Gene:
Gene Symbol:
MAP2K1
Gene Name: 
mitogen-activated protein kinase kinase 1
Original Clone ID:
FLH163704.01F
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format:
FUSION
Source:
HIP
Description:
None
Comments:
both tube and plate source failed to sequence with M13F primer, poor sequence reads; please remove
Mutation / Discrepancy:
No / No
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00021317
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT
AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA
GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG
GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT
GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT
CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG
TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT
ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA
ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG
AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC
CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC
AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC
TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG
TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA
GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC
GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG
CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA
TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT
ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC
CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTTG
Protein sequence predicted based on nucleotide sequence
MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKV GELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHE CNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYL REKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHY SVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSY GMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAF IKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
1254
Start on reference sequence
73
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDNR-Dual
Synonyms:
''
Sequencing Primer:
Forward:
M13F
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Creator, donor (entry), loxP, multiple cloning site, recombinational cloning clone with LoxP and 6X His and T7 Terminator and CmR and sopB and sacB and AmpR promoter and AmpR and ori and M13 forward and T7 promoter; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp):
4938
Parent Vector:
None
Properties:
Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
6X His
6x(His-Asn) affinity tag
141
176
CDS
CmR
confers resistance to chloramphenicol
327
986
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
3103
3963
CDS
sacB
secreted levansucrase that renders bacterial growth sensitive to sucrose
1509
2930
Terminator
T7 Terminator
transcription terminator from phage lambda
212
306
primer site
T7 promoter
promoter for bacteriophage T7 RNA polymerase
4863
4881
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
4839
4855
promoter
sopB
sacB promoter and control region
1063
1508
promoter
AmpR promoter
null
2998
3102
protein binding site
LoxP
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
9
42
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4134
4722
Gene
Gene Symbol :
MAP2K1
Symbol Nomenclature :
MAP2K1
SYNONYM :
CFC3; MAPKK1; MEK1; MKK1; PRKMK1
Designation :
ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|dual specificity mitogen-activated protein kinase kinase 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Full Nomenclature :
mitogen-activated protein kinase kinase 1
GENEID :
HGNC :
MIM :
Target GenBank:
Reference Sequence Alignment
HsCD00021317 ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT NM_002755.3 ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT ************************************************************ HsCD00021317 AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA NM_002755.3 AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA ************************************************************ HsCD00021317 GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG NM_002755.3 GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG ************************************************************ HsCD00021317 GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT NM_002755.3 GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT ************************************************************ HsCD00021317 GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT NM_002755.3 GTGGTGTTCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT ************************************************************ HsCD00021317 CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG NM_002755.3 CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG ************************************************************ HsCD00021317 TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT NM_002755.3 TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT ************************************************************ HsCD00021317 ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA NM_002755.3 ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA ************************************************************ HsCD00021317 ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG NM_002755.3 ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG ************************************************************ HsCD00021317 AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC NM_002755.3 AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC ************************************************************ HsCD00021317 CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC NM_002755.3 CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC ************************************************************ HsCD00021317 AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC NM_002755.3 AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC ************************************************************ HsCD00021317 TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG NM_002755.3 TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG ************************************************************ HsCD00021317 TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA NM_002755.3 TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA ************************************************************ HsCD00021317 GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC NM_002755.3 GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC ************************************************************ HsCD00021317 GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG NM_002755.3 GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG ************************************************************ HsCD00021317 CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA NM_002755.3 CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA ************************************************************ HsCD00021317 TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT NM_002755.3 TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT ************************************************************ HsCD00021317 ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC NM_002755.3 ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC ************************************************************ HsCD00021317 CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTTG NM_002755.3 CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAA ****************************************:.
Pathways
NCI :
BCR signaling pathway
NCI :
Cellular roles of Anthrax toxin
NCI :
Ceramide signaling pathway
NCI :
Class IB PI3K non-lipid kinase events
NCI :
CXCR3-mediated signaling events
NCI :
Downstream signaling in naïve CD8+ T cells
NCI :
Endothelins
NCI :
EPHB forward signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
FOXM1 transcription factor network
NCI :
GMCSF-mediated signaling events
NCI :
Hedgehog signaling events mediated by Gli proteins
NCI :
IFN-gamma pathway
NCI :
IL2-mediated signaling events
NCI :
mTOR signaling pathway
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Nongenotropic Androgen signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Ras signaling in the CD4+ TCR pathway
NCI :
SHP2 signaling
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Trk receptor signaling mediated by the MAPK pathway
Panther :
Angiogenesis
Panther :
Angiotensin II-stimulated signaling through G proteins and beta-arrestin
Panther :
B cell activation
Panther :
EGF receptor signaling pathway
Panther :
Endothelin signaling pathway
Panther :
FGF signaling pathway
Panther :
Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade
Panther :
Integrin signalling pathway
Panther :
PDGF signaling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
Toll receptor signaling pathway
Panther :
VEGF signaling pathway
Reactome :
Activated TLR4 signalling
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling of activated FGFR
Reactome :
ERK activation
Reactome :
ERK1 activation
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin-1 signaling
Reactome :
Interleukin-2 signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
L1CAM interactions
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MEK activation
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
NCAM signaling for neurite out-growth
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Prolonged ERK activation events
Reactome :
RAF phosphorylates MEK
Reactome :
RAF/MAP kinase cascade
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
EPO Receptor Signaling
WikiPathway :
ErbB signaling pathway
WikiPathway :
Estrogen signaling pathway
WikiPathway :
Focal Adhesion
WikiPathway :
Hypothetical Network for Drug Addiction
WikiPathway :
IL-1 signaling pathway
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
IL-5 signaling pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
IL-7 signaling pathway
WikiPathway :
IL-9 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK Cascade
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
Nifedipine Activity
WikiPathway :
Osteopontin Signaling
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
Senescence and Autophagy
WikiPathway :
Serotonin HTR1 Group and FOS Pathway
WikiPathway :
Serotonin Receptor 2 and ELK-SRF/GATA4 signaling
WikiPathway :
Serotonin Receptor 4/6/7 and NR3C Signaling
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TSH signaling pathway
Protein
SMART domain :
SMART domain :
UniProt :
UniProt :
HPRD :
01469
Clone
Organism
Species Specific ID:
Genome
Chromosome :
15
Map Location :
15q22.1-q22.33
Reagents
Labome :