rpoC (Y. pestis KIM10+ )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  rpoC
Gene Name:  RNA polymerase, beta prime subunit
Original Clone ID:
FLH143218.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
YpCD00017102
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4257nts
Open reading frame: 1 to 4257

ATGCCTATCAGGTCTAACTCCGACAGGAGCCAATCCGTGAAAGACTTATTAAAGTTTCTG
AAAGCGCAAACTAAGACCGAAGAGTTTGATGCGATCAAAATTGCTCTGGCATCGCCAGAC
ATGATCCGTTCATGGTCATTCGGTGAAGTTAAAAAGCCGGAAACCATTAACTACCGTACG
TTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGCCCAGTAAAAGATTAC
GAATGCCTGTGCGGTAAGTACAAGCGTTTAAAACACCGTGGTGTTATTTGTGAGAAATGC
GGCGTTGAAGTGACTCAAACTAAAGTGCGCCGTGAGCGTATGGGTCACATCGAACTGGCT
TCTCCAACTGCGCACATCTGGTTCCTGAAATCGCTGCCATCTCGTATCGGTTTGCTGCTG
GATATGCCATTACGTGACATCGAACGTGTACTGTACTTCGAATCCTATGTGGTTATTGAA
GGCGGTATGACCAACCTGGAACGTCGCCAGATTCTGACTGAAGAGCAGTATCTGGATGCG
TTGGAAGAGTTTGGTGATGAGTTCGACGCGAAAATGGGCGCCGAGGCTATTCAGGCCCTG
TTGAAAAACATGGATCTGGAAGCTGAGTGCGAGATCCTGCGTGAAGAATTGAACGAAACA
AATTCTGAAACCAAGCGTAAGAAACTGACCAAGCGTATCAAGTTGCTGGAAGCGTTCGTA
CAGTCTGGCAACAAGCCAGAGTGGATGATCCTGACCGTGCTGCCTGTGTTGCCGCCAGAT
CTGCGTCCATTGGTTCCGTTGGATGGTGGTCGCTTTGCGACTTCCGATCTGAACGATTTG
TATCGTCGCGTGATCAACCGTAACAACCGTCTGAAACGCCTGCTGGACTTGGCGGCACCG
GACATCATCGTACGTAACGAAAAGCGTATGCTGCAAGAAGCAGTAGATGCATTGCTGGAT
AACGGCCGTCGCGGTCGTGCGATTACCGGCTCTAACAAGCGTCCGTTGAAGTCACTGGCT
GACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTGGGTAAACGTGTTGAC
TACTCAGGTCGTTCGGTTATCACCGTTGGTCCATACCTGCGTCTGCATCAGTGCGGTCTG
CCTAAGAAAATGGCTCTTGAGCTGTTTAAACCGTTCATCTACGGCAAGCTGGAATTGCGT
GGTCTTGCTACCACCATCAAAGCCGCCAAGAAGATGGTTGAGCGTGAAGAAGCGGTTGTT
TGGGATATCCTGGATGAAGTTATCCGCGAACACCCAGTGCTGCTGAACCGTGCACCAACC
CTTCACCGTTTGGGTATCCAGGCGTTTGAACCGGTTCTGATCGAAGGTAAGGCAATCCAG
TTGCACCCATTGGTTTGTGCGGCATATAACACCGACTTCGATGGTGACCAAATGGCTGTC
CACGTACCGCTGACACTGGAAGCTCAGTTAGAAGCGCGTGCGTTAATGATGTCTACCAAC
AACATCCTGTCACCCGCGAACGGCGAGCCAATCATCGTTCCTTCTCAGGACGTTGTATTG
GGTCTGTACTACATGACCCGCGACTGTGTTAACGCCAAAGGCGAAGGCATGGTTCTGACT
GGCCCGAAAGAAGCTGAGCGTATTTATCGCGCTGGCTTGGCATCTCTGCATGCCCGCGTA
AAAGTACGTATTACCGAAGAAATTCGTAATACCGAAGGCGAAAGCATTACGCGTACCAGC
ATTATCGATACGACTGTAGGCCGTGCTATTTTGTGGATGATCGTCCCGCAAGGCCTGCCT
TACTCTATCGTTAACCAGCCGCTGGGTAAAAAAGCTATCTCTAAGATGCTGAACACGTGT
TACCGCATCTTGGGCTTGAAGCCAACGGTTATCTTTGCTGACCAGATCATGTACACCGGT
TTTGCTTACGCAGCACGCTCAGGCGCCTCTGTAGGTATCGATGACATGGTTATCCCAGAA
GCGAAAGCGGGGATCATCGAAGAAGCTGAAACTGAAGTTGCTGAGATCCAAGAACAGTTC
CAATCTGGTCTGGTCACGGCAGGCGAACGTTATAACAAAGTGATCGATATCTGGGCTGCG
GCTAACGAACGTGTTGCTAAAGCGATGATGGATAACTTGTCGGTTGAGGATGTTGTCAAC
CGTGATGGCGTTGTTGAACAGCAAGTTTCATTCAACAGCATCTTCATGATGGCCGACTCC
GGTGCGCGTGGTTCTGCCGCACAGATTCGTCAGCTGGCAGGTATGCGTGGTCTGATGGCT
AAGCCAGACGGCTCGATCATTGAAACGCCAATCACCGCGAACTTCCGTGAAGGTTTGAAC
GTACTTCAGTACTTCATCTCAACCCACGGTGCGCGTAAAGGTTTGGCGGATACCGCATTG
AAAACTGCGAACTCCGGTTACCTGACCCGTCGTTTAGTTGACGTGGCACAGGATCTGGTG
GTGACTGAGGATGATTGTGGTACCCATAACGGTATCGTGATGACCCCGGTTATCGAAGGT
GGTGATGTTAAAGAGCCACTGCGTGATCGCGTACTGGGTCGTGTGACTGCAGAAGAAGTT
ATCAAGCCAGGTTCGGCTGATATTCTGGTTCCGCGTAACACCCTGCTGGACGAAAAATGG
TGTGATCTGTTAGAAGAAAACTCCGTCGACAGCGTTAAAGTTCGCTCCGTAGTAAGCTGT
GAAACTGATTTCGGTGTGTGTGCTAACTGCTATGGTCGCGACCTGGCACGTGGCCACATC
ATCAACAAAGGTGAAGCGGTTGGTGTTATTGCAGCACAGTCCATCGGTGAGCCGGGTACT
CAGCTTACGATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCAGCAGAATCC
AGCATCCAGGTTAAGAACAAAGGCAGCTTGAAACTGAGCAACGTCAAGTTCGTGACCAAT
GCGGCTGGCAAGCTGGTTATTACGTCTCGTAATACCGAGCTGAAGCTGATCGACGAATTC
GGTCGTACCAAAGAAAGCTATAAAGTGCCTTACGGTGCGGTAATGGCTAAAGGCGATGGC
GCAGAAGTTCAGGGCGGCGAAACCGTTGCTAACTGGGATCCGCACATCATGCCAGTGGTC
ACCGAAGTGAGCGGCTTCATTCGCTTCGCCGACATGGTTGATGGCCAGACCATTACTCGC
CAGACCGACGAACTGACTGGTTTGTCTTCTCTGGTCGTTCTGGACAGTGCAGAGCGTACG
GGTAGCGGTAAAGACCTGCGTCCAGCACTGAAAATCGTTGATGCCAAAGGCAATGACGTA
TTGATCCCAGGTACTGATATGCCTGCTCAATACTTCCTGCCAGGTAAAGCGATTGTACAG
CTGGAAGACGGTATTCAGATCGGTGCGGGTGATACCTTGGCGCGTATTCCTCAGGAATCA
AGCGGTACCAAGGATATTACCGGTGGTCTGCCACGTGTTGCTGACTTGTTCGAAGCACGT
CGTCCGAAAGAGCCTGCAATCCTGGCTGAAATCAGCGGGATCATCTCGTTCGGTAAAGAG
ACCAAAGGCAAGCGTCGTCTGGTGATCTCACCGTTGGATGGTAGCGATGCATACGAAGAG
ATGATTCCGAAATGGCGTCAGCTTAACGTGTTCGAAGGTGAAGTTGTGGAACGCGGTGAC
GTGGTTTCCGATGGCCCAGAATCTCCACATGACATTCTGCGTCTGCGTGGCGTTCACGCG
GTTACCCGCTACATCACCAACGAAGTGCAGGAAGTTTACCGTCTGCAAGGCGTTAAGATT
AACGATAAACACATTGAAGTTATCGTTCGTCAGATGCTGCGTAAAGGCACTATCGTTGAT
GCAGGTAGCACCGACTTCCTGGAAGGCGAGCAGGCAGAAATGTCCCGCGTTAAAATCGCT
AACCGTAAGCTGGCAGCAGAAGGCAAAATTGAGGCTACGTTCACTCGTGACCTGCTGGGT
ATCACCAAGGCATCCTTGGCGACCGAGTCCTTCATCTCTGCGGCTTCGTTCCAGGAAACC
ACGCGCGTTCTTACTGAAGCGGCAGTTGCCGGTAAACGTGATGAATTGCGTGGCCTGAAA
GAAAACGTAATCGTGGGCCGTCTGATCCCAGCCGGTACCGGTTATGCTTATCATCAGGAC
CGTATGCGCCGTAAAGCACAGGGTGAAGCGCCTGTTGTTCCACAAGTCAGCGCGGATGAA
GCGACTGCAAACTTGGCCGAATTGCTTAATGCCGGTTTTGGTAACAACAAAGGCTAG

Protein sequence predicted based on nucleotide sequence

MPIRSNSDRSQSVKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRT
FKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELA
SPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERRQILTEEQYLDA
LEEFGDEFDAKMGAEAIQALLKNMDLEAECEILREELNETNSETKRKKLTKRIKLLEAFV
QSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAP
DIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVD
YSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVV
WDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNTDFDGDQMAV
HVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLT
GPKEAERIYRAGLASLHARVKVRITEEIRNTEGESITRTSIIDTTVGRAILWMIVPQGLP
YSIVNQPLGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPE
AKAGIIEEAETEVAEIQEQFQSGLVTAGERYNKVIDIWAAANERVAKAMMDNLSVEDVVN
RDGVVEQQVSFNSIFMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLN
VLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHNGIVMTPVIEG
GDVKEPLRDRVLGRVTAEEVIKPGSADILVPRNTLLDEKWCDLLEENSVDSVKVRSVVSC
ETDFGVCANCYGRDLARGHIINKGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAES
SIQVKNKGSLKLSNVKFVTNAAGKLVITSRNTELKLIDEFGRTKESYKVPYGAVMAKGDG
AEVQGGETVANWDPHIMPVVTEVSGFIRFADMVDGQTITRQTDELTGLSSLVVLDSAERT
GSGKDLRPALKIVDAKGNDVLIPGTDMPAQYFLPGKAIVQLEDGIQIGAGDTLARIPQES
SGTKDITGGLPRVADLFEARRPKEPAILAEISGIISFGKETKGKRRLVISPLDGSDAYEE
MIPKWRQLNVFEGEVVERGDVVSDGPESPHDILRLRGVHAVTRYITNEVQEVYRLQGVKI
NDKHIEVIVRQMLRKGTIVDAGSTDFLEGEQAEMSRVKIANRKLAAEGKIEATFTRDLLG
ITKASLATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQD
RMRRKAQGEAPVVPQVSADEATANLAELLNAGFGNNKG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4257
Mutations:
None
Discrepancy:
g1411a,A471T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable