Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH174705.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
VcCD00017459
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2883nts
Open reading frame: 1 to 2883

ATGTTCAAAGAGAACAAAAATAAAGTCGAAACCATTATTAAAACGAGTGAAGAGGCTCCT
TCTTCTCGACTCAATGGCTCACAACGTCTTAAAGAAAGCGGATTGATCTTAGCTTTCTTG
TTTTCGATATTTCTTGCCGTTGCTCTGTTTAGTTTTAACCCCGCTGATCCATCTTGGTCA
CAAACGGCTTGGGGGACAGACATTCACAATGCGGGTGGTTTAGTTGGCGCTTGGTTAGCT
GACACCCTGTTCTTCGTTTTTGGCTCTTTAGCCTATCCATTGCCTTTTATTACTGCATTT
GCAGCATGGGTGCTGTTGCGCAAACGTGATGAAGGTGACGAAATCGATTTCACTCTTTGG
GGAACTCGACTCCTTGGTCTAACCATAGTGCTATTGACCAGTTGTGGTCTTGCCGACATC
AACTTTGATGACATTTGGTATTTTTCATCAGGTGGAGTGATTGGCGATGTGTTGACCAGC
TTAGCACTGCCGACCTTGAACATTCTTGGAACAACGCTTGTGTTGCTCTTTTTGTGGGGG
GCTGGCATTACCTTGTTGACTGGGATCTCTTGGCTGCGGATTGTGGAATGGATTGGTGAG
CGTAGCATTGCGGCATTTGTCGGGCTGTTTAACCGCTTACGCGGTGAAAAAGCGGAGCGT
GTGAAACCGGCACTGGTAAAGCCTGAGCTGCCAGTTGAAGAGCTAGAACCGACATTTTCT
GCGTCAATGGATACTGAGATTGATGAGCCAGCTCCGAGCTTACGTCGCTTCAATATTCAT
ATGCCGGAAGAGCGCGACGTACCTGACATTCATTTTGAGCCCCAAGTGGAGCCTAAAGTC
GAGTTGAAACCAGAGCCGCCAAGGCAGCGCGAACCTGCTCCCCATTTCTCACGGGTAGCC
GCTCAAAATACACAGGTAGAGCCTGTCAGCTCTGCGCGCACTCAGCAATGGGATGCCACG
ATAGAAGAACTGGAGCAACAGGCTCGTCTAGTCGATGACTATGCTGTCGAGGATGATGCA
GTGCCAAGCGTTCTTACCTCCAGCACTCTTTCGGATGTTGAAGATTCCATTTTGACCACT
GCCATCTCGGTGGATGAAGAAGAGGAGAGCCTCTCTGAAAACTTTAACCACTCGTTCAAT
ATTGAGGTAGAAGATGAAGAAGTTGAGCCCAGCATTGCTAACTTGCATTGGTCAGATGAT
GAAGATGAGCTTGAAGAAACGCCTTCGGTTATGGTATCGCCCGCAATAGAGTCTGATTGG
GAAGACGAGGATGAGCCTGACGATCGCGATGTGGCGGCATTTCAAAACATCGTTTCGCAA
GCACAAGCGAATGCGGCTGCGCAGCAAAACCCGTTTTTAGTGCAGAAGGCCGTAAACTTG
CCGAAACCAACAGAGCCTATGCCAACGCTAGAACTGCTGTATCATCCAGAAAAGCGTGAA
AACTTTATCGATCGTGAAGCTTTAGAAGAGATCGCGCGTCTGGTTGAGTCCAAACTGGCT
GATTACAAAATCCAAGCGCAAGTCGTGGATATTTTCCCGGGGCCTGTGATCACCCGTTTT
GAGCTAGATTTAGCGCCGGGTGTGAAAGTGAGCCGAATTTCTAGTCTGTCGATGGACTTA
GCCCGTTCACTCTCGGCGATGGCTGTGCGTGTCGTGGAAGTTATTCCCGGTAAACCTTAT
GTCGGGCTTGAGCTGCCAAACATGTCACGTCAAACTGTGTACCTATCCGATGTGATTGCC
AGTCCTCAATTTAAAGAGTCGAAGTCACCGACGACCGTCGTGCTTGGCCAAGATATCGCC
GGCGATGCCGTGGTAGCAGATCTGTCTAAGATGCCTCATGTGTTGGTGGCGGGTACCACT
GGTTCAGGTAAGTCGGTTGGGGTCAACGTGATGATCCTGAGCATGTTGTATAAGGCTTCC
CCAGAAGATGTCCGCTTCATCATGATTGACCCGAAAATGTTAGAGCTTTCCGTGTATGAA
GGCATTCCTCATCTGTTGGCGGAAGTGGTGACCGACATGAAAGATGCCTCTAACGCGCTG
CGCTGGTGTGTCGGAGAAATGGAGCGTCGTTATAAGCTGATGTCGGTACTTGGTGTACGT
AATATCAAAGGCTTTAACGATAAACTGCGTATGGCTGCTGAAGCCGGACATCCTATCTAC
GATCCTCTTTGGAAAGACGGAGATAGCATGGAGAGTGAGCCGCCACTATTAGAAAAACTG
CCTTATATTGTGGTCGTGGTCGATGAATTTGCTGACCTGATGATGGTAGTCGGTAAAAAA
GTCGAAGAGCTGATTGCGCGTTTGGCACAAAAAGCCCGTGCGGCGGGTATTCACTTGATT
TTGGCTACGCAGCGCCCATCTGTGGATGTGATTACGGGTCTGATTAAAGCCAACATCCCG
ACCCGTGTTGCTTTTACTGTCTCTACGAAAACCGACTCTCGGACGATTTTGGATCAGAGT
GGCGCAGAGTCGTTGCTCGGTATGGGGGATATGCTCTATTTACCTGCCGGTTCCAGTCAT
ACGATTCGTGTACATGGTGCATTTGCCTCGGATGATGATGTGCATGCTGTGGTCAATAAC
TGGAAGGCAAGGGGCAAGCCGAATTACATCAGTGAGATTATCCAAGGCGATCACGGCCCA
GAAGCCTTATTGCCCGGAGAGCAGTCTGAATCAGACGAAGAACTTGACCCACTGTTTGAT
CAAGTGGTTGAGCACGTTGTCGAAACGCGCCGAGGCTCAGTTTCTGGGGTTCAACGCCGT
TTTAAAATTGGTTATAACCGCGCAGCACGTATTGTCGAGCAGCTTGAGGCTCAAGGCATT
GTGAGTGCGCCAGGGCATAACGGTAATCGTGATGTATTGGCTCCTGCACCTATACGAGAT
TTG

Protein sequence predicted based on nucleotide sequence

MFKENKNKVETIIKTSEEAPSSRLNGSQRLKESGLILAFLFSIFLAVALFSFNPADPSWS
QTAWGTDIHNAGGLVGAWLADTLFFVFGSLAYPLPFITAFAAWVLLRKRDEGDEIDFTLW
GTRLLGLTIVLLTSCGLADINFDDIWYFSSGGVIGDVLTSLALPTLNILGTTLVLLFLWG
AGITLLTGISWLRIVEWIGERSIAAFVGLFNRLRGEKAERVKPALVKPELPVEELEPTFS
ASMDTEIDEPAPSLRRFNIHMPEERDVPDIHFEPQVEPKVELKPEPPRQREPAPHFSRVA
AQNTQVEPVSSARTQQWDATIEELEQQARLVDDYAVEDDAVPSVLTSSTLSDVEDSILTT
AISVDEEEESLSENFNHSFNIEVEDEEVEPSIANLHWSDDEDELEETPSVMVSPAIESDW
EDEDEPDDRDVAAFQNIVSQAQANAAAQQNPFLVQKAVNLPKPTEPMPTLELLYHPEKRE
NFIDREALEEIARLVESKLADYKIQAQVVDIFPGPVITRFELDLAPGVKVSRISSLSMDL
ARSLSAMAVRVVEVIPGKPYVGLELPNMSRQTVYLSDVIASPQFKESKSPTTVVLGQDIA
GDAVVADLSKMPHVLVAGTTGSGKSVGVNVMILSMLYKASPEDVRFIMIDPKMLELSVYE
GIPHLLAEVVTDMKDASNALRWCVGEMERRYKLMSVLGVRNIKGFNDKLRMAAEAGHPIY
DPLWKDGDSMESEPPLLEKLPYIVVVVDEFADLMMVVGKKVEELIARLAQKARAAGIHLI
LATQRPSVDVITGLIKANIPTRVAFTVSTKTDSRTILDQSGAESLLGMGDMLYLPAGSSH
TIRVHGAFASDDDVHAVVNNWKARGKPNYISEIIQGDHGPEALLPGEQSESDEELDPLFD
QVVEHVVETRRGSVSGVQRRFKIGYNRAARIVEQLEAQGIVSAPGHNGNRDVLAPAPIRD
L

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2883
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable