fusA (Y. pestis KIM10+ )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  fusA
Gene Name:  GTP-binding protein chain elongation factor EF-G
Original Clone ID:
FLH141917.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
YpCD00016814
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2109nts
Open reading frame: 1 to 2109

ATGGCTCGTAAAACACCCATTGAGCGCTATCGTAATATCGGTATCAGCGCTCACATCGAC
GCCGGTAAGACAACCACTACCGAACGTATCCTGTTTTACACCGGTGTAAATCACAAAATC
GGTGAAGTTCATGACGGCGCAGCCACCATGGACTGGATGGAACAGGAGCAGGAGCGTGGT
ATTACCATTACTTCTGCAGCTACTACCTGCTTCTGGTCTGGTATGGCTAAACAGTTCGAA
CCACATCACGTCAATATCATTGACACCCCTGGGCACGTTGACTTTACTATCGAAGTAGAG
CGTTCCATGCGTGTTCTTGACGGCGCGGTAATGGTTTACTGTGCAGTTGGTGGTGTTCAG
CCACAGTCTGAAACCGTATGGCGTCAGGCTAATAAATATAAAGTTCCACGTATTGCGTTC
GTTAACAAAATGGACCGTATGGGTGCGAACTTCCTGCGCGTAGTTGGTCAACTGAAATCT
CGCCTTGGTGCGAACCCAGTTCCACTGCAGTTGGCAATTGGCGCAGAAGAAAAATTCACC
GGTATTATCGATCTGGTGAAAATGAAAGCGATCAACTGGAACGAAGCTGATCAGGGCGTG
ACCTTCGAATATGAAGAAATCCCTGCTGATATGGCTGAACTGGCTGCTGAATGGCACCAG
AATCTGGTTGAATCTGCGGCAGAAGCGTCTGACGAGCTGATGGACAAATACTTGGGTGGC
GAAGAGCTGACCGAAGAAGAAATCAAGAAAGCTTTACGTCAACGTGTTCTGAAAAGCGAA
ATTATTCTTGTTACCTGTGGTTCTGCGTTTAAAAACAAAGGCGTACAGGCAATGCTGGAT
GCGGTTATTGAGTACCTGCCTGCACCAACTGACGTTGAATCAATCAACGGCATCTTGGAT
GATGGCAAAGATACTCCGGCTGTTCGTCATTCTGACGACAAAGAGCCGTTCTCTGCTCTG
GCGTTCAAAATCGCTACCGACCCATTCGTGGGTAACCTGACGTTCTTCCGCGTGTACTCT
GGTATTGTTAATTCCGGTGATACCGTTCTGAACTCAGTGAAATCGCAACGTGAACGCTTA
GGTCGTATCGTACAGATGCACGCTAACAAGCGTGAAGAGATCAAAGAAGTTCACGCCGGT
GATATCGCAGCCGCTATCGGTCTGAAAGATGTGACTACGGGTGACACTTTGTGTGACCCG
AATAATCCGATCATCTTGGAACGTATGGAGTTCCCAGAGCCGGTAATCTCTGTTGCTGTT
GAACCAAAAACCAAAGCTGACCAAGAAAAAATGGGTATGGCTCTGGGGCGTTTGGCGAAA
GAAGATCCATCATTCCGCGTTTGGACTGACGAAGAATCTGGTCAGACTATCATCGCTGGT
ATGGGTGAGTTGCATTTGGATATCCTGGTTGACCGTATGCGCCGCGAATTTAACGTGGAA
GCAAACGTCGGTAAACCTCAGGTTGCGTACCGTGAAACTATCCGCGAAACCGTTAAGGAT
GTGGAAGGTAAGCACGCTAAGCAGTCAGGCGGTCGTGGTCAGTATGGTCATGTTGTTATC
GACATGTCTCCATTGCCACCGGGTGGTGTTGGGTATGAATTCGTCAACGAAATCGTTGGT
GGTTCTATTCCTAAAGAATTCATTCCGGCCGTTGATAAAGGTATTCAAGAACAGCTGAAA
TCTGGCCCTCTGGCAGGTTACCCAGTTGTTGACGTTAAAGTGCGTCTGCACTACGGTTCT
TACCATGACGTTGACTCCTCAGAATTGGCATTTAAATTAGCTGGTTCTATCGCTTTTAAA
GAAGGTTTCAAACGAGCTAAACCAGTTCTGCTTGAGCCAATCATGAAGGTTGAAGTCGAA
ACCCCTGAAGATTACATGGGTGACGTAATGGGCGACCTGAACCGTCGTCGCGGTATCATC
GAAGGTATGGAAGATACTGCTACCGGTAAAACTGTTCGCGTCAAGGTTCCGTTGTCTGAA
ATGTTCGGTTATGCTACTGACCTGCGTTCTCAGACTCAGGGCCGTGCTTCTTACTCCATG
GAATTCTTGGAGTATGCTGAAGCACCGAGTAACGTCGCTAAAGCCGTTATCGAAGCCCGT
GGCAAATAG

Protein sequence predicted based on nucleotide sequence

MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERG
ITITSAATTCFWSGMAKQFEPHHVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQ
PQSETVWRQANKYKVPRIAFVNKMDRMGANFLRVVGQLKSRLGANPVPLQLAIGAEEKFT
GIIDLVKMKAINWNEADQGVTFEYEEIPADMAELAAEWHQNLVESAAEASDELMDKYLGG
EELTEEEIKKALRQRVLKSEIILVTCGSAFKNKGVQAMLDAVIEYLPAPTDVESINGILD
DGKDTPAVRHSDDKEPFSALAFKIATDPFVGNLTFFRVYSGIVNSGDTVLNSVKSQRERL
GRIVQMHANKREEIKEVHAGDIAAAIGLKDVTTGDTLCDPNNPIILERMEFPEPVISVAV
EPKTKADQEKMGMALGRLAKEDPSFRVWTDEESGQTIIAGMGELHLDILVDRMRREFNVE
ANVGKPQVAYRETIRETVKDVEGKHAKQSGGRGQYGHVVIDMSPLPPGGVGYEFVNEIVG
GSIPKEFIPAVDKGIQEQLKSGPLAGYPVVDVKVRLHYGSYHDVDSSELAFKLAGSIAFK
EGFKRAKPVLLEPIMKVEVETPEDYMGDVMGDLNRRRGIIEGMEDTATGKTVRVKVPLSE
MFGYATDLRSQTQGRASYSMEFLEYAEAPSNVAKAVIEARGK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2109
Mutations:
None
Discrepancy:
c285t
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable