Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH174673.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
VcCD00017452
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2712nts
Open reading frame: 1 to 2712
ATGATAACTAAGCTACTGACAAAGGTGATTGGCAGTCGCAATGACCGCACACTGCGCCGT
TTACGCAAGATTGTAAAAGAGATTAATAACTACGAACCCGCCTTTGAAGCCCTCTCCGAT
GAAGAGCTAAAAGCGAAAACTGTCGAGTTCCGTCAACGCATTGAACAGGGCGAAAACCTT
GATCAACTGTTGCCAGAAGCTTTTGCCACCGTGCGCGAAGCTTCTAAGCGTGTGTTCGGC
ATGCGCCATTTTGATGTACAGCTGATTGGCGGTATGGTGCTGCATGGCGGTCAAATCGCT
GAGATGCGTACGGGTGAAGGTAAAACGCTAACCGCAACGCTGGCTGCTTACCTGAATGCC
CTACCTGGCAAAGGCGTACACATTGTTACGGTCAACGACTACTTGGCCAAACGTGACGCA
GAAACCAACCGACCACTCTTCGAATTCTTAGGCATGACCGTGGGTGTGAATATTCCCAAC
ATGCCGCAGCCTGCTAAAAAAGAGGCTTACCAAGCCGACATCCTGTACGGCACCAACAAC
GAATTTGGTTTTGACTACCTGCGCGATAACATGGCGTTTCGCCCTGAAGATCGCGTGCAA
CGTGCTCGCTTCTTTGCTGTGGTCGATGAAGTGGACTCAATCTTAATCGATGAAGCACGT
ACCCCGCTGATTATCTCAGGTCCTGCGGAAGACAGTTCGGATCTCTACATCCGTATCAAC
AAGTTGATCCCGCTACTGCAAAAGCAAGATAAAGAAGATTCGGAAGAGTACCGCGGTGAC
GGTCACTTCACCGTGGATGAAAAATCTAAGCAGGTGCATCTGACCGAAACGGGTCAAGAG
TTCGTTGAAGAGCTGCTAGTGAAAAACGGCATGATGCAAGAGGGTGATACGCTTTACTCT
CCCGCTAACATTAGCTTGCTGCACCACGTTAACGCGGCATTACGTGCGCATGTTCTGTTT
GAAAAGAACGTCGATTACATCGTGACACCCGATGGTGAAGTGGTGATCGTTGATGAACAT
ACCGGTCGTACTATGCCGGGTCGTCGCTGGTCTGATGGTCTGCACCAAGCGGTAGAAGCA
AAAGAAGGGGTGAAGATCCAGAACGAAAACCAAACTCTGGCTTCGATCACTTTCCAAAAC
TACTTCCGTCTGTATGAAAAGCTGTCTGGTATGACGGGTACCGCTGATACCGAAGCGTTT
GAATTCCAACAAATTTATGGTTTGGAAACAGTTGTTATTCCAACCAACAAGCCGATGGTG
CGTAACGATATGCCGGATGTGGTGTATCGCTCTGAGGCGGAAAAGTTTGCCGCGATCATT
GAAGATATCAAACAGCGTGTTGAGAAAGGTCAACCTGTGTTGGTGGGTACGGTTTCGATT
GAGAAATCAGAATTGCTCTCCAATGCACTGAAAAAAGCAGGCATCAAGCACAATGTTCTG
AACGCGAAGTTCCATGAAAAAGAAGCGGAAATTGTTGCTGAAGCCGGTAAACCTGGCGCA
GTCACCATCGCAACTAACATGGCGGGTCGTGGTACGGATATCGTATTGGGTGGCAGCTGG
CAAGCCAAAGTGGAGAAGCTGGACAACCCAACCCAAGAGCAAATTGATGCGATTAAAGCT
GAGTGGAAGCAAGTGCACGATCAAGTGTTGCAAGCGGGCGGCCTACACATCATTGGTACT
GAGCGTCACGAATCTCGCCGTATCGATAACCAGCTACGTGGTCGTTCTGGTCGTCAAGGT
GATGCGGGTTCATCGCGTTTCTATCTCTCAATGGAAGATACTTTACTACGTATTTTCACC
TCTGATCGTATGGCGGCATTGATCCAAAGCGGTATGGATGAAGGCGAAGCGATTGAGTCA
AAAATGCTCTCTCGCTCGATTGAAAAAGCGCAGCGTAAAGTAGAAGGCCGTAACTTCGAC
ATTCGTAAACAGTTGCTGGAATACGATGACGTAGCGAACGATCAGCGTAAAGTCGTTTAC
GAACTGCGTGATGAACTGATGAGCGCAGATGACATCAGCGACATGATCGCGCAAAACCGT
GAAGATGTGCTGAACGCGGTGATGGATGAGTACATTCCGCCACAATCTCTGGAAGATATG
TGGGATATCAAAGGGTTGGAAGATCGCCTAAAGAACGATTTCGATCTACCACTACCGATC
CAATCTTGGTTAGATGCTGACAACAAGCTGTATGAAGAAGCGCTACGTGAGCGCATCATC
GAGCAAGCGGTTGAGGTTTATAAAGCCAAAGAGCAAGCGGTTAGCCCTGCGGTGATGCGT
AACTTTGAAAAATCTGTGATGCTGCAAACGCTAGATACACTGTGGAAAGAGCATCTGGCG
GCGATGGATCACTTGCGTCAAGGTATTCATCTACGTGGTTACGCACAGAAAAACCCGAAA
CAAGAGTACAAGCGCGAGTCGTTTGAACTGTTTGAAGATCTGCTTGAGTCACTCAAATCG
GATGTGATCACTGTCTTGTCCAAAGTCCGTGTTCAGCAGCAAGAAGAAGTGGAGCGTATG
GAAGCTCAACGCCGCGCGCAAGCTGAAGAAGCGGCGCGTCATGCACAAGCGCAACACGCG
AGTGCTGATGATGCTGAGCAAGACGAGTCGAACCAGCCAATGGTGCGTGATGAACGCAAA
GTGGGCCGTAACGAACCTTGTCCATGCGGTAGCGGTAAAAAATACAAACAGTGTCACGGG
CAAATTAACTTG
Protein sequence predicted based on nucleotide sequence
MITKLLTKVIGSRNDRTLRRLRKIVKEINNYEPAFEALSDEELKAKTVEFRQRIEQGENL DQLLPEAFATVREASKRVFGMRHFDVQLIGGMVLHGGQIAEMRTGEGKTLTATLAAYLNA LPGKGVHIVTVNDYLAKRDAETNRPLFEFLGMTVGVNIPNMPQPAKKEAYQADILYGTNN EFGFDYLRDNMAFRPEDRVQRARFFAVVDEVDSILIDEARTPLIISGPAEDSSDLYIRIN KLIPLLQKQDKEDSEEYRGDGHFTVDEKSKQVHLTETGQEFVEELLVKNGMMQEGDTLYS PANISLLHHVNAALRAHVLFEKNVDYIVTPDGEVVIVDEHTGRTMPGRRWSDGLHQAVEA KEGVKIQNENQTLASITFQNYFRLYEKLSGMTGTADTEAFEFQQIYGLETVVIPTNKPMV RNDMPDVVYRSEAEKFAAIIEDIKQRVEKGQPVLVGTVSIEKSELLSNALKKAGIKHNVL NAKFHEKEAEIVAEAGKPGAVTIATNMAGRGTDIVLGGSWQAKVEKLDNPTQEQIDAIKA EWKQVHDQVLQAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDTLLRIFT SDRMAALIQSGMDEGEAIESKMLSRSIEKAQRKVEGRNFDIRKQLLEYDDVANDQRKVVY ELRDELMSADDISDMIAQNREDVLNAVMDEYIPPQSLEDMWDIKGLEDRLKNDFDLPLPI QSWLDADNKLYEEALRERIIEQAVEVYKAKEQAVSPAVMRNFEKSVMLQTLDTLWKEHLA AMDHLRQGIHLRGYAQKNPKQEYKRESFELFEDLLESLKSDVITVLSKVRVQQQEEVERM EAQRRAQAEEAARHAQAQHASADDAEQDESNQPMVRDERKVGRNEPCPCGSGKKYKQCHG QINL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2712
Mutations:
None
Discrepancy:
g1788a
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
