muts (F. tularensis subsp. tularensis SCHU S4 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH153676.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
FtCD00020672
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2574nts
Open reading frame: 1 to 2574

ATGCTAACAAAAAGATTCTACAATTTAGCTATTATGCAAGATATTTCTAATCACACCCCG
ATGATACAACAATATTTGAAAATTAAATCACAGTATCAAGATATATTATTATTTTACCGT
ATGGGCGATTTTTATGAGCTTTTTTTTGATGATGCTAAAAAAGCTGCTGAGCTCTTAGAT
ATTACGCTCACAGCTCGTGGTAAATCAAATGGTGAATCAATCCCGATGGCTGGAGTGCCA
TATCATGCCGCTGAAGCCTATATTGCAAAAATTGTCAAAAAAGGTCTATCGATTGCTATC
TGTGAGCAAACTGGCGATCCAAACACCTCAAAAGGTCCCGTTGAGAGACAAGTGACACGT
ATTATAACTCCAGCGACAGTTTCTGAAGAAGCTTTTTTAGACAACAATCAAGATAGTATT
CTTGTGAGTATTTTTGAAAAAAATAATAAGTATTATTTAGCATACACAAGCTATACGCAA
GGAAAAATTTATCTAGTTAAAACACTAACTAGCCTAAATGAGCTAAAAAATACTGTTCTG
AAATTATCACCTCAAGAAATTATTACTAACTCTCGCGAACTTGCTCAGCAGAATCCTTTT
AAAAAACCAATTAAAGCTTTAGAAGAATGGTATTATAGTAATTTTGAGGCAAAAAAATAT
ATCAATGATTCTCTTGATACCAATATTGCTAATAATATCCTAAATCTTTATAAAAATGAT
CAACTAACAACTATTGGCTCAATACTTAGCTACCTTACAAATATTCTCAAGGATACTCCT
AGGCATATTACTGATATCAGCTATGAACAAGAACAAGATACGCTTAACATTGATATAAAT
AGTCGTATAAATCTTGAGCTAGATAATAACTCAAAAAGTAGCTTACTTAGCATAATTGGT
AAATGTAAGACTAGCCTTGGTAGTCGTTTACTAAAAAGGTATTTTAGCAATCCAACAAGA
AATTTAAACATTTTAGCTACTCGCCATAGTATTATAAACAGTTTAGGTGAAAATCAACAT
TTTTTAAAAATTCAAGATGTGCTTAGTTATATCAGTGATATTGAAAGGATAATCTCACGA
GTAGCACTTGGAACTGTAAAACCTAAAGATCTTGTCGCTCTGCGTGACTCCCTTGAGCAG
CTACCAATACTCAAAAAACTCCTCAGTGAAAAAAATACTCCAGAGATTACAAATATAAAT
AACCGCATCCACCAGCTTGATGAGCTTGTTACACTTTTAGATAAAGCGATTATTGAGAAT
CCCCCAACAACCATTCGTGATGGTGGCGTCATCAAAGAAGGTTTTGATAAAGAACTAGAT
GAGCTAAAAAGCATAAAAGATAATTCTTATGATTTTCTAATCAAGTTTGAAGAATTACAA
AAACAGAAAACTGGTATAAGTACACTCAAAGTTGGTTACAATAGTGTTCATGGCTATTAT
ATTGAGTTATCTAAACAGCATGCTGATAAAATCCCAACTGAGTATGTCAGACGCCAAACC
TTAAAAGCTAGTGAACGCTATATCACCGAAGAGCTAAAAAACTTCGAAGATAAAGTCCTT
TCATCAAAAGAAAAGGCTCTAGCCCGTGAAAAGTTAATCTATGATACTCTATTAAAGAAA
GTTATCGAATACTACAAACAAATTCAAGAAACTGCTGCAAGTATTGCTGAAATAGATGTT
TTAGCAAATTTTGCTGAGAGAGCTATTAAGCTTAAACTTAGTCAACCTAAGTTTAATAAC
TTAGCAAAATTAGAACTTAAAGAAGTTCGTCACCTTGCTATTGAGCATAATATTGATGAA
CCATTTATCCCAAACGATACTTTACTAAGCAAAGATACTAATACCCTACAAATAATAACT
GGTCCAAATATGGGCGGTAAATCGACATATATGCGCCAAGTTGCACAGCTAATATTTCTA
GCGTATATCGGCTCATTTGTACCAGCAAGTTATGCAGATATTTGTGATATTGATACTATC
TACACAAGGATTGGTGCATCTGATGATATTTCTAGCGGTAGATCAACTTTTATGGTTGAA
ATGACTGAAACAGCCTATATTCTCAATAATGCTAGTGCCAAATCACTAGTAATAATGGAT
GAAATAGGTCGTGGCACAAGTACTTTTGATGGCTTAGCATTAGCTAAAGCATGTGCAGAG
AAGTTTGCACAAATAGGGGCTTTTACTTTATTTGCAACACATTATTTTGAGCTAACAGAA
TTGGCTAAACAATATCCAAATGTATGTAACATCCATTTTGAAGCAAAAGAATATAAAGAT
AATATCTACTTTATGCACAAAGCTGTTACAGGAGCGGCTAAAAAATCCTATGGCATCCAA
GTAGCCAAACTTGCCGGAATATCTCAAGATGTCCTAGAGTCAGCAAAGCAAAATTTATAT
AATCTCGAAAAAAAGCAACAGCTAACAGAATCAACCCAAGTCCAAGCTCAATTTCAGTTA
GAACCAACAACTCAAAATCCTTTACAACAAAAACTTGATGCTATCGATATTAATACAATA
ACACCTCTAGAAGCACTAAATATTCTCTTTGAACTAAAAAAAACGCTAATTTAG

Protein sequence predicted based on nucleotide sequence

MLTKRFYNLAIMQDISNHTPMIQQYLKIKSQYQDILLFYRMGDFYELFFDDAKKAAELLD
ITLTARGKSNGESIPMAGVPYHAAEAYIAKIVKKGLSIAICEQTGDPNTSKGPVERQVTR
IITPATVSEEAFLDNNQDSILVSIFEKNNKYYLAYTSYTQGKIYLVKTLTSLNELKNTVL
KLSPQEIITNSRELAQQNPFKKPIKALEEWYYSNFEAKKYINDSLDTNIANNILNLYKND
QLTTIGSILSYLTNILKDTPRHITDISYEQEQDTLNIDINSRINLELDNNSKSSLLSIIG
KCKTSLGSRLLKRYFSNPTRNLNILATRHSIINSLGENQHFLKIQDVLSYISDIERIISR
VALGTVKPKDLVALRDSLEQLPILKKLLSEKNTPEITNINNRIHQLDELVTLLDKAIIEN
PPTTIRDGGVIKEGFDKELDELKSIKDNSYDFLIKFEELQKQKTGISTLKVGYNSVHGYY
IELSKQHADKIPTEYVRRQTLKASERYITEELKNFEDKVLSSKEKALAREKLIYDTLLKK
VIEYYKQIQETAASIAEIDVLANFAERAIKLKLSQPKFNNLAKLELKEVRHLAIEHNIDE
PFIPNDTLLSKDTNTLQIITGPNMGGKSTYMRQVAQLIFLAYIGSFVPASYADICDIDTI
YTRIGASDDISSGRSTFMVEMTETAYILNNASAKSLVIMDEIGRGTSTFDGLALAKACAE
KFAQIGAFTLFATHYFELTELAKQYPNVCNIHFEAKEYKDNIYFMHKAVTGAAKKSYGIQ
VAKLAGISQDVLESAKQNLYNLEKKQQLTESTQVQAQFQLEPTTQNPLQQKLDAIDINTI
TPLEALNILFELKKTLI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2574
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable