pntA (Y. pestis KIM10+ )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  pntA
Gene Name:  pyridine nucleotide transhydrogenase, alpha subunit
Original Clone ID:
FLH136568.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YpCD00015901
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1527nts
Open reading frame: 1 to 1527

ATGCGTATTGGTGTACCAAGAGAGCGGTTGGCCAATGAAGCCAGGGTTGCAGCAACGCCG
AAAACGGTGGAACAATTGCTGAAATTAGGCTTCACCGTGGCAATTGAAAGTGGAGCGGGG
CATTTAGCCAGCTTTGACGATGCGGCGTACCAAGAAGCGGGTGCCATCATTACGGATACC
ACTGATGTGTGGCAATCCGATCTTATCCTGAAGGTGAATGCTCCCTTTGAGGATGAAATT
GCGCTGATGCGTGAGGGGAGCACGTTGGTCAGTTTTATTTGGCCCGCTCAGAACCCTGAG
TTGCTGCAAAAACTGGCAGAGCGTCAAGTTACTGCATTAGCGATGGATTCAGTGCCGCGT
ATCTCCCGTGCGCAATCCATGGATGCCCTCAGCTCCATGGCAAATATTGCCGGTTACCGT
GCCATTGTTGAAGCGGCTCATGAATTTGGCCGTTTCTTCACCGGGCAGATCACCGCGGCG
GGTAAAGTTCCACCTGCGAAGGTGATGGTTATTGGTGCCGGAGTCGCTGGTCTGGCGGCT
ATCGGTGCGGCGGGGAGCCTCGGGGCTATCGTGCGTGCTTTTGACACCCGTCCTGAAGTA
AAAGAACAAGTCCAGAGTATGGGCGCTGAATTCCTTGAGTTGGATTTTGAAGAGGAAGCG
GGCAGTGGCGATGGTTACGCCAAAGTGATGTCCGAGGCCTTTATCAAAGCTGAGATGGAA
CTGTTTGCTGCGCAAGCCGCAGAAGTAGACATTATCGTCACTACCGCGCTGATCCCTGGT
AGACCAGCACCGCGCCTGATCACCAAAGAGATGGTGGCGTCGATGAAGTCGGGCAGCGTG
ATTGTCGATCTGGCGGCTCAGACAGGCGGTAACTGTGAGTTGACGGTAGCTGATGAAGTC
ACCATCACGGAAAATGGCGTAAAAATCATTGGCTATACAGATCTGCCAAGCCGCTTACCG
ACGCAGTCTTCACAGCTCTATGGTACTAACCTCGTTAACTTATTGAAGTTACTTTGCAAA
GAGAAAAACGGCGAGATCGATATTGATTTCGACGATACCGTGATTCGCGGTGTTACTGTG
GTTAAAACTGGCGAAGTGACTTGGCCGGCACCACCTATTCAGGTTTCAGCTCAGCCGCAG
GCTGCCAAAGCGGCCCCAATAGCGAAGGAAGAAACGAAGCCCTCATCACCGTGGACCAAA
TACATCATTATGGCAGTTGCTATTGTGTTGTTTGGTTGGTTGGCGAATGTGGCACCACCA
GAATTCTTATCTCACTTTACGGTCTTTGCACTGGCGTGTGTAGTCGGTTACTACGTGGTA
TGGAATGTCAGTCATGCGCTGCATACACCGCTGATGTCAGTCACTAACGCGATTTCGGGC
ATTATTGTGGTTGGCGCGTTGTTGCAGATTGGTCATGGTGGCTGGGTAAGTTTCTTCTCC
TTTATTGCCGTACTGATTGCTAGCATCAACATTTTTGGTGGCTTCACTGTCACCCAGCGC
ATGCTGAAAATGTTCCGTAAAAACTAG

Protein sequence predicted based on nucleotide sequence

MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGHLASFDDAAYQEAGAIITDT
TDVWQSDLILKVNAPFEDEIALMREGSTLVSFIWPAQNPELLQKLAERQVTALAMDSVPR
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEME
LFAAQAAEVDIIVTTALIPGRPAPRLITKEMVASMKSGSVIVDLAAQTGGNCELTVADEV
TITENGVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLCKEKNGEIDIDFDDTVIRGVTV
VKTGEVTWPAPPIQVSAQPQAAKAAPIAKEETKPSSPWTKYIIMAVAIVLFGWLANVAPP
EFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFFS
FIAVLIASINIFGGFTVTQRMLKMFRKN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1527
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable