clpA (Y. pestis KIM10+ )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  clpA
Gene Name:  ATP-binding component of serine protease
Original Clone ID:
FLH142043.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YpCD00016843
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2283nts
Open reading frame: 1 to 2283

ATGCCTATGCTCAATCAAGAACTTGAACTCAGTCTTAATATGGCTTTCGCCAGAGCGCGT
GAGCACAGACACGAGTTTATGACCGTGGAGCACCTGTTGCTGGCATTGCTGAGCAATCCA
GCCGCGCGGGAAGCGTTGGAGGCCTGTACGGTTGACCTGGTGGCGTTACGCCAGGAATTA
GAAGCGTTTATCGAACAAACTACACCGACACTACCTGTCAGTGAGGAAGAGCGTGATACC
CAGCCAACGCTCAGTTTTCAGCGCGTACTACAACGTGCGGTCTTTCATGTGCAGTCATCG
GGCCGTAATGAGGTTTCCGGCGCGAACGTGTTGGTCGCCATCTTCAGTGAACAAGAGTCT
CAGGCGGCCTACCTGTTGCGTAAGCATGATGTCAGCCGCTTGGATGTCGTGAACTTTATT
TCCCATGGTGCCCGTAAGGACGAGCCTAGCCAGACGCCGAATGTCGATAACTCGCCAAGT
GAAGAGCAGGCCAGCGGGGAAGACCGTATGGAAAACTTCACCACAAACCTAAATCAACTG
GCCCGTGTTGGCGGCATTGATCCGCTTATTGGGCGCGATAAAGAGCTGGAGCGGACCATT
CAGGTACTGTGCCGTCGGCGTAAAAACAACCCGCTGCTGGTGGGGGAATCCGGTGTTGGT
AAAACTGCCATTGCTGAGGGGCTGGCTTGGCGTATTGTGCAAGGTGATGTTCCTGACGTC
ATATCCGAATGCACGCTGTACTCATTGGATATCGGTTCATTGCTGGCGGGTACCAAATAC
CGTGGTGACTTTGAAAAACGGTTTAAGGCACTGCTAAAGCAGTTGGAAAACGACAAAGAC
AGCATCCTGTTTATCGATGAAATCCACACAATCATTGGCGCTGGTGCGGCTTCTGGTGGT
CAGGTGGATGCCGCGAATCTGATTAAACCTCTGCTTTCCAGTGGCAAGATCCGGGTTATT
GGTTCCACGACGTATCAGGAATTCAGTAATATCTTTGAGAAAGATCGCGCGCTGGCACGT
CGTTTCCAGAAAATTGATATCATTGAACCAACACCAGAAGAGACGGTTCAAATTATTAAT
GGTCTGAAACCCAAATATGAAGCGCACCACGATGTGCGTTACACCGCGAAAGCGATACGT
GCTGCGGTTGAATTGTCGGTTAAATATATTAATGACCGCCATTTGCCAGACAAAGCCATT
GATGTCATTGATGAAGCAGGTGCGCGTAGCCGCCTGATGCCAGTGAATAAACGCAAGAAA
ACGGTCAATGTCTCGGATATCGAATCGGTGGTAGCCCGTATTGCCCGTATCCCGGAGAAA
ACGGTTTCTGCCAGTGACCGCGATGTCCTACGAAATCTGAGTGATCGCCTAAACATGTTG
GTATTCGGTCAAGATAAGGCGATTGAGGCGTTATCTGAAGCGATCAAAATGAGCCGTGCG
GGGTTAGGGCATGAGCGTCAACCTGTCGGGTCCTTCTTGTTTGCTGGCCCTACGGGGGTA
GGTAAAACAGAAGTGACGGTGCAGTTGGCCAAAGCGCTCGATATCGAATTGCTCCGCTTT
GATATGTCTGAGTATATGGAGCGCCATACTGTCAGCCGCCTGATTGGTGCGCCCCCAGGG
TATGTTGGTTACGATCAGGGCGGGTTATTGACTGATTCAGTGATCAAACATCCTCATGCT
GTGTTGTTATTGGACGAAATTGAAAAAGCGCATCCTGATGTGTTTAATCTGTTATTGCAG
GTGATGGATAACGGAACACTGACTGATAACAATGGGCGTAAGGCCGATTTCCGTAACGTC
ATTTTGGTGATGACCACCAACGCCGGTGTACGGGAAACGCAGCGCACTTCCATTGGTTTT
AAACAGCAGGATAGCAGCACTGATGCGCTGGAAGAGATCAAAAAAATATTCACACCGGAG
TTCCGTAACCGGTTAGATAATATTATTTGGTTCAACCATTTGTCTCCTGCGGTTATCCAG
CAGGTGGTCGATAAATTTATCGTTGAGTTGCAGGCACAATTAGATGCGAAAGGCGTGTCA
TTGGAGGTCAGTGATGAAGCGCGCGATTGGTTATCTGAGAAGGGCTATGACAAAGCGATG
GGTGCTCGACCAATGACCCGGGTGATTCAGGAGAATCTGAAGAAACCACTGGCGAATGAA
TTGTTATTTGGTTCTCTGGTTGATGGTGGTTCGGTTACGGTTAATCTGGATAAAGAGAAA
CAACAACTGGCCTATGAATTCCAGAGCGCGCAAAAACGTAAAACTGAAGGGGCAGTTCAT
TAG

Protein sequence predicted based on nucleotide sequence

MPMLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPAAREALEACTVDLVALRQEL
EAFIEQTTPTLPVSEEERDTQPTLSFQRVLQRAVFHVQSSGRNEVSGANVLVAIFSEQES
QAAYLLRKHDVSRLDVVNFISHGARKDEPSQTPNVDNSPSEEQASGEDRMENFTTNLNQL
ARVGGIDPLIGRDKELERTIQVLCRRRKNNPLLVGESGVGKTAIAEGLAWRIVQGDVPDV
ISECTLYSLDIGSLLAGTKYRGDFEKRFKALLKQLENDKDSILFIDEIHTIIGAGAASGG
QVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKDRALARRFQKIDIIEPTPEETVQIIN
GLKPKYEAHHDVRYTAKAIRAAVELSVKYINDRHLPDKAIDVIDEAGARSRLMPVNKRKK
TVNVSDIESVVARIARIPEKTVSASDRDVLRNLSDRLNMLVFGQDKAIEALSEAIKMSRA
GLGHERQPVGSFLFAGPTGVGKTEVTVQLAKALDIELLRFDMSEYMERHTVSRLIGAPPG
YVGYDQGGLLTDSVIKHPHAVLLLDEIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNV
ILVMTTNAGVRETQRTSIGFKQQDSSTDALEEIKKIFTPEFRNRLDNIIWFNHLSPAVIQ
QVVDKFIVELQAQLDAKGVSLEVSDEARDWLSEKGYDKAMGARPMTRVIQENLKKPLANE
LLFGSLVDGGSVTVNLDKEKQQLAYEFQSAQKRKTEGAVH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2283
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable