dxs (Y. pestis KIM10+ ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  dxs
Gene Name:  1-deoxyxylulose-5-phosphate synthase
Original Clone ID:
FLH149464.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YpCD00017170
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1860nts
Open reading frame: 1 to 1860

ATGAGTCTTGATATAGCCAAATACCCGACATTGGCACTAGCGGAGAACCCAGAAGAACTG
CGCATGCTGCCAAAAGAAAGCTTGCCGAAGTTATGTGATGAACTCCGGCAGTATTTGCTC
ACCTGTGTCAGCCGATCCAGCGGGCATTTTGCTTCCGGGTTAGGCGTAGTCGAACTGACT
GTCGCTTTACATTACGTTTATAATACGCCGTTTGATCATTTGATATGGGACGTAGGTCAT
CAGGCTTATCCGCACAAAATATTGACCGGCCGCCGTGATCGAATCTCTACTATTCGTCAA
AAAGATGGTTTGCACCCTTTTCCATGGCGCGGAGAGAGTGAATACGACGTGCTTTCTGTT
GGTCACTCCTCTACATCGATCAGCGCGGGTCTGGGTATGGCGGTTGCTGCCGAGCGGGAA
GGTAAAGGGCGACGTACCGTTTGCGTTATCGGTGATGGTGCTATCACCGCAGGTATGGCT
TTTGAAGCAATGAGTCATGCCGGAGATATTCACTCAGACATGCTGGTTATCCTCAATGAT
AATGGAATGTCTATTTCAGAAAATGTAGGTGGCCTGAACAACCATTTGGCTCAGCTATTA
TCTGGAAAACTCTATGCCAGCCTACGTGAAGGCGGTAAAAAAGCATTTTCTGCCTTACCA
CCAATCAAAGATCTGCTGAAACGAACCGAAGAGCATCTGAAAGGGATGGTAGTCCCCAGT
ACGTTGTTTGAAGAGTTAGGTTTTAATTATATTGGTCCCGTTGATGGGCATGATGTACAC
ACGTTGACGCAAACGCTAAAAAATATGCGCGACCTGAAAAGTCCACAACTTCTGCACATC
ATGACTAAAAAGGGCAAAGGCTATGCTCCGGCAGAAAAGGATCCAATCGGTTGGCATGCC
GTACCTAAGTTTGATCCCGCCTCCGGCACCTTGCCTAAAAGCCAGAGCAGCCTCCCCACT
TACTCCAAAATATTTGGCGAATGGTTATGCGAAACCGCAGCCAAAGATAGCAAATTAATG
GCAGTGACACCGGCGATGCGTGAAGGATCCGGCATGGTGCGTTTCTCGCGCGAATATCCG
CAACAATATTTCGATGTCGCCATCGCTGAACAACATGCAGTGACCTTTGCCGCAGGCCTG
GCTATTGGTGGCTACAAGCCTGTGGTTGCCATCTATTCGACTTTCTTGCAACGCGCCTAT
GATCAGTTGATCCATGATGTTGCTATCCAAAATTTGCCCGTGCTCTTTGCCATTGACCGT
GGCGGTTTGGTCGGTGCTGATGGGCAGACTCACCAAGGTGCATTCGATCTTTCATTTATG
CGCTGTATTCCAAACATGGTCATTATGGCACCAAGTGATGAGAATGAGTGCCGTCAGATG
CTATACACCGGCTATCACCATAATGGACCTGCCGCAGTACGTTACCCACGAGGTAATGGT
ACGAGCGCGGTGCTGGAGCCATTGGAGATGTTGCCGATCGGCAAAGGGGTACTACGCCGT
GAAGGTGAGAAGATTGCCATCCTTTGCTTTGGTACGTTACTTGCTCAGGCACAGCTTGCC
GCAGAAAACCTCAATGCCACGCTGGTGGATATGCGTTTCGTGAAACCGCTAGATGAAGAA
TTGGTATTGGAAATGGCAGCAAAACATCAGGTATTAGTGACTGTCGAAGAAAACGCCATC
ATGGGGGGGGCGGGTAGCGGTGTGAATGAGCTATTAATGGCAAAACGGCGATGGGTCCCA
GTACTCAATATTGGTTTACCCGATCTATTTGTGCCACAAGGTGAGCAAGACGAAATGCGA
TCTGAACTCGGTTTAGATGCGGCGGGTATACAGCGTCAAATTGAAGCTTGGCTAGCTTAG

Protein sequence predicted based on nucleotide sequence

MSLDIAKYPTLALAENPEELRMLPKESLPKLCDELRQYLLTCVSRSSGHFASGLGVVELT
VALHYVYNTPFDHLIWDVGHQAYPHKILTGRRDRISTIRQKDGLHPFPWRGESEYDVLSV
GHSSTSISAGLGMAVAAEREGKGRRTVCVIGDGAITAGMAFEAMSHAGDIHSDMLVILND
NGMSISENVGGLNNHLAQLLSGKLYASLREGGKKAFSALPPIKDLLKRTEEHLKGMVVPS
TLFEELGFNYIGPVDGHDVHTLTQTLKNMRDLKSPQLLHIMTKKGKGYAPAEKDPIGWHA
VPKFDPASGTLPKSQSSLPTYSKIFGEWLCETAAKDSKLMAVTPAMREGSGMVRFSREYP
QQYFDVAIAEQHAVTFAAGLAIGGYKPVVAIYSTFLQRAYDQLIHDVAIQNLPVLFAIDR
GGLVGADGQTHQGAFDLSFMRCIPNMVIMAPSDENECRQMLYTGYHHNGPAAVRYPRGNG
TSAVLEPLEMLPIGKGVLRREGEKIAILCFGTLLAQAQLAAENLNATLVDMRFVKPLDEE
LVLEMAAKHQVLVTVEENAIMGGAGSGVNELLMAKRRWVPVLNIGLPDLFVPQGEQDEMR
SELGLDAAGIQRQIEAWLA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1860
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable