STK3 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  STK3
Gene Name:  serine/threonine kinase 3
Original Clone ID:
FLH000105.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00001653
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1476nts
Open reading frame: 1 to 1476

ATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGACAGTTTGACT
AAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGT
GTATTTAAAGCAATACACAAGGAATCCGGTCAAGTTGTCGCAATTAAACAAGTACCTGTT
GAATCAGATCTTCAGGAAATAATCAAAGAAATTTCCATAATGCAGCAATGTGACAGCCCA
TATGTTGTAAAGTACTATGGCAGTTATTTTAAGAATACAGACCTCTGGATTGTTATGGAG
TACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGATTACGAAACAAGACATTAATAGAA
GATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGACTAGAATATTTGCACTTTATG
AGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACATGCA
AAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACT
GTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGT
GTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCT
TATGCTGATATACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACA
TTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATTTTGTTAAAAAGTGTTTGGTG
AAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAAT
GCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAA
AGACATGAGGAACAGCAACGAGAATTGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAG
CTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGGCCACA
AGCACGATGAGTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCC
GACTTGGGGACCATGGTGATAAACAGTGAGGATGAGGAAGAAGAAGATGGAACTATGAAA
AGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATAAGCAA
GACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATG
TCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAGATGGAGACTTTGACTTTTTG
AAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAA
CGGGAGATAGAAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCG
ATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTTG

Protein sequence predicted based on nucleotide sequence

MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPV
ESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIE
DEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNT
VIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPT
FRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAK
RHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLES
DLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPM
SKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDA
MDAKKRRQQNFL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1551
Start on reference sequence 76
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable