Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH154053.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
FtCD00020713
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 420nts
Open reading frame: 95 to 420

TTGAAAAAGTTAATTACACTATCAACATTACTATTAATATCTCTAACTAGTATTGCTTTT
AGTAAAGAGTTAAATAATTATTCTGATATTCTAAATGCTGTAAAAGATGGAAAAAATATT
ACTATCTTTGTTGATTTCTTAAATTGTAAACCAGAGATAAAAGTATCTGGGCAATTTAGT
CCTAAATCAATAATGATTCACAATGATTCTATAATTTTTTCAGATACCCATTTTACTAGA
AATAATCCACAATACCCAAATGAGCCAATACTAGAATATGTGGTTTATAAAATAAATGGT
AATAATGTTAATATAACAATAGATATTCTTGATGCTAATAATAGTCCCATGGAACACTCT
AAACGTATTACAATTGGCTGCCAAATTACTAAAGATCAAGCTAGCTTCTTCTCTAACTAG

Protein sequence predicted based on nucleotide sequence

ML*KMEKILLSLLIS*IVNQR*KYLGNLVLNQ**FTMIL*FFQIPILLEIIHNTQMSQY*
NMWFIK*MVIMLI*Q*IFLMLIIVPWNTLNVLQLAAKLLKIKLASSLT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 420
Mutations:
None
Discrepancy:
a1t,M1L; a10t,I4L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable