leuA (Y. pestis KIM10+ )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  leuA
Gene Name:  2-isopropylmalate synthase
Original Clone ID:
FLH122983.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
YpCD00013633
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1563nts
Open reading frame: 1 to 1563

ATGAGCCAACAAGTCATTATTTTTGATACAACATTGCGCGACGGTGAACAAGCATTGCAA
GCCAGTCTGAGTGTGAAAGAGAAGCTGCAAATCGCGCTGGCATTGGAAAGAATGGGGGTC
GATATCATGGAGGTGGGTTTCCCTGTCTCCTCCCCTGGTGATTTTGAATCGGTTCGTACT
ATCGCTCAGCAAGTGAAAAATAGCCGGGTTTGCGCCTTGGCACGTTGTGTGGATAAAGAT
ATTGATGTTGCGGCAGAGGCATTGCGAATTGCAGAAGCTTTCCGTATTCATGTGTTTTTG
GCCACCTCAACCTTGCATATCGAATCCAAGTTAAAGCGTTCGTTTGACGATGTGCTGGCG
ATGGCGGTCCACTCCGTGAAACGTGCTCGTAACTATACCGATGACGTCGAGTTTTCTTGT
GAAGATGCTGGCCGTACTCCAATTGATAACTTATGCCGTGTCGTCGAGGCCGCGATTACC
GCCGGTGCCACCACAATCAATATCCCGGATACCGTCGGTTACACCCCCCCTTATCAATTT
GGTGGCATCATCACTGACTTGTATGAACGTGTTCCCAATATTGATAAAGCCATTATCTCT
GTTCATTGCCATGATGACTTGGGCATGTCCGTTGCCAACTCCATTACTGCAGTACAAGCC
GGGGCGCGTCAGGTCGAAGGGACCATCAACGGCTTAGGTGAGCGTGCAGGTAACTGTTCA
TTGGAAGAGGTGATCATGGCCATTAAAGTGCGCCATGAAATGTTGGGCGTGCACACCAAT
ATCAATCATCAGGAAATTTACCGTACCAGCCAGTTGGTCAGTAAAATTTGCAATATGCCT
ATTCCCGGCAATAAAGCCATTGTCGGCAGTAATGCCTTCACTCACTCCTCCGGTATTCAT
CAGGATGGTGTCCTGAAAAATCGCGAAAACTACGAAATCATGACCCCGGAATCCATCGGT
CTGAAAGAAGTCCAATTGAACCTGACATCCCGTTCAGGTCGTGCGGCAGTGAAGCACCGG
ATGGAAGAGATGGGTTATCAGGATAAAGATTACAATTTAGATTCTTTGTACGATGCCTTC
TTGAAATTAGCCGATAAAAAAGGCCAGGTCTTTGACTACGATTTGGAAGCATTGGCCTTT
ATTAATAAGCAGCAGGAAGAGCCTGAATATTATCGTTTGGACTATTTCAGCGTCCAGTCG
GGTTCCAGTGTCATGGCAACTGCCTCAGTAAAATTGGTCTGTGGTGAAGAAATAAAATCA
GAAGCGGCCACCGGTAACGGCCCGGTTGATGCGGTGTATCAGGCCATCAATCGCATTACC
GACTACCCAATTGAACTGGTGAAGTATCAACTGTCGGCCAAAGGTCAGGGTAAAGATGCA
TTGGGTCAGGTTGATATCGTGGTTGACCACAAGGGGCGTCGCTTCCATGGCGTAGGTTTG
GCAACTGACATTGTTGAGTCATCCGCTAAGGCGTTGGTTCACGTATTAAATAACATCTGG
CGCGCCCATCAGGTCGAAAAAGAGAAGCAGCGTCTGCAACAAAATAATCAGGAAATGGTG
TAG

Protein sequence predicted based on nucleotide sequence

MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRT
IAQQVKNSRVCALARCVDKDIDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLA
MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAITAGATTINIPDTVGYTPPYQF
GGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS
LEEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFTHSSGIH
QDGVLKNRENYEIMTPESIGLKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAF
LKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQSGSSVMATASVKLVCGEEIKS
EAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGL
ATDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1563
Mutations:
None
Discrepancy:
g880a,A294T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable