Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH200920.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
VcCD00020286
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2940nts
Open reading frame: 1 to 2940

ATGATGAGTGATAGACAAAGTTTTCTGTATTCAGGTCATAAGCTCTCTTCTGGTGGTGCA
AACGATCCGCTCCTACCAAGACTTGTCCAAGCCATTAATCACGCGACTGAAATTGAAATT
TCAGTCTCATTCATTCAGCCATCCGGTCTGGATTTACTGTTTGATCCTCTGTTTGATGCT
GTGCAAAGTGGTGCTCAGGTAAAACTCCTAACATCAGATTATCTCTCGATTACTCATCCT
GTTGCGCTGCGACGCCTAATGTTGCTAACAGAACGCGGCGCTCAGTGTCGTGTATTTGAA
TGTGGTCAGCACAGCTTTCATATGAAATCGTACATTTTTGTCCGATGTGAACAGGGAGAA
ATACTTGAAGGTTGCGCTTGGATTGGTTCCAACAACATCAGCAAAACAGCGTTATTAGAC
AGCCATGAATGGGCTCTACGTCACGACTTTGAACCACCTGAAACCAGTGCTGCTGCCCTT
GAGTTTCTCCATATTCGTCAACAGTTTGCTTCCATTTTCAACCACACAAATAGTAAAGAT
TTAACGCATACTTGGATTGATCATTACCTTGAACGCTATCAACAAGCGAAAAAGCAGCAC
GGCATGCCCATACTGGCCGATAGCCAAGATGAACAGTCAGAACCGCCAGCGCCGAATGCA
GTTCAAGTAGAGGCGTTAACCGCATTGAACGCCACTAGAGCCCAAGGATTTTCTCGTGGA
TTAGTCGTGCTAGCTACGGGCATGGGCAAAACGTGGTTAGCAGCATTTGATGCTCTACAA
ACTCAGTCGACAACAGTCTTGTTCGTTGCGCATAGAGAAGAAATTCTTCTGCAAGCGGAA
AAAACCTTCTGTCAGCTTATTCCCAATGCAAAAACGGGTCTCTACAACGGTGTAACGCAA
AATACACAAGCGATGCTGCTGTTTGCTTCCGTGGCAACCATAGGTAAACAAAATCACCTG
CAACGCTTTGCAGCGGATCACTTTGATTACATTGTGGTGGATGAATTTCATCACGCGGCA
GCGAGAAGTTACCGAAACTTACTCACCTATTTTAAGCCTAAATTTCTCCTCGGCCTAACC
GCAACACCGGAACGTTCAGATCAAGCGGATATTCTCTCGCTTTGCGACAGTAATTTAGTC
TTTGAACGTAACCTTGTTCATGGAATTGATGAGAAAATTCTGGTTCCTTTTGACTATCAC
GGTATCTACGACCAAGCCGTGAACTACCAAGAAATCCCATGGCGTAACGGTAAATTCGAC
CCAGACTCACTCGATAATGCCCTAGCAACTCAACGCCGTGCCGAACACGTTTACCAGCAT
TGGCACCAGAAAAAACAAACCCGCACACTGGCTTTTTGTGTTTCTAAAAAACACGCAGAC
TTCATGGCTGAGTTTTGTCTGAGTAAAGGCATAAAAGCGATTGCGGTTTACAGTGATTCA
AAGGTTCGACGTAACCAAGCCTTACAGTGGCTAGATTCAGGAAAAATAGACATCCTTTTC
TCCGTCGATCTTTTTAATGAAGGTACCGATCTGCCCGCTATTGATACCATTTTGATGCTT
CGTCCAACGGAGTCTAAAATTCTCTTTTTACAGCAGTTGGGACGGGGATTGCGTCGAAGC
ATTGAAACTCAAAAAAGTAAACTGGTTGTTATTGATTTTATTGGTAATCATGACTCGTTC
TTAAATCGTCCAACCACACTCTACAATGTGAGTCATTTAAAAGACGCCTTAGCCAAACAC
CAACAACAAGCATTACCTGACGGATGCCATGTCACTTTTGATATCACTTTGCTCAACTTT
TGGCAGCAATTAACCCGGAAAATGCGTTTCTCAGTGCAAGATGAGTATCAGCAACTTGCA
CACCAGCTCGCACATAGACCAACTGCTAGTGAGTTTTTCTATCATGGAATTGAAATAAGT
AAAGTGCGTAAACAAGCGCAAAGCTGGTTTCATTTGGTTGCGAGCCAAGAAAATGATCCC
GAGTTGGCTGAGATTGTTACACGCTATGGTGATTTTCTTCTGCATGGCATTGAAAGCACC
GGCATGAGTAAATCTTTCAAAGCAATTCTACTTGAGGCCTTACTCGAACTGGATGGATTA
AGAACTCCGCCGACCTTAGCCACATTGGCTGAATGCAGTTACACTGTTATCGCACGCAGA
CCAGACCTCATGGCTGAAGACCTAACTGAAAATGCCAAACAATTTAAAGCAGCAGATAAA
GATTGGCTTAACTATTGGCGGAACAACCCTATTAAGGCCTTTACGAACAAAGCAACGAAA
CAGGCCACTTGGTTTTCCATCGATAGCCAACAACGCTTCGTCGCTAACTTTGATATCCGA
GAGCAAGATCTAGAACGGTTACACGATTGTATACAAGAATTGGTTGATCTGCGTTTGGCG
GAATATGCTCAGCGACCCAAGCAACATAAAGAAAATCTTATTAAGGCTAAGAGCAAAGCT
GCTGTCATTTCATTAATACCTGAACAAAAAACGATAGGAACACCTATCCCATTCTATCCA
GAGCTCAAAATTGCCTGTGGCCATTTTAAGCGCGGTTCAGAGCGAGAAGTACAGACTTAT
TTTGTTCCTGATGGCTATGGATTACTTGATCCCACACGGCATTTTGTCTCGCCAGCCTCT
GGTAACTCAATGAATGGCGGTAAAAATCCTATTCAAGATGGTGACTTATTATTGCTTGAG
TGGGTGACACCAAGCAGTGCTGGCTCTATATCAAACCTCACCATGGCTATAGAAACTCAA
GATGAAACGGGGGATAACCAGTACTTACTGCGGGTAGTACGTAAAATCGCACCAAACCAG
TATGAACTACAAGCACAAAATCCAAGTTATCCAAATATGCCAGCAACCGATGCAATGAAA
ACGTTCGCACGGTTAAAAGCTGTGATAAAAAATAATAGTCAGCAATCACAGACTCGATTG

Protein sequence predicted based on nucleotide sequence

MMSDRQSFLYSGHKLSSGGANDPLLPRLVQAINHATEIEISVSFIQPSGLDLLFDPLFDA
VQSGAQVKLLTSDYLSITHPVALRRLMLLTERGAQCRVFECGQHSFHMKSYIFVRCEQGE
ILEGCAWIGSNNISKTALLDSHEWALRHDFEPPETSAAALEFLHIRQQFASIFNHTNSKD
LTHTWIDHYLERYQQAKKQHGMPILADSQDEQSEPPAPNAVQVEALTALNATRAQGFSRG
LVVLATGMGKTWLAAFDALQTQSTTVLFVAHREEILLQAEKTFCQLIPNAKTGLYNGVTQ
NTQAMLLFASVATIGKQNHLQRFAADHFDYIVVDEFHHAAARSYRNLLTYFKPKFLLGLT
ATPERSDQADILSLCDSNLVFERNLVHGIDEKILVPFDYHGIYDQAVNYQEIPWRNGKFD
PDSLDNALATQRRAEHVYQHWHQKKQTRTLAFCVSKKHADFMAEFCLSKGIKAIAVYSDS
KVRRNQALQWLDSGKIDILFSVDLFNEGTDLPAIDTILMLRPTESKILFLQQLGRGLRRS
IETQKSKLVVIDFIGNHDSFLNRPTTLYNVSHLKDALAKHQQQALPDGCHVTFDITLLNF
WQQLTRKMRFSVQDEYQQLAHQLAHRPTASEFFYHGIEISKVRKQAQSWFHLVASQENDP
ELAEIVTRYGDFLLHGIESTGMSKSFKAILLEALLELDGLRTPPTLATLAECSYTVIARR
PDLMAEDLTENAKQFKAADKDWLNYWRNNPIKAFTNKATKQATWFSIDSQQRFVANFDIR
EQDLERLHDCIQELVDLRLAEYAQRPKQHKENLIKAKSKAAVISLIPEQKTIGTPIPFYP
ELKIACGHFKRGSEREVQTYFVPDGYGLLDPTRHFVSPASGNSMNGGKNPIQDGDLLLLE
WVTPSSAGSISNLTMAIETQDETGDNQYLLRVVRKIAPNQYELQAQNPSYPNMPATDAMK
TFARLKAVIKNNSQQSQTRL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2940
Mutations:
None
Discrepancy:
a794c,K265T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable