Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  putative ATP-dependent protease
Original Clone ID:
FLH140803.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YpCD00016729
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2604nts
Open reading frame: 1 to 2604

ATGGGAGCGATAACGGTGTATCAACGCGAACGTCTTTTTAATCGATTGGGCACTTTTGCC
TATAAAACTTTTGTTGAAGCCACCCGTCTTTGCCGCAGTTATCGGCATGAGTATGTGGAG
CTTGAACATTGGTTAAAAATTCTCATGGATCAACAGCGTGGGGATATTCCTGCTCTACTG
ACGCATTACGGACTGAGCCAGACGGTTATCTCCACGCAATTGGATCGCATTATTCAGCAT
ATGCCCGTCACCAAAGCATCGGTGCAGGATTTATCATCACGCTTGGAGTCCGTGGTCGAA
GGCGGATTGGTGTTGAGCCAATTGATGGCGTCACCTTCTCCTATCCGTACCTCGCATATT
TTACTGGCACTATTACAGGATACCCAGCATCAGCGCTGGTTATACCGCCTGTGCGATGAA
TTCAAAAAGCTGCCGGTCCCACAGGTTGCAGAGGAATATGAAGGCCTATTAGTTAACTCC
ATTGAACAGACCAGCGAGGTTCAAAGAGGCGACGCATTAGTGACGGAAACGGACAGCAGC
TCATCAAAAGAAGCGCATGCGGCATTGAACAAATGGTGTAACGACCTTACCCAGCAAGCC
CGTGACGGTGAGATTGACCCCGTTATTGGTCGTGAAGCAGAACTGCGTCAAGTGATTGAT
ATTTTACTCCGTCGCCGTCAGAACAACCCTATTTTGGTTGGGGAAGCGGGGGTCGGTAAA
ACGGCGGTGGTTGAAGCGCTAGCGCGAAAACTGGCCAGTGGCGATGTCCCTCCGCTGTTG
CAAGGGGCGCGTCTGCTTTCTCTGGATTTGGGGAGGATGCAAGCCGGTGCCAGTATGCGC
GGTGAATTTGAATCACGTCTTAAATCCCTGATCGATGGCATCAGCCAATCGGATACCCCG
GTTATTCTGTTTTGTGATGAAGCCCATACGTTGGTGGGGGCAGGCGGGCAGGCGGGCACG
GGGGATGCGGTCAACCTGCTTAAACCGATGTTGGCGAGGGGCGCATTGCGCATGATCGCC
GCCACCACCTGGTCAGAGTATAAACAGTTTATTGAACCTGACGCCGCGCTGACCCGGCGT
TTCCAGCGGGTATTGATTGGCGAGCCAAACGAATCTACCGCCGTCGATATGCTACGGGCG
ATTGCGCCACATTTTGCTCAACATCATAACGTCACTATCCGCGACGGTGCCATTCACGCG
GCGGTGCGCCTGTCATTACGCAACCTGCCTTCCCGGCAGTTGCCCGATAAAGCCATCAGT
CTGCTCGACACCGCTTGCGCGCGGGTCGCGCTCAGCCAATATGCCCAACCACAGGCGATT
GAACAGTTATCGGCCCAACTGGATGTGCTCAAAACCGAGCATCAATATCTGGTACGGGAG
AAGCAACTGGGCGAAGCGGTTGATCAACGGCTAGATGACGTTCAGACACAGATCCAGGCG
TTTGAACATGAACTAACCGCCTTGCAAAGCCGCCATCTGCATGAGCAACAGCTAGTGAGG
GAGGTCCTGCCGGAGGGAGAAGGCAGTGGCATGATGACGGAAAATGCCCGCTGGGGTGAA
CTGATGGCGTTACAGGAAGCGTCGCCACTGGTCTATCCATGGGTTGATGAGCAGAGCATC
GCCGCGGTGCTTTCCGATTGGACCGGGATCCCGTCCGGCAAAATGTTGCAGGACGATATT
GAATGCGTCCTGAATCTTGAACAGCGTCTCGGTGATCATATTTTCGGGCAACGCAACGCT
ATCAAAGAGATTTCGCAGGCGATACGTATTGCTCGGGCGGGCATCCAGTCTCAGGAGCGG
CCACTCGGGATCTTCCTGCTGGCGGGGTCAACCGGTACGGGCAAAACAGAAACGGCCAAT
GTACTGGTCGAAACCTTGTATGGCGGTGCTCATAACCTTATTACCTTCAATATGAGTGAG
TTCCAAGAGGCGCATACCTTATCCACGCTGAAAGGCGCACCTCCGGGCTATGTCGGCTAT
GGCAAGGGGGGGAAATTAACCGAAGCGGTAAGACGCAAACCTTATTCGGTCATCCTGCTT
GATGAGTTTGATAAAGCTCACGCCGATATTCAGGACGCCTTTTATCAGGTCTTCGACAAA
GGGTGGATGGAAGATGCCGAAGGGCGTCGGGTCAGTTTCCGCCAGTGTTTCATCTTACTG
ACCTGTAATCAGGGCGCGGAAGAGATCGAACAGGCTTACCTCACCGCCAACGATATCAAA
CCCGGTGCATTAAAACCGTTGGTATACGACGCCCTGTTGCGCCGCTTTGCGCCAGCGCTG
TTAGCCAGGGTCAATATCATTCCTTATATCCCATTAGATCAGGACGCTCTGGCACAGATC
GCCTCTCATCATCTTGCGCGCTTACAAACGCGCCTGTGGGATGAAATTGGTGCCACTTTG
GTGACCGAGGGGGATATCCCCGGCTGGATCGCCTCACGGGTTTGCAGCCATCCGAACCAT
GGTCGTGCGGTGGAAGAACTCTTGCGTCAAACCTTACTTCCCGCTGTTGGCAATGAAGTG
TTGAAGCGTCGCCATGAGGCTGAACCGCTGAGAGAGATACGCCTGATTGTTGAGGAGACA
GAACTGAGTATTGAGTTCGCGTAG

Protein sequence predicted based on nucleotide sequence

MGAITVYQRERLFNRLGTFAYKTFVEATRLCRSYRHEYVELEHWLKILMDQQRGDIPALL
THYGLSQTVISTQLDRIIQHMPVTKASVQDLSSRLESVVEGGLVLSQLMASPSPIRTSHI
LLALLQDTQHQRWLYRLCDEFKKLPVPQVAEEYEGLLVNSIEQTSEVQRGDALVTETDSS
SSKEAHAALNKWCNDLTQQARDGEIDPVIGREAELRQVIDILLRRRQNNPILVGEAGVGK
TAVVEALARKLASGDVPPLLQGARLLSLDLGRMQAGASMRGEFESRLKSLIDGISQSDTP
VILFCDEAHTLVGAGGQAGTGDAVNLLKPMLARGALRMIAATTWSEYKQFIEPDAALTRR
FQRVLIGEPNESTAVDMLRAIAPHFAQHHNVTIRDGAIHAAVRLSLRNLPSRQLPDKAIS
LLDTACARVALSQYAQPQAIEQLSAQLDVLKTEHQYLVREKQLGEAVDQRLDDVQTQIQA
FEHELTALQSRHLHEQQLVREVLPEGEGSGMMTENARWGELMALQEASPLVYPWVDEQSI
AAVLSDWTGIPSGKMLQDDIECVLNLEQRLGDHIFGQRNAIKEISQAIRIARAGIQSQER
PLGIFLLAGSTGTGKTETANVLVETLYGGAHNLITFNMSEFQEAHTLSTLKGAPPGYVGY
GKGGKLTEAVRRKPYSVILLDEFDKAHADIQDAFYQVFDKGWMEDAEGRRVSFRQCFILL
TCNQGAEEIEQAYLTANDIKPGALKPLVYDALLRRFAPALLARVNIIPYIPLDQDALAQI
ASHHLARLQTRLWDEIGATLVTEGDIPGWIASRVCSHPNHGRAVEELLRQTLLPAVGNEV
LKRRHEAEPLREIRLIVEETELSIEFA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2604
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable