Gene:
Gene Symbol:
hrpA
Gene Name:
helicase, ATP-dependent
Original Clone ID:
FLH141068.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YpCD00016781
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3888nts
Open reading frame: 1 to 3888
ATGAAATCTTCGCTCACAGCATTATCCACTCAACTTGGTGAGTTGATGCTCCGTGACCAA
CAGCGCATTCAGCGTCGGCTACATAGCGCACGAAAAATCAATAACCCTGACGCCATACAG
GCGATTACTCACGAAATTGAAGCCGAAATCGCGATAGCAATGCAGCGAGTAACCCGCCGT
CGGGCGGCTTGTCCGGCGATCAGTTATCCAGATAATCTGCCCGTCAGCCAGAAGAAACAG
GATATTTATAACGCTATCCGTGACCATCAGGTGATCATTGTCGCCGGTGAGACTGGGTCG
GGTAAAACGACACAGTTGCCGAAAATCTGCCTGGAACTGGGGCGGGGGGTGAAAGGCGTT
ATCGGCCACACTCAGCCACGCCGTTTGGCCGCCCGGACGGTGGCTAACCGCATTGCCGAT
GAGCTGGATACTTCGCTTGGCGGCTGTGTTGGTTACAAAGTTCGTTTTAACGATCAGGTC
GGTGAAAATACACTGGTCAAATTAATGACGGATGGCATTCTGCTGGCTGAAATTCAGCAA
GATCGCCTGCTGATGCAATATGACACGTTGATCATTGATGAGGCCCACGAACGTAGCCTT
AACATTGATTTTATCTTGGGCTATTTACGTGAGTTATTACCTAAACGCCCTGATCTGAAA
GTGATCATTACGTCAGCGACTATTGACCCGCAACGCTTCTCACAACATTTCAATAACGCC
CCAATTATTGAGGTCTCTGGCCGTACCTACCCGGTGGAGGTGCGCTACCGGCCGATTGTG
GATGATGCCGATGATGTTGATCGGGACCAATTGCAGGCCATTTTTGATGCAGTCGATGAG
TTGGGCCGTGAAAGCGCCGGGGATATCTTAATCTTTATGAGTGGTGAGCGCGAAATCCGT
GACACAGCGGATGCGTTGATAAAGCAAAATTTGCCACACACCGAAGTGCTACCGCTGTAT
GCCCGCTTATCAAACAGTGAGCAGAACCGGGTGTTTCAGTCACATCATGGGCGGCGAATT
GTGTTGGCAACCAACGTGGCAGAAACGTCACTCACGGTGCCGGGTATCAAATATGTTATC
GATCCCGGCACAGCACGTATCAGCCGTTACAGTTTCCGTACCAAGGTCCAGCGGTTACCG
ATTGAGCCTATTTCTCAGGCATCGGCTAATCAGCGTAAAGGGCGGTGTGGCCGTGTCTCT
GACGGTATTTGTATTCGTCTTTATTCCGAGCAAGATTTTCTCTCCCGCCCTGAGTTCACG
GATCCAGAAATCCTACGGACCAATCTGGCTTCGGTGATCTTGCAAATGACCTCTCTGGGG
TTAGGGGATATCGCTGCATTTCCCTTTGTTGAAGCACCGGATAAACGCAATATCCAAGAC
GGTGTAAGGTTACTGGAGGAACTGGGGGCCATTCAGACCGCCAGTAACGGGCATCAGCAA
TTGACCCCATTGGGCCGCCAGTTAGCCCAACTTCCTGTCGATCCACGCTTGGCCAGAATG
GTATTGCAAGCGCAGAAAAGTGGCTGCGTACGTGAATTAATGATTATCACCTCGGCGTTA
TCTATTCAGGATCCGCGTGAGCGTCCGGTAGATAAACAGCAGGCCTCTGATGAAAAACAC
CGCCGTTTTGCGGATAAAGATTCCGATTTTCTCGCCTTTGTTAATCTGTGGGATTATCTC
AAAGAACAACAAAAAGCGTTGTCATCAGCACAATTTCGTAAGCTTTGCCGCAGCGATTAC
CTGAATTACCTACGGGTGCGTGAATGGCAGGATATTTACACTCAGTTGCGGCAGGTCGTT
AAAGAGTTAGGGATACCCGTCAACAGTGTTGCCGCAGACTACCGCAGTGTACATACCGCG
ATACTGACCGGTTTACTTTCTCACATTGGGCAGAAAGATGTTGAGAAGCAAGAGTTTACC
GGTGCTCGTAACGCCCGCTTTGCTATTTTCCCCGGCTCCGGCTTATTCAAAAAGCCCCCC
AAGTGGGTCATGGTGGCTGAATTGGTCGAGACCAGCCGTTTATGGGGGCGCATTGCTGCA
CGTATTGAACCCGAATGGATTGAGCCTTTAGCCCAGCACCTGGTGAAACACCATTACAGC
GATCCACATTGGGAGAAAGCTCAGGGCGCGGTAATGGCCAGCGAGAAAGTGACCTTATTC
GGCTTACCGATTGTTACGGAGCGTAAGATCAATTACGGCCCGATCGATCCACTGCTCTGC
CGTGAGTTGTTTATTCGCCACGGGTTGGTTGAAGGTGACTGGCAGACGCGGCACGCGTTC
TTCCGAATGAATCTTAAGCTATTGGCTGAAGTGGAAGAGTTGGAACACAAATCCCGTCGG
CGGGATATTCTGGTCGATGACGAGACACTCTTTAACTTTTATGATCAAAGAATTGGTCGT
GATGTTATCTCTGCCCTCCATTTTGATAGCTGGTGGAAGCAAGCGAGCCAGACGAAACCC
GAGCTGCTGAACTTTGAAAAAACCATGCTTATCAAAGAGGGTGCGAACAAAGTAAACCCG
CTTGATTATCCGAATTTTTGGTATCAAGGGGAGCTAAAACTCCGTCTGTTTTATCAGTTT
GAACCCGGCACCGATGCCGATGGTGTCACAGTACACATTCCATTACCTATTCTGAACCAG
ATCCATGAGCAGGGGTTTGACTGGCAGATCCCAGGGATTCGCCGCGAACTGGTGATAGCA
CTGATTAAATCATTGCCCAAACCGGTACGGCGTAATTTTGTTCCGGCACCTAATTATGCC
GATGCTTTTCTGGCACGGGTAACACCACTCGAAACGGGTTTATTGGAGGCTTTGGAGCGT
GAATTACGCCGCATGACTGGCGTGACGGTGTCACGTGATAGTTGGCAGTTAGATCAGGTG
CCTGACCATCTGAAAATAACGTTTCGTGTGCTGGACGATAAAAACCGTATTTTGCGAGAA
GGCAAAGATCTTGCCGCCCTAAAGTTACAGTTGCAAGAGAAAGTACAGGAAACACTTTCT
GCCGTCGCGGATGATGGTATTGAACAAAATAACTTACATATCTGGAGTTTTGGCGATCTC
CCTATCTGCTATGAGCAACGGCGCGGTGGCTATGAAGTCAAAGCCTATCCAGCGTTAGTG
GATGAAAAAGACAGTGTTGCCATCCGTTTGTTCGATACAGAATCGCAACAACAACAGGCT
ATGTGGCAGGGTACCCGGCGTTTATTGCTACTCAATATTCCTTCGCCGATTAAGTACTTA
CATGAGAAATTGCCGAATAAATCCAAACTGGGTCTCTATTTCAATACGTATGGCAACGTA
ATGGATCTGATTGATGACTGCATCGCCTGTGGCGTCGATAAACTGGTGGCACAGCATGGT
GGCCCGGTCTGGCAGGAGGCTGATTTTGCTCGTTTACAAGAAAAAGTCCGTGCGGAACTG
AATGACACCGTGGTGGATATTGCCAAGCAGGTTGAGCAGATCCTCACGCTGGTGTTCAGT
ATTAATAAGCGGTTGAAAGGCCGGATCGATATTTCACTGGTATTGGCGCTTTCTGATATT
AAAGCTCAGCTTGCTGGCCTTATCTATCGTGGATTTGTCACGAGTAATGGCTGGAAGCGT
TTGCCTGATACTTTACGTTATTTAAAGGCTATTGAGCGGCGCTTAGAAAAACTGGCGGTT
GATCCTCATCGCGATAGGGCGCAAATGCTGCGAATTGAGCATGTTCAGCAAGTATGGCAA
CAGTGGTTGAATAAATTACCGCCTAAACGTCAGCAAGATGAAGAGGTTAAAGAAGTCCGT
TGGATGATTGAAGAACTGCGTGTCAGTTTATTTGCTCAACAACTGGGCACCCCTTATCCG
GTGTCTGACAAACGTATCTTACAAACGATCGAACAGCTTTCTGTCTAG
Protein sequence predicted based on nucleotide sequence
MKSSLTALSTQLGELMLRDQQRIQRRLHSARKINNPDAIQAITHEIEAEIAIAMQRVTRR RAACPAISYPDNLPVSQKKQDIYNAIRDHQVIIVAGETGSGKTTQLPKICLELGRGVKGV IGHTQPRRLAARTVANRIADELDTSLGGCVGYKVRFNDQVGENTLVKLMTDGILLAEIQQ DRLLMQYDTLIIDEAHERSLNIDFILGYLRELLPKRPDLKVIITSATIDPQRFSQHFNNA PIIEVSGRTYPVEVRYRPIVDDADDVDRDQLQAIFDAVDELGRESAGDILIFMSGEREIR DTADALIKQNLPHTEVLPLYARLSNSEQNRVFQSHHGRRIVLATNVAETSLTVPGIKYVI DPGTARISRYSFRTKVQRLPIEPISQASANQRKGRCGRVSDGICIRLYSEQDFLSRPEFT DPEILRTNLASVILQMTSLGLGDIAAFPFVEAPDKRNIQDGVRLLEELGAIQTASNGHQQ LTPLGRQLAQLPVDPRLARMVLQAQKSGCVRELMIITSALSIQDPRERPVDKQQASDEKH RRFADKDSDFLAFVNLWDYLKEQQKALSSAQFRKLCRSDYLNYLRVREWQDIYTQLRQVV KELGIPVNSVAADYRSVHTAILTGLLSHIGQKDVEKQEFTGARNARFAIFPGSGLFKKPP KWVMVAELVETSRLWGRIAARIEPEWIEPLAQHLVKHHYSDPHWEKAQGAVMASEKVTLF GLPIVTERKINYGPIDPLLCRELFIRHGLVEGDWQTRHAFFRMNLKLLAEVEELEHKSRR RDILVDDETLFNFYDQRIGRDVISALHFDSWWKQASQTKPELLNFEKTMLIKEGANKVNP LDYPNFWYQGELKLRLFYQFEPGTDADGVTVHIPLPILNQIHEQGFDWQIPGIRRELVIA LIKSLPKPVRRNFVPAPNYADAFLARVTPLETGLLEALERELRRMTGVTVSRDSWQLDQV PDHLKITFRVLDDKNRILREGKDLAALKLQLQEKVQETLSAVADDGIEQNNLHIWSFGDL PICYEQRRGGYEVKAYPALVDEKDSVAIRLFDTESQQQQAMWQGTRRLLLLNIPSPIKYL HEKLPNKSKLGLYFNTYGNVMDLIDDCIACGVDKLVAQHGGPVWQEADFARLQEKVRAEL NDTVVDIAKQVEQILTLVFSINKRLKGRIDISLVLALSDIKAQLAGLIYRGFVTSNGWKR LPDTLRYLKAIERRLEKLAVDPHRDRAQMLRIEHVQQVWQQWLNKLPPKRQQDEEVKEVR WMIEELRVSLFAQQLGTPYPVSDKRILQTIEQLSV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3888
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
