MAP4K3 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MAP4K3
Gene Name:  mitogen-activated protein kinase kinase kinase kinase 3
Original Clone ID:
FLH203240.01F
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00021481
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2685nts
Open reading frame: 1 to 2685

ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAG
CGCATCGGCAGCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAA
TTAGCAGCAATTAAAGTAATAAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAA
GAAATTATCATGATGAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTAT
CTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTACAGGAT
ATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAGCAGAGAAACA
CTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAG
ATAACAGCTACAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCA
GAAGTTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTG
GGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTTTGACTTACACCCAATG
AGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTCCTAAACTAAAGGATAAAATG
AAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAA
AGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTC
GATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGA
AACGTGAGAGAAGAAAAAACACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCA
CCCTTAAGAAAGGAGACAGAACCACATCATGAACTTCCCGACAGTGATGGTTTTTTGGAC
AGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGGATCTGCAACTGGAATATGGA
CAAGGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCTGTTGAA
GAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGATGAT
GAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAG
TCTATCTTCATACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAG
AGATGTCCCATGTCAGGGAGCCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCT
CCCAGATTACCCCCACACAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTA
AATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATGAACATAGAGGCACAAACCTT
TCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCTTCCTCCAACACCT
AAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCAC
TGTGCATCATCATGGATAAACCCAGATACTAGAGATCAGTACTTGATATTTGGTGCCGAA
GAAGGGATTTATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTC
CCTCGAAGGTGTACATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAA
GCTTCTCAGCTTTATTCCCATAATTTACCAGGGCTTTTTGATTATGCAAGACAAATGCAA
AAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTGACAGAATACTGCCAAGGAAATTT
TCTGTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGTTGTAAGAAAT
CCTTACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAA
TGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGT
CCACTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTT
GGTGTCAGTAGAGGTAGAGACTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAAT
TCTACCTCTTCATGGTTTACAGAATCAGATACCCCACAGACAAATGTTACTCATGTAACC
CAACTGGAGAGAGATACCATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTAAATCTC
CAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGATTTCCAGATT
GAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAATGCAAGGT
AGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGG
CTGCTTGGATCTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCA
AATAGCAATTTGTACATCCTGGCGGGTCATGAAAACAGTTACTTG

Protein sequence predicted based on nucleotide sequence

MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQ
EIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRET
LQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAP
EVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKM
KWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF
DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLD
SSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDD
ESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPP
PRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTP
KVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLF
PRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKF
SVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPC
PLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVT
QLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQG
RSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSYL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2776
Start on reference sequence 92
Mutations:
None
Discrepancy:
t189c; a1710t
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable