKDASYAYLKDFVALSDDEDED
GKQNCAVGGPEKLKFY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4911
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable