DGKH (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DGKH
Gene Name:  diacylglycerol kinase, eta
Original Clone ID:
FLH203845.01F
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00021501
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3303nts
Open reading frame: 1 to 3303

ATGCTGTGTGCTGAGAACAGAAAGGAGATGGAGGATTGGATCAGCTCACTGAAGTCTGTA
CAGACCAGAGAACCCTACGAGGTGGCCCAGTTTAATGTGGAACATTTCTCAGGGATGCAC
AACTGGTACGCCTGCTCCCACGCCCGACCCACCTTCTGTAACGTGTGCAGAGAGAGTCTT
TCTGGAGTCACCTCCCATGGCCTGTCCTGCGAAGTGTGTAAATTCAAGGCTCACAAAAGA
TGTGCAGTGAGAGCAACAAATAACTGTAAATGGACTACCCTGGCCTCCATCGGGAAGGAC
ATTATAGAAGATGAAGATGGCGTCGCGATGCCTCACCAGTGGCTTGAGGGCAACCTGCCT
GTAAGTGCCAAGTGTGCTGTCTGCGACAAAACATGTGGCAGTGTTCTCCGTCTACAGGAT
TGGAAATGCCTTTGGTGTAAGACAATGGTACACACTGCCTGCAAAGATTTATACCATCCA
ATATGTCCACTTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGCACC
GATTCCGATGGTTTCTGTAGAGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTT
GTCAATTCTAAGAGTGGAGATAATCAGGGAGTAAAGTTCCTCCGTCGCTTTAAACAGTTG
CTAAATCCGGCTCAGGTGTTTGATTTAATGAATGGAGGTCCTCATTTAGGTTTAAGATTA
TTTCAGAAGTTTGACAATTTCCGGATTCTTGTTTGTGGAGGCGATGGAAGTGTAGGTTGG
GTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAACAGTGTCAGCTGGGAGTGTTGCCT
TTGGGTACAGGAAATGACCTTGCACGAGTTCTTGGCTGGGGAGGTTCATATGACGATGAC
ACCCAACTTCCTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGACAGG
TGGAGTATAATGACATATGAACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCT
CCAGAAGCATCTGAAGAATTTTATATGACGATTTATGAAGACTCAGTTGCAACGCATCTT
ACAAAAATCCTCAATTCTGATGAACATGCAGTGGTCATATCTTCTGCCAAGACGCTATGT
GAAACTGTAAAGGACTTCGTTGCCAAAGTAGAAAAGACGTATGACAAAACCTTGGAAAAT
GCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAGTCCTAAACGAGAAGCTCGAACAA
CTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCCTGGCCTCAGCCCT
CTCATTGTGGAAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGCAAA
GAGCAGCTTGGGGATGACGTTACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAA
ATCATGTTGCGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAAGTCATTGAGGAAGCCGGA
AAAGTTATGGATGACCCGACAGTTCACCCGTGTGAACCAGCTAATCAGTCCTCTGATTAT
GACAGCACAGAAACAGATGAATCTAAGGAGGAAGCTAAAGATGATGGTGCCAAAGAATCA
ATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCCCGGGCAAGTTATGGCCATTCCCAA
ACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAACCTCCCTGTGCTCAAT
ACCAGAATAATCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGGAGTTCG
ATTATCAACAAAATGTTACTGGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGAC
CCGGATCTAGATTCCGTAGATGGATATTCAGAAAAATGTGTCATGAACAATTACTTTGGG
ATTGGATTAGATGCAAAAATTTCATTAGAATTTAATAATAAAAGAGAGGAGCACCCTGAA
AAATGCAGGAGCCGAACTAAAAACTTGATGTGGTATGGAGTCCTTGGAACCCGGGAGTTA
TTACAGAGATCGTACAAGAATTTAGAACAAAGGGTTCAACTTGAGTGTGATGGGCAGTAT
ATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAACATTCCCAGCTATGCTGGAGGC
ACTAACTTTTGGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCATCCTTTGATGAC
AAGATCCTGGAAGTTGTAGCAATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTCATT
AAACTGCAGCATCATCGAATAGCCCAGTGCCGTACAGTGAAAATCACTATATTTGGTGAC
GAAGGAGTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGATTATCAAA
ATTGTGCACAAAAACAGAGCACAAATGCTAACAAGGGACAGAGCCTTTGAGAGCACTCTG
AAATCTTGGGAAGATAAGCAGAAGTGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTG
TACATCCATCACGCCATTGACTTGGCAACAGAAGAGGTGTCGCAGATGCAGCTATGCTCC
CAGGCTGCAGAGGAGCTCATTACTAGGATATGTGACGCAGCCACAATTCACTGTCTTTTG
GAGCAAGAACTGGCCCATGCTGTGAATGCCTGCTCCCATGCCCTGAATAAAGCCAACCCA
AGGTGCCCGGAGAGTCTTACAAGAGACACTGCCACTGAAATAGCCATCAATGTGAAGGCG
CTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTGGAATCGCCA
CATGAAGAGCGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACAGAAACTGACA
GAGATTCCTTGGCTTTATTATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGAT
TGCACCAAAAGGAACAACAGAAGCACCGTATTTCGAATAGTGCCAAAGTTTAAAAAGGAA
AAGGTTCAGAAGCAGAAGACAAGTTCACAGCCTGTTCAGAAATGGGGCACAGAGGAAGTT
GCTGCTTGGCTGGATCTGCTCAATTTGGGAGAGTACAAAGATATCTTCATCCGTCATGAC
ATCAGAGGGGCTGAACTTTTGCATCTGGAAAGGCGAGATCTTAAGAACACAGTAGGAGAA
AAAAGAGACACCAAAGAAAATGGTAAACATATGGATCTGGGGATACCGAAAGTGGGTCAT
GTGAAGCGAATTCTCCAGGGAATTAAAGAGCTTGGAAGGAGCACTCCACAGTCGGAGGTG
TTG

Protein sequence predicted based on nucleotide sequence

MLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESL
SGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLP
VSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNST
DSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRL
FQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDD
TQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHL
TKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQ
LLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPRE
IMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKES
ITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSS
IINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPE
KCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGG
TNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGD
EGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHL
YIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANP
RCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLT
EIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEV
AAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKNTVGEKRDTKENGKHMDLGIPKVGH
VKRILQGIKELGRSTPQSEVL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3392
Start on reference sequence 90
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable