Gene:
Gene Symbol:
PIK3R4
Gene Name:
phosphoinositide-3-kinase, regulatory subunit 4
Original Clone ID:
FLH173819.01F
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00021462
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCTTTCTGTAGAGAGTTATTTT
TCAGATATTCATGACTTTGAATATGATAAAAGCCTGGGGAGTACTCGGTTTTTTAAAGTT
GCTCGAGCCAAGCACCGAGAAGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCC
ACATTGCCTTTAACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCT
GCACAGAATTTTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAAGCAGCTATG
CTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCATCAGTACCCGTCCATTCTTG
AATAACATTGAGAAGCGCTGGATTGCTTTCCAGATCCTGACAGCTGTGGACCAAGCACAC
AAATCTGGAGTTCGTCATGGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAAT
TGGGTTCTTCTAACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCG
GCAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATTGCTCCTGAA
CGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATATATGAGAGATCCTTCAACT
CCGCTTGTAGACTTAAATAGCAATCAGAGAACAAGAGGAGAGTTGAAGAGAGCAATGGAC
ATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGAT
CTCTCTCAACTTTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAA
ATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAGCCAGATAAA
CGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAATGCCTTTCCTGAAATATTT
TACACTTTTCTTCAGCCCTACATGGCCCAGTTTGCCAAGGAAACGTTTCTTTCTGCAGAT
GAGCGTATTCTGGTTATACGGAAGGATTTGGGCAACATTATTCACAATCTCTGTGGACAT
GATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTATCT
GTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGCTGCTTTGGAA
CTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCTTTTGGATCGTATTACTCCA
TATCTTTTGCATTTCAGCAATGACTCTGTTCCTAGGGTGAGGGCTGAAGCCTTGAGGACG
TTGACCAAAGTTCTTGCTCTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCG
GAATACATTCTGCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTA
GCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCTGGAATTAGTA
CAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATGAAGAAATAGATGAGGTTACA
CATCCAAATGGAAATTATGACACAGAGCTCCAAGCCTTACATGAAATGGTCCAGCAGAAA
GTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGA
ATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCAC
ATGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTTTTTGATAGT
ATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTCAATTCTCAAGCCTCTGCTG
CAACAAGGTCTTAGTGATGCTGAGGAATTTGTCATTGTGAAAGCTCTTTATGCCCTTACT
TGTATGTGCCAGTTAGGACTGCTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATT
GCCCCCTTCCTGTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCACA
GTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATGCCTTATCTTGAC
CCATATATTACCCAACCAATAATACAGATTGAAAGAAAACTTGTTCTGCTCAGTGTTTTA
AAGGAACCAGTAAGTCGTTCTATATTTGATTATGCTTTGAGGTCTAAAGATATTACTAGC
TTGTTCAGACATCTTCACATGCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCT
CCGCCAGAGGATCCTGCCATAGCACAGCTTCTGAAGAAGTTGCTCTCACAGGGAATGACA
GAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCATGATGAAATCTAATAAAGCA
AAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAGTAGTCAGAAAGGTGTAATTGAC
TTGGCAGCTTTAGGCATAACTGGGAGACAAGTTGATCTTGTTAAAACCAAACAAGAACCA
GATGACAAACGGGCCAGAAAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAA
AGCATGTTTGGGTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTGAT
CAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCCGAGTCCTCTGCTGGCATTTGTGTC
CCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACAACTGTCCAAAATAAAAAACCAGTA
ATACCGGTTTTAAGTAGTACAATCTTACCATCCACCTATCAGATTCGAATTACAACTTGT
AAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGGGAGCAGTGCAATGCTGAGAGAATA
GCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGCGT
CCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTGAATCGAATTAGA
GTCTCTGATGAACACTCACTTTTTGCAACATGTTCAAATGATGGCACAGTGAAAATCTGG
AACAGTCAAAAGATGGAGGGGAAGACCACCACTACCAGATCTATTCTTACATACAGCCGA
ATTGGAGGACGAGTCAAGACGCTCACATTCTGCCAAGGCTCCCACTATTTAGCCATAGCA
TCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTTCTAAGCTGCCCAAGTCTCCT
AAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGTTGTGTTGTGGAT
ATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGCCTATGCCACTGTGAATGGCTCT
CTGGTTGGCTGGGACCTTAGGTCTTCAAGCAATGCGTGGACTTTAAAGCATGATTTAAAG
TCGGGCCTCATCACTTCCTTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACA
AGCAGTGGTACCATGGCTTGTTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACTGT
CATCTTTCCAGGGCTCGAATCAGACGCCTCTCAATGCACCCTCTGTATCAGTCCTGGGTG
ATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTGGGACATGGAGACTGGTGACAGA
AGATTTACTCTCTGGGCCAGCAGTGCACCACCACTTTCTGAATTACAGCCTTCTCCTCAT
AGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGC
TCAGATATGAAAATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTGCA
GGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAAGGCACTGAAGTT
GTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCAAGTGATGACACCCCTCGAAGGGGC
CCAGAGTCCCTGCCCGTGGGACATCATGACATCATCACTGATGTCGCCACATTCCAGACC
ACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGGATTGTGAAGGTGTGGAAATTG
Protein sequence predicted based on nucleotide sequence
MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDP TLPLTSYKQELEELKIRLNSAQNFLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFL NNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNP ADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMD IFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDK RLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLCGH DLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITP YLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRL AYAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQK VVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDS IVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASDI APFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVL KEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQLLKKLLSQGMT EEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEP DDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICV PLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERI AKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIW NSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSP KIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLK SGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHLSRARIRRLSMHPLYQSWV IAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAG SDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRG PESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4619
Start on reference sequence
543
Mutations:
None
Discrepancy:
g251t,C84F; c3545t,P1182L
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDNR-Dual
Synonyms:
''
Sequencing Primer:
Forward:
M13F
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Creator™, donor (entry), loxP, multiple cloning site, recombinational cloning clone with LoxP and 6X His and T7 Terminator and CmR and sopB and sacB and AmpR promoter and AmpR and ori and M13 forward and T7 promoter; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp):
4938
Parent Vector:
None
Properties:
Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
6X His
6x(His-Asn) affinity tag
141
176
CDS
CmR
confers resistance to chloramphenicol
327
986
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
3103
3963
CDS
sacB
secreted levansucrase that renders bacterial growth sensitive to sucrose
1509
2930
Terminator
T7 Terminator
transcription terminator from phage lambda
212
306
primer site
T7 promoter
promoter for bacteriophage T7 RNA polymerase
4863
4881
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
4839
4855
promoter
sopB
sacB promoter and control region
1063
1508
promoter
AmpR promoter
null
2998
3102
protein binding site
LoxP
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
9
42
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4134
4722
