Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH200629.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
VcCD00020043
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3156nts
Open reading frame: 1 to 3156
ATGCGTGTACTCGAAGGAAAAGCAGGGAAGAGTGTTTTTACACTAAGCACTTTAACCGCT
TCATGTTTAATGGCTTTTAGCAGCTATGCTGCGGTGGATTGCTCGACGTTACCTGAATGG
CAATCGTCTGCGGTGTATACCGGTGGTGCCAAAGTGCAGCACAAGGGCAATGCTTACCAA
GCCAACTACTGGACTCAAAACAACGATCCTGAAACCTTTTCTGGTAACTATGCCCAATGG
AAACTGTTGGATTCATGCAGCACATCGGGGGGGACTAACCAAGCACCGAGTGTGACGTTA
ACCGCACCGCTTGCCACTGCGCAGGTGAAAGCCGGAGAGGTCATGACACTCGCAGCAAAC
GCCTCTGATGCAGATGGTTCTGTCGCGCGTGTTGAGTTCTCAGTGGATGGCGTATTGGTT
GGTCAGGCAACCAAAGCCCCGTACAGCGCGAGCTGGACAGCGACCAAAGGAACGCATGAA
TTTAGTGCGGTTGCCTTTGATGATAAAGGGGCTGTCAGCACCAAAAATGCAGTCACTCTG
ACGGTGAGTGACGCTGGTCCAGCGAATCAAGCGCCAACCGTGGCGGTGGCTCTCTCTGCA
TCAAGCGTGGATGTTGGGACGGTCGTAACGTTGAGCGCAGAAGCGGCGGATGCAGATGGC
TCAGTAGAGAAAGTCGATTTCTATGTTGGTGGTGCGCTTGTCGGCACTTCAGCAAAAGCA
CCTTACACTCTCAATTACACGGCAACCAAAGCAGGTTCACTAGCGGTTTACGCGCGTGCT
ACCGACAACTTAGGGGCAGCCACAGATTCTGCACTGACCACGCTGGTGGTGAATGGCGTC
GCACCGGTTGCGAATTGCCGTCCGGATGGTTTGTATCAAACGGAAGGCGTACAAGTGCCA
TACTGTACCGTTTACGATGCAGATGGCCGTGAGAAAATGGGCGTGGATCATCCACGCCGT
GTGATTGGTTACTTTACCAGCTGGTGTTCAGGCGATGATCCGCAAGCCGCTTACTTGGTT
AAAGACATTCCTTGGGAGCAACTGACACACATTAACTATGCGTTTGTCAGCATTGGTTCG
GATGGCAAAGTGAATGTCGGTGATGTCAACGATCCAAATAACGCGGCCGTTGGTAAAGAG
TGGCCAGGGGTAGAAATCGATCCAACGCTAGGTTTTAAAGGTCACTTTGGTGCTCTAGCC
ACTTACAAACAAAAATACGGTGTAAAAACCCTGATCTCTATCGGCGGATGGGCCGAAACC
GGCGGTCACTTTGATGCAAATGGTAACCGTGTGGCTGATGGTGGCTTCTACACTATGACC
ACCAATGCTGATGGTTCCATCAACCACGCAGCGATTGAAAAATTTGCGGCATCTGCAGTT
GAGATGATCCGTAAGTACAAATTTGATGGTGTGGATATCGATTACGAATACCCAACCTCC
ATGGCGGGCGCAGGAAATCCCGATGATAAAGGTTTTATGGAGCCACGTCGTGCTTACCTG
TGGGCTTCTTACCAAGAGTTGATGCGTGTTCTGCGTGAAAAACTCGATCAAGCCTCGGCG
CAAGATGGCCATCACTACATGCTGACGATTGCCGCACCATCTTCTGGTTACTTATTACGT
GGTATGGAAACATTCGATGTCACCAAGTATCTCGACTACGTCAATATCATGTCTTACGAC
TTGCATGGTGCGTGGAACGATCACGTTGGTCACAACGCCGCGCTCTACGATACAGGTAAA
GACTCTGAATTGGCGCAGTGGAACGTTTACGGTACAGCGCAATACGGTGGTATCGGTTAC
CTGAATACGGACTGGGCATATCACTACTTCCGTGGCTCAATGCCTGCAGGTCGTATCAAC
ATTGGTGTGCCTTACTATACCCGCGGTTGGCAAGGTGTCACCGGTGGTGAGAATGGACTC
TGGGGCCGAGCGGCACTGCCGAACCAAAATCTCTGTGCACCGGGTACTGGTGAAGGGGAG
AAAAACAACTGTGGTCACGGTGCAACCGGTATCGACAACATGTGGCATGACGTTAACGCT
GCAGGTGACGAAATGGGCGCAGGCTCTAACCCAATGTGGCATGCGAAGAACCTAGAAAAA
GGCATTTGGGGTTCATACGCAGCGGCTTATAAGCTCGATCCAACCACCACACCGCTGATT
GGTACTTATGTACGCAATTATGACAGTGTGGCCGTGGCTCCTTGGTTGTGGAACGCAGAG
AAGAAAGTGTTCTTGTCGACCGAAGATAAACAGTCAATTGATGTGAAAGCCGATTACGTT
ATCGACAAAGAGATTGGCGGTATCATGTTCTGGGAGTTGGCGGGTGACTATAGCTGTTAC
AACCTTGATGCCAACGGCAAGCGCACCTCAGTGGACCCTTCTGAGCAAGCGTGTGCCACC
GGTAAAGGTGAGTACCACATGGGCAATACCATGACCAAAGCCATCTACGACAAGTTCAAA
GTCGCCACGCCTTATGGTAACAAGGTGGCAACGGGCGCAATCCCTGCTGAAGCGGTGAAT
ATTGCGGTGAAAGTCGGTGGCTTCAAAGTCGGTGACCAAAACTACCCAATCAATCCGAAG
ATTACCTTCACCAACAATACAGGGATTGATATCCCGGGAGGAACGGAGTTCCAATTCGAT
ATTCCTGTTTCAGCGCCAGATAACGCGAAGGATCAATCGGGTGGCGGCTTGAAAGTGATT
GCATCAGGTCATACCCGTGCCAATAACATTGGCGGGTTAGACGGTACGATGCATCGCGTG
GCGTTCTCTCTACCCGCTTGGAAATCACTTCCTGCTGGGGCAACCTATGAATTGGATATG
GTTTACTACCTGCCGATTTCAGGTCCAGCGAACTACACAGTGAAAATCAACAATGTGGAG
TACGCGTTTAGCTTTGAGCAACCGGATCTCCCTGTCGCCGATTTGACTGCAGGTGGCAAT
ACGGGAGGTAATACCGGCGGTAACACTGGTGGTGGTACGGGTGGTGGCTCAACGGGTGAT
GTCATCCAGTGGGTTCCCGGTTCGACCCAAGTCCAAAATGGCACAACGGTGACTTACAAC
GGTAAATGCTTTGTAGCTCAAAATAACCCCGGCGTATGGGAATCACCGACACAATCTAAC
TGGTTCTGGAAAGAAGTGACTTGCCCTCAGGCGTTG
Protein sequence predicted based on nucleotide sequence
MRVLEGKAGKSVFTLSTLTASCLMAFSSYAAVDCSTLPEWQSSAVYTGGAKVQHKGNAYQ ANYWTQNNDPETFSGNYAQWKLLDSCSTSGGTNQAPSVTLTAPLATAQVKAGEVMTLAAN ASDADGSVARVEFSVDGVLVGQATKAPYSASWTATKGTHEFSAVAFDDKGAVSTKNAVTL TVSDAGPANQAPTVAVALSASSVDVGTVVTLSAEAADADGSVEKVDFYVGGALVGTSAKA PYTLNYTATKAGSLAVYARATDNLGAATDSALTTLVVNGVAPVANCRPDGLYQTEGVQVP YCTVYDADGREKMGVDHPRRVIGYFTSWCSGDDPQAAYLVKDIPWEQLTHINYAFVSIGS DGKVNVGDVNDPNNAAVGKEWPGVEIDPTLGFKGHFGALATYKQKYGVKTLISIGGWAET GGHFDANGNRVADGGFYTMTTNADGSINHAAIEKFAASAVEMIRKYKFDGVDIDYEYPTS MAGAGNPDDKGFMEPRRAYLWASYQELMRVLREKLDQASAQDGHHYMLTIAAPSSGYLLR GMETFDVTKYLDYVNIMSYDLHGAWNDHVGHNAALYDTGKDSELAQWNVYGTAQYGGIGY LNTDWAYHYFRGSMPAGRINIGVPYYTRGWQGVTGGENGLWGRAALPNQNLCAPGTGEGE KNNCGHGATGIDNMWHDVNAAGDEMGAGSNPMWHAKNLEKGIWGSYAAAYKLDPTTTPLI GTYVRNYDSVAVAPWLWNAEKKVFLSTEDKQSIDVKADYVIDKEIGGIMFWELAGDYSCY NLDANGKRTSVDPSEQACATGKGEYHMGNTMTKAIYDKFKVATPYGNKVATGAIPAEAVN IAVKVGGFKVGDQNYPINPKITFTNNTGIDIPGGTEFQFDIPVSAPDNAKDQSGGGLKVI ASGHTRANNIGGLDGTMHRVAFSLPAWKSLPAGATYELDMVYYLPISGPANYTVKINNVE YAFSFEQPDLPVADLTAGGNTGGNTGGNTGGGTGGGSTGDVIQWVPGSTQVQNGTTVTYN GKCFVAQNNPGVWESPTQSNWFWKEVTCPQAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3156
Mutations:
None
Discrepancy:
c985t,R329C
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
