Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH203014.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013260
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3390nts
Open reading frame: 1 to 3390
ATGGCCTATATGTTAGCTATTGCAAATTTTCACTTTTTCAAGTTCTATACTAGAATGAGA
AAAAAACACGAAAACAATTCTTGCAATGAAAAGGACAAAGATGAGAACCTATTCAAAATT
ATTCTCGCCATATTTTTGCAAGAAAAAAAAAAATACGATTGTATTTCATCTGGTTCAATT
ATGACTGCTTCAGAAGAGTACTTGGAGAATTTAAAACCGTTCCAGGTCGGTCTCCCTCCA
CATGATCCTGAATCGAATAAAAAACGTTACCTGCTGAAGGATGCTAATGGTAAAAAGTTT
GATCTGGAAGGCACTACAAAAAGATTTGAACATCTGCTATCCTTGAGTGGTTTATTCAAG
CATTTTATTGAAAGTAAAGCCGCTAAAGATCCCAAATTCAGGCAGGTCTTAGATGTATTA
GAAGAAAATAAAGCAAATGGCAAAGGCAAAGGCAAACACCAAGATGTTCGTAGACGAAAG
ACTGAACACGAAGAAGATGCGGAATTATTGAAAGAAGAAGACTCGGATGATGATGAGAGC
ATTGAATTCCAATTCAGAGAATCACCTGCTTACGTTAATGGTCAATTGAGACCTTATCAA
ATCCAAGGTGTCAACTGGTTGGTGTCTCTGCATAAGAACAAAATAGCGGGTATCCTTGCT
GATGAAATGGGTTTAGGTAAGACCCTGCAAACTATTTCATTTCTGGGATATCTTAGGTAC
ATTGAAAAAATTCCAGGTCCATTTTTAGTCATTGCGCCTAAGTCTACCTTAAATAATTGG
CTGAGAGAAATAAATAGATGGACGCCAGACGTAAACGCCTTTATTTTGCAAGGAGATAAA
GAAGAAAGAGCTGAACTGATCCAAAAAAAACTTTTAGGTTGTGACTTTGATGTTGTGATT
GCTTCTTATGAAATTATTATAAGAGAAAAGTCGCCCCTTAAGAAGATTAACTGGGAGTAT
ATTATCATTGATGAGGCTCACAGAATCAAGAATGAAGAGTCAATGCTTTCGCAAGTCTTA
AGGGAATTTACCTCACGTAATAGGCTGTTGATTACTGGTACTCCTTTGCAAAACAATTTG
CATGAACTATGGGCGTTATTAAATTTTTTGTTACCAGATATCTTTTCCGATGCACAAGAT
TTCGACGATTGGTTTTCTTCTGAAAGCACAGAGGAAGATCAGGATAAAATTGTCAAGCAA
CTCCACACTGTTTTACAGCCTTTTCTACTACGTCGTATCAAAAGCGATGTGGAAACATCC
TTACTGCCTAAAAAGGAACTGAATTTATATGTCGGTATGTCTAGCATGCAGAAGAAATGG
TACAAAAAGATTTTGGAAAAGGATCTGGACGCTGTGAACGGGTCTAATGGCAGCAAGGAA
TCCAAAACAAGACTTTTAAATATCATGATGCAACTTAGAAAATGTTGTAATCATCCATAT
TTATTCGATGGTGCTGAGCCAGGTCCACCGTACACTACGGATGAGCACTTGGTTTATAAC
GCTGCCAAACTTCAAGTTTTGGATAAGTTATTGAAGAAATTGAAGGAAGAAGGTTCTAGA
GTTCTGATTTTCAGTCAAATGTCAAGATTATTGGATATTCTCGAGGATTATTGCTACTTC
AGAAACTATGAATATTGCAGGATCGACGGTTCCACCGCTCACGAAGATAGAATTCAAGCC
ATTGATGATTATAATGCTCCTGATTCTAAGAAGTTTGTTTTCTTATTAACGACACGTGCA
GGTGGGTTAGGTATCAATTTAACGTCAGCAGATGTCGTTGTCTTGTATGATTCAGATTGG
AACCCACAAGCAGATTTACAAGCAATGGATAGAGCGCATCGTATTGGTCAGAAGAAGCAG
GTCAAGGTATTCCGTTTGGTCACAGATAACTCCGTGGAAGAGAAAATTTTAGAAAGGGCT
ACCCAGAAGTTAAGATTAGATCAATTAGTTATCCAGCAAAACAGGACTTCTTTGAAAAAG
AAGGAAAATAAAGCAGATTCCAAAGATGCTTTATTATCCATGATTCAACATGGTGCCGCC
GATGTATTCAAAAGTGGTACATCTACCGGTTCAGCTGGTACGCCGGAACCTGGTTCAGGA
GAAAAAGGAGATGATATTGATCTGGACGAACTTTTACTGAAATCAGAAAACAAAACTAAG
TCATTAAATGCAAAATATGAAACTTTAGGTCTTGATGATTTGCAAAAATTCAACCAAGAT
TCGGCCTATGAATGGAATGGTCAAGATTTCAAGAAGAAAATACAAAGGGATATTATAAGT
CCGCTACTGCTCAACCCCACCAAACGTGAACGTAAGGAAAATTACTCTATTGATAATTAT
TATAAAGATGTTTTGAATACAGGAAGGTCCTCGACCCCATCACACCCCAGAATGCCTAAG
CCTCATGTCTTCCATTCCCATCAATTACAACCTCCCCAATTGAAAGTCCTGTACGAAAAG
GAAAGAATGTGGACGGCGAAGAAGACTGGTTATGTACCGACCATGGATGACGTAAAAGCA
GCATATGGCGATATTTCAGATGAAGAAGAAAAGAAACAAAAATTGGAATTGTTAAAATTG
TCGGTTAACAACTCGCAACCATTAACTGAAGAGGAAGAAAAGATGAAGGCTGATTGGGAG
TCTGAAGGATTTACAAATTGGAATAAATTAGAATTTAGAAAATTCATCACGGTATCTGGT
AAATACGGTAGGAACTCCATTCAAGCCATTGCGAGAGAATTGGCCCCTGGCAAAACACTA
GAGGAAGTTCGCGCATATGCTAAGGCCTTCTGGTCTAACATTGAAAGAATTGAAGATTAT
GAGAAATATCTGAAAATAATTGAGAATGAAGAAGAGAAGATCAAGCGTGTCAAAATGCAA
CAAGAGGCTTTACGTCGGAAGTTGTCTGAGTATAAGAATCCATTCTTCGATTTGAAATTA
AAACATCCTCCTTCTTCTAACAACAAAAGGACGTACTCTGAAGAAGAAGATCGTTTTATC
CTTCTCATGTTATTCAAGTATGGCTTGGATAGAGATGACGTATACGAATTAGTGCGTGAT
GAAATTAGAGATTGTCCCCTATTCGAATTAGACTTTTACTTCAGAAGCAGGACACCTGTA
GAGCTAGCCAGAAGAGGAAATACATTACTGCAATGCTTGGAAAAGGAATTTAACGCAGGG
ATTGTATTGGATGACGCCACTAAAGATAGAATGAAGAAAGAAGATGAAAATGGTAAAAGG
ATAAGGGAAGAATTTGCAGATCAGACCGCTAATGAGAAGGAGAACGTTGATGGAGTAGAG
AGCAAAAAAGCGAAGATTGAGGATACGTCGAATGTTGGAACTGAACAGTTGGTAGCAGAG
AAAATTCCGGAAAACGAAACCACTCATTAG
Protein sequence predicted based on nucleotide sequence
MAYMLAIANFHFFKFYTRMRKKHENNSCNEKDKDENLFKIILAIFLQEKKKYDCISSGSI MTASEEYLENLKPFQVGLPPHDPESNKKRYLLKDANGKKFDLEGTTKRFEHLLSLSGLFK HFIESKAAKDPKFRQVLDVLEENKANGKGKGKHQDVRRRKTEHEEDAELLKEEDSDDDES IEFQFRESPAYVNGQLRPYQIQGVNWLVSLHKNKIAGILADEMGLGKTLQTISFLGYLRY IEKIPGPFLVIAPKSTLNNWLREINRWTPDVNAFILQGDKEERAELIQKKLLGCDFDVVI ASYEIIIREKSPLKKINWEYIIIDEAHRIKNEESMLSQVLREFTSRNRLLITGTPLQNNL HELWALLNFLLPDIFSDAQDFDDWFSSESTEEDQDKIVKQLHTVLQPFLLRRIKSDVETS LLPKKELNLYVGMSSMQKKWYKKILEKDLDAVNGSNGSKESKTRLLNIMMQLRKCCNHPY LFDGAEPGPPYTTDEHLVYNAAKLQVLDKLLKKLKEEGSRVLIFSQMSRLLDILEDYCYF RNYEYCRIDGSTAHEDRIQAIDDYNAPDSKKFVFLLTTRAGGLGINLTSADVVVLYDSDW NPQADLQAMDRAHRIGQKKQVKVFRLVTDNSVEEKILERATQKLRLDQLVIQQNRTSLKK KENKADSKDALLSMIQHGAADVFKSGTSTGSAGTPEPGSGEKGDDIDLDELLLKSENKTK SLNAKYETLGLDDLQKFNQDSAYEWNGQDFKKKIQRDIISPLLLNPTKRERKENYSIDNY YKDVLNTGRSSTPSHPRMPKPHVFHSHQLQPPQLKVLYEKERMWTAKKTGYVPTMDDVKA AYGDISDEEEKKQKLELLKLSVNNSQPLTEEEEKMKADWESEGFTNWNKLEFRKFITVSG KYGRNSIQAIARELAPGKTLEEVRAYAKAFWSNIERIEDYEKYLKIIENEEEKIKRVKMQ QEALRRKLSEYKNPFFDLKLKHPPSSNNKRTYSEEEDRFILLMLFKYGLDRDDVYELVRD EIRDCPLFELDFYFRSRTPVELARRGNTLLQCLEKEFNAGIVLDDATKDRMKKEDENGKR IREEFADQTANEKENVDGVESKKAKIEDTSNVGTEQLVAEKIPENETTH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3390
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
