Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH200659.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
VcCD00020069
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2550nts
Open reading frame: 1 to 2550

ATGAGATTAGAGAAGGGTATGCCTTTAAAACGCCAAATTCGCTTAAAAACCGCCATCATA
CTGCCGTTTGTCTTGACCTTTCTGTTCATGATTTTGGCAATGGCGGCGGTGCAAACCTAT
CGTTATGAGCAAACGGTAAAAGAGCTCAGCTCTAAGAAGTTATCTTATCTGACTGATAGC
ATCTCACAACGCTTGTCGGACTTTCTTAATCGCCCTTTTTTTGCCAATCAGATGATTGCC
TACAACGTGGGCTTTCACCATCTTTATCAGCTCAATGACGTAAGTCGAATTGAAGACTTC
ATTCGTGCGGCAGCCAATCCCATAGGTAACAATATCCAGCAAATCGACGTGGTCGGTTTT
GGTGGAGTGAATGGTGAATATGTAGGACTGCGTCGAGATGCACCAGAGCAATACAGCCTA
ATGCTCAAAGATGCACGCACTGACGATAAACTAGTTATTTATCAAACTGCGACAAAGAAT
GATCAATTGCGTACGGTGATTGATAACTACGATCCTCGCGTGCGGCCTTGGTTTGCCCCC
GTCGCGCAGAAGCCATCGCCACAATGGTCTTCGGTTTATACCAATATGGATGAGAAGCAA
GAGATCACTCTCTCCGCACTCAGCCCGGTTTTTCAGGATAAAAGATTTATTGGCGTCATG
GTTTCTGATGTCAAACTCAATACGTTCAACCAGTTTCTCTCCGAACTCAAACAACGTATG
AATGCTGACGTTTATGTGATGGACAAACAGCACCGCCTGATTGCCCATTCCGGTGAGGGT
AGCGTGGTTTCTTGGGGGACACCGTTAAGCCCGAAAGGTGAAAGGCTGTTCGCCAGCGAG
AATCGTAACCCCATCATTCGTAGCAGCGCCGAACAACTTGATCTGCAAGGCCTAAATGTG
GGTACTTTTACTACCTACGTCAATCAACAGCGCTATTTCAATCAAGCCTCAGCCTTTACC
GATCAATATGGCCTCACTTGGTATATCAGTGTTTCCATCGCGGAAGGGGATCTCCTAGGC
AGCTTACCTGAAAATCAAAAAACCAGTTGGGCGATAGGATTATTAGTGAGCTTAGTGGGT
TTAGGGCTCGGCTTAATGGCATTAAACCGCGTCGCCAACCCGATCATTTCTACCGCCTCG
GCAGCAAGACAACTTGCCAATGGTGACTGGACAGCCAGCATGCCTAAGCCCGGTAAAATC
TATGAGACCAGTTTGCTTGTCCAAGCCTTTATTGAGATGACCAATAGCCTCAAAGCCTCA
TTCAAAGCGCTACGCTCACAGTTGGTCTACGATTCTCTGACCCAACTTCTTAGCCGAGAG
GGGTTAGTTGAGCATTGTCATCAAATCCCGCAACTCAACGGGGGCCTTATCCTGATTGGG
ATTGATAAATTTCGCGATATTAACGACAGCTTAGGTCACCATCAGGCCGATCAACTGCTG
GTTGCTATTGCTCAAAGACTGAAAGTAACGTTTCCCGAGCCCGCTCTGATTGCTCGGATT
GGTGGCGATGAATTTGCTATCTACCTACCCGATGTGAATCAGCAAGATGATTTAAAAAGT
GTGGCTACCGATATTCTCAAGCTGTTTGCCTCTCCATTTAGCATGCCAAGCGACAATATT
GCTGTGAATGTGTCGATCGGAATTGTGTACCTTGAAGAACCGAATATGACCGTTTGGCTG
CGTAATGGTAGCATCGCCCTCAGTAACGCAAAGGCAGAACATACGCATATCAGTTTTTAC
TCACCAGAGATGGCGAATGCTTCACGCAAAAGAATGCTCATGGTCACGCAGATAAAACTG
GCGCTCGATAATCAAGAGTTCGTTCCCTTCTATCAGCCGATAGTCGATTTAAATAGTGGT
GAAGTATTAGGCGCAGAAGCGCTTGCTCGTTGGATTTCCCCTGAATTCGGCTTGATCCCA
CCAATGGATTTTATTCCGATTGCAGAAGAGAGCGGATTGATCAATGCGATTGGCGAGCAG
ATCTTACGTCAAGCCTGTGCTGATACCGTTCGTGGTATTGCAGAAAATAAATGGCCATCG
ACGTTCCATATGCATGTCAATCTATCGGTTAATCAACTCTCTCATCCCGATTTTATCGCT
CAGTTACACAGCGTATTAGACGAATCCGGCCTACAGCCTACCAACCTGACACTCGAAATC
ACCGAATCACGCATTGTCGATAATGACCCCGTTATCTTGCACAATATGCTTGAGTTGAAA
AAAGCAAGAATACACATTGCAATCGATGATTTTGGTACAGGCTATAGTTCTCTCGCCTAC
TTACACAAACTTCCCTTCGATTGCCTAAAAATTGATAGAGAATTTGTCTCTCAACTCAAT
GCATACAATATTGAAAACAGCGTGGTAGCCGCGATCGTTAACATGACTCGCGGTTTTAAA
GTCGAGCTGGTCGCTGAAGGCATAGAGACTCAAGAGCAGGTGGATTTACTGAATCAACTC
GGTTGCCCACGCGGGCAAGGCTTCTTATTCAGTAAACCAATGGCTTATCAGGATTGGCCA
ACAGATCTGGTTAACATGAAGCAAAGTTTG

Protein sequence predicted based on nucleotide sequence

MRLEKGMPLKRQIRLKTAIILPFVLTFLFMILAMAAVQTYRYEQTVKELSSKKLSYLTDS
ISQRLSDFLNRPFFANQMIAYNVGFHHLYQLNDVSRIEDFIRAAANPIGNNIQQIDVVGF
GGVNGEYVGLRRDAPEQYSLMLKDARTDDKLVIYQTATKNDQLRTVIDNYDPRVRPWFAP
VAQKPSPQWSSVYTNMDEKQEITLSALSPVFQDKRFIGVMVSDVKLNTFNQFLSELKQRM
NADVYVMDKQHRLIAHSGEGSVVSWGTPLSPKGERLFASENRNPIIRSSAEQLDLQGLNV
GTFTTYVNQQRYFNQASAFTDQYGLTWYISVSIAEGDLLGSLPENQKTSWAIGLLVSLVG
LGLGLMALNRVANPIISTASAARQLANGDWTASMPKPGKIYETSLLVQAFIEMTNSLKAS
FKALRSQLVYDSLTQLLSREGLVEHCHQIPQLNGGLILIGIDKFRDINDSLGHHQADQLL
VAIAQRLKVTFPEPALIARIGGDEFAIYLPDVNQQDDLKSVATDILKLFASPFSMPSDNI
AVNVSIGIVYLEEPNMTVWLRNGSIALSNAKAEHTHISFYSPEMANASRKRMLMVTQIKL
ALDNQEFVPFYQPIVDLNSGEVLGAEALARWISPEFGLIPPMDFIPIAEESGLINAIGEQ
ILRQACADTVRGIAENKWPSTFHMHVNLSVNQLSHPDFIAQLHSVLDESGLQPTNLTLEI
TESRIVDNDPVILHNMLELKKARIHIAIDDFGTGYSSLAYLHKLPFDCLKIDREFVSQLN
AYNIENSVVAAIVNMTRGFKVELVAEGIETQEQVDLLNQLGCPRGQGFLFSKPMAYQDWP
TDLVNMKQSL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2550
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable