polB (V. cholerae O1 biovar El Tor str. N16961 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH200559.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
VcCD00019983
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2364nts
Open reading frame: 1 to 2364

ATGAAGATTGAACAAGGGTTTGTATTAACGCGTCACGCTCGTGATGTGGCTGGACAAACC
CAAATTGAACTTTGGCTCGCGACACCTTCAGGCCCAACGCAACTTACCATACGCGGTGAG
CGTCCGGTTTTTTTTATTGAACAATCGGCTAGCCAAGAAGTGATGCGAATTGCCGCAGAA
TTATCAATAACGCCCTCATTAGCGCCTCTGTCATTACGTAGCTTTGCAGGCCAACCCCTC
GCCGCTTGTTACTGCAATACGATTCGCGATAGCCAAATCTTAGCCGAGAAACTCGCTCAA
GCGGACATGTTGATCTTAGAGGCGGATATTCGTTTGGCTGACCGTTTTCTGATGGAACGC
TTTATCCAAGGCAGCATCGAATTTACTGGTCAGATCACCGATTTCGGCCATTATCGCCAA
GTTCAGCAAGCCAAATGTCGCCAAGGTGATTACCTACCGACGCTGAATATGGTGTCGCTA
GACATCGAATGCTCCGAAAAAGGGCTACTCTACTCGATTGGTTTGGATAGCCCGATGGAC
AGCCGAGTGATTATGATAGGTCAACCAGAACCCGCGGAAACGCCGATTCAATGGGTCGAG
GATGAATACCAGTTACTCAAAGCGCTCATTGCATGGTTTGAGCAGTTTGATCCTGATGTG
ATTATTGGTTGGAGTGTAGTGGATTTCGATTTCCGGCTGCTGCACAAACGCGCGGAGTTC
CACAAACTGAAACTCACCATTGGCCGAGCTCAGCAACCGAGCTTTTTCCGTACGGCGAGC
CAAACGCAGCAGGGTTTTATCAGTATTCCTGGGCGTGTGGTGCTCGATGGTATCGATACG
TTAAAAACCGCGACTTATCATTTTCGTTCATGGTCTTTGGAGTCGGTCTCGCAAGAGCTG
CTCGGCGAAGGGAAAGCGATTCATAACGTGCATGATCGGATGGATGAAATTAACCAGATG
TTTCGCCACGATAAGCCCTCACTCGCTCGTTACAACCTGCAAGACTGCGTATTAGTGAAT
AAGATTTTTGCGGCCACTCATCTGCTCGATTTCGCCATTCAGCGTTCACGTTTAACGGGT
GTCGAGTTAGATCGCATTGGTGGCTCCGTGGCCGCATTCACCAATCTCTATCTACCACAA
CTGCACCGCGCAGGGTATGTCGCTCCCAATTTGCAGCCAGAAAACTGGGTTGCCAGCCCG
GGCGGTTATGTGATGGATTCGATTCCAGGGCTGTATGACTCGGTACTCGTGCTCGACTTT
AAAAGCCTCTACCCTTCGATTATTCGCTCGTTTTTGATTGATCCACTTGGGCTGATTGAA
GGATTAAAACTGCCAATAGGCAAACAGGCCGATCACGCGGTTCCGGGCTTTCGCGGCGGT
CAATTTCATCGCACCAAACACTTTTTACCTGAGATGATCGAAAAACTATGGGCAGCGCGC
GATGAAGCCAAACGCAACCAAGAAAAGGCTTTTTCACAAGCAATCAAAATCATCATGAAC
TCATTTTATGGTGTGCTCGGCTCATCAGGCTGCCGCTTTTTCGATGCGCGATTGGCATCG
AGCATTACCATGCGCGGCCATGAAATCATGATCCAAACAAAACGGTTGATTGAAGCGCGC
GGTTATCAAGTGATCTATGGCGATACGGATTCAACGTTTGTCGCACTCGGTGGGCAATAC
TCTCAGCAAGAAGCGGATAACATTGGTCACGCTTTGGTCCGAGAAATCAACCAATGGTGG
ACAGAGCATTTAAAGCAAGAATATGCCCTTACTTCGATTCTTGAACTCGAGTACGAAACC
CACTATCGCCGTTTTTTAATGCCGACCATTCGCGGTTCGGAAACGGGCTCCAAAAAACGC
TATGCGGGTTTAAAAGGGGATGGTGACAATGAGCAACTGATTTTTAAAGGTTTGGAAAGC
GCACGCACCGACTGGACTCCACTCGCGCAACGGTTTCAACATCAGCTGTATCAATTGATT
TTCCACGGTCAAGACCCAGAATCCTATATTCGCACTATCGTAGAACAGACATTGGCAGGT
CAATTGGATGATCAATTGGTATATCAAAAGCGTTTACGCCGACGGTTACATGAGTACCAA
AAGAATGTGCCGCCGCAAGTACGCGCAGCCAGAATGGCGGATGACATTAACGCCAAACTT
GGGCGACCGTTACAATACCAATACCGCGGAACGATTGAATATGTGATTACAGTCAATGGT
CCTGAGCCGAAAGAGTATTCCAAAAGCCCTATCGATTATCAGCACTACATCGATAAACAG
CTTAAACCGGTCGCAGATGCGATTTTGCCTTTTATTGGCAAACAATTTGATGAGCTTATT
GCGCCTCAACTGGGACTGTTTTTG

Protein sequence predicted based on nucleotide sequence

MKIEQGFVLTRHARDVAGQTQIELWLATPSGPTQLTIRGERPVFFIEQSASQEVMRIAAE
LSITPSLAPLSLRSFAGQPLAACYCNTIRDSQILAEKLAQADMLILEADIRLADRFLMER
FIQGSIEFTGQITDFGHYRQVQQAKCRQGDYLPTLNMVSLDIECSEKGLLYSIGLDSPMD
SRVIMIGQPEPAETPIQWVEDEYQLLKALIAWFEQFDPDVIIGWSVVDFDFRLLHKRAEF
HKLKLTIGRAQQPSFFRTASQTQQGFISIPGRVVLDGIDTLKTATYHFRSWSLESVSQEL
LGEGKAIHNVHDRMDEINQMFRHDKPSLARYNLQDCVLVNKIFAATHLLDFAIQRSRLTG
VELDRIGGSVAAFTNLYLPQLHRAGYVAPNLQPENWVASPGGYVMDSIPGLYDSVLVLDF
KSLYPSIIRSFLIDPLGLIEGLKLPIGKQADHAVPGFRGGQFHRTKHFLPEMIEKLWAAR
DEAKRNQEKAFSQAIKIIMNSFYGVLGSSGCRFFDARLASSITMRGHEIMIQTKRLIEAR
GYQVIYGDTDSTFVALGGQYSQQEADNIGHALVREINQWWTEHLKQEYALTSILELEYET
HYRRFLMPTIRGSETGSKKRYAGLKGDGDNEQLIFKGLESARTDWTPLAQRFQHQLYQLI
FHGQDPESYIRTIVEQTLAGQLDDQLVYQKRLRRRLHEYQKNVPPQVRAARMADDINAKL
GRPLQYQYRGTIEYVITVNGPEPKEYSKSPIDYQHYIDKQLKPVADAILPFIGKQFDELI
APQLGLFL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2364
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable