Gene:
Gene Symbol:
TEX14
Gene Name:
testis expressed 14
Original Clone ID:
FLH203945.01F
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00021524
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCTCGGGCTGTTCGTCTTCCAGTCCCCTGTCCTGTTCAACTTGGTACCTTAAGAAAT
GACTCCCTGGAAGCTCAGCTTCATGAGTATGTCAAACAAGGGAACTATGTGAAAGTGAAG
AAAATTCTTAAGAAAGGAATTTATGTTGATGCAGTTAACTCCTTGGGCCAAACAGCACTT
TTTGTTGCGGCGTTATTGGGCCTTAGGAAATTCGTTGATGTTCTGGTGGATTATGGATCA
GATCCAAATCACCGCTGCTTTGATGGGAGCACCCCTGTCCATGCAGCAGCATTTTCGGGC
AATCAGTGGATCCTTAGCAAACTGCTGGATGCAGGAGGTGACCTGCGACTCCACGATGAG
AGGGGTCAAAACCCGAAGACTTGGGCTTTGACAGCAGGAAAGGAGCGTAGCACCCAGATA
GTGGAGTTCATGCAGCGCTGTGCCTCACACATGCAGGCCATCATCCAGGGCTTCTCTTAC
GACCTCCTGAAGAAGATAGACTCCCCGCAGCGGCTTGTCTACAGCCCGTCCTGGTGTGGG
GGCCTCGTGCAGGGAAACCCTAATGGCTCTCCTAACCGACTGCTTAAAGCTGGAGTCATT
TCTGCTCAAAATATCTACAGCTTTGGTTTTGGGAAGGCTATGCCTTGGTTTCAGTTTTAT
CTTACTGGGGCGACACAGATGGCCTATCTAGGATCTCTTCCGGTCATTGGAGAAAAGGAA
GTGATTCAAGCTGATGATGAGCCCACCTTCTCTTTCTTCAGCGGCCCCTACATGGTCATG
ACCAACCTAGTGTGGAATGGGAGCAGGGTCACAGTGAAAGAGCTGAATCTCCCCACCCAC
CCACACTGCAGCAGGCTGCGGCTGGCCGACTTGTTAATTGCCGAGCAGGAACACAGCAGC
AAGCTGCGGCACCCCTACTTGCTACAGTTGATGGCTGTGTGTCTCTCCCAGGACCTAGAG
AAAACCCGCCTTGTGTACGAGCGCATCACTATCGGCACATTGTTCAGTGTCCTTCATGAA
CGACGGTCCCAGTTCCCAGTGCTGCACATGGAGGTGATTGTGCACCTGCTGCTCCAGATA
TCTGATGCCCTGAGATACCTGCATTTCCAGGGGTTTATCCACCGCTCCCTCAGCTCCTAT
GCTGTCCATATCATCTCCCCTGGTGAAGCGAGGCTGACCAACCTGGAGTACATGTTGGAA
AGCGAGGACAGAGGTGTACAGAGGGACCTGACTCGAGTGCCCCTTCCTACGCAGCTATAC
AACTGGGCCGCACCAGAAGTGATCTTACAGAAGGCAGCCACAGTGAAATCAGACATCTAC
AGCTTTTCTATGATCATGCAGGAGATTTTAACAGATGACATACCCTGGAAGGGCTTAGAT
GGCTCAGTTGTTAAAAAAGCCGTAGTCTCGGGGAATTATTTAGAAGCTGATGTCAGGCTT
CCGAAACCTTACTATGATATTGTTAAGTCAGGCATCCACGTCAAGCAGAAAGACCGAACT
ATGAACCTTCAAGATATCCGGTATATTCTGAAGAATGACTTAAAGGATTTTACTGGAGCC
CAGAGAACTCAACCAACCGAGAGCCCCAGAGTGCAGAGATACGGACTCCATCCCGATGTC
AATGTCTATCTAGGACTGACTTCAGAACACCCCAGAGAGACACCTGACATGGAAATCATA
GAACTAAAGGAAATGGGCAGTCAACCTCATTCACCAAGGGTTCACTCTTTATTCACTGAG
GGGACACTAGATCCTCAGGCCCCAGATCCATGTCTGATGGCCAGGGAGACTCAGAATCAA
GATGCTCCTTGCCCTGCTCCATTTATGGCAGAAGAGGCCAGCAGCCCCAGCACAGGTCAG
CCAAGCCTCTGCAGTTTCGAAATCAACGAGATCTACTCAGGCTGCTTGATTTTGGAAGAT
GACATAGAAGAGCCTCCAGGAGCTGCTTCATCTTTGGAGGCAGACGGACCTAACCAGGTA
GATGAACTGAAATCCATGGAAGAAGAGCTGGATAAGATGGAGAGAGAGGCGTGTTGTTTT
GGCAGTGAGGATGAGAGCTCTTCAAAAGCTGAGACAGAGTACTCTTTTGATGACTGGGAC
TGGCAAAACGGTTCACTCAGTTCACTCAGCCTTCCTGAGTCAACCAGAGAAGCCAAGAGC
AATTTGAACAACATGTCCACGACTGAGGAGTATCTCATCAGTAAGTGTGTGCTGGATCTA
AAGATTATGCAGACAATAATGCACGAGAATGATGATAGGCTGAGGAATATCGAGCAGATA
TTAGATGAAGTCGAGATGAAACAGAAGGAACAGGAAGAGCGCATGTCTTTATGGGCCACT
TCAAGAGAGTTTACAAATGCCTACAAGTTACCTCTGGCCGTGGGCCCTCCATCTTTAAAC
TATATTCCTCCTGTCCTACAGCTTTCAGGGGGTCAGAAGCCAGACACCAGTGGCAACTAC
CCAACCCTACCAAGATTTCCAAGAATGCTGCCGACTCTTTGTGACCCTGGAAAACAGAAC
ACAGATGAACAATTTCAGTGCACTCAAGGAGCCAAGGACAGTTTGGAAACAAGCAGGATC
CAAAATACCAGTAGCCAGGGAAGACCTAGAGAGTCCACTGCCCAAGCCAAAGCCACACAG
TTTAATAGTGCACTCTTCACTCTGTCAAGCCACCGGCAGGGACCTTCTGCATCACCCAGC
TGTCACTGGGACTCTACCAGGATGAGTGTGGAACCTGTTTCTTCTGAAATCTATAATGCA
GAGTCCAGAAATAAAGATGATGGAAAGGTACACTTAAAATGGAAAATGGAGGTGAAAGAA
ATGGCAAAGAAAGCAGCTACTGGACAGCTCACAGTACCTCCTTGGCATCCTCAGAGTAGT
CTGACTTTAGAGAGCGAGGCTGAAAATGAGCCCGACGCCCTGCTGCAGCCCCCCATTAGG
AGCCCAGAAAACACGGATTGGCAGCGAGTTATTGAGTATCATAGGGAAAATGATGAGCCC
AGAGGAAATGGCAAGTTTGACAAGACGGGCAACAATGACTGTGACAGTGACCAGCATGGC
AGACAGCCCAGGCTTGGAAGCTTCACCAGTATCAGGCACCCATCTCCCAGACAAAAGGAG
CAACCAGAGCATAGTGAAGCCTTCCAAGCAAGTTCTGACACATTGGTGGCTGTAGAGAAA
TCTTACAGTCATCAGTCCATGCAATCAACTTGTTCACCAGAGTCTTCTGAGGATATAACA
GATGAATTTTTAACTCCAGACGGTGAATATTTTTACTCCTCGACTGCTCAAGAAAACTTA
GCTCTAGAGACCTCGAGTCCCATAGAAGAGGACTTTGAAGGAATACAAGGTGCATTTGCC
CAACCTCAAGTCTCTGGTGAGGAAAAGTTCCAAATGAGAAAAATTCTTGGAAAGAATGCT
GAGATTTTGCCCAGGTCTCAATTTCAACCTGTACGAAGTACTGAAGATGAACAAGAAGAG
ACATCAAAGGAGTCACCAAAGGAACTGAAAGAGAAAGACATATCATTGACGGATATTCAA
GACCTGTCTAGTATCTCCTATGAACCAGACAGCTCTTTTAAGGAAGCTTCATGCAAAACA
CCCAAAATAAACCATGCACCTACCAGTGTCAGCACTCCACTCAGCCCAGGGTCCGTTTCT
TCAGCTGCCAGTCAGTATAAAGACTGCCTTGAAAGTATCACATTTCAGGTTAAGACAGAG
TTTGCCTCTTGCTGGAACAGTCAAGAATTTATTCAAACTTTGTCTGATGACTTTATAAGT
GTCCGAGAGAGAGCAAAGAAACTGGATTCTCTCCTTACTTCCTCTGAAACTCCCCCTTCA
AGACTGACTGGTCTTAAAAGATTGTCTTCATTTATTGGGGCTGGATCCCCCAGCCTTGTT
AAGGCATGTGACTCATCACCACCCCATGCCACCCAGAGAAGGAGCCTGCCTAAAGTAGAA
GCCTTCTCACAGCATCACATTGATGAGCTGCCACCACCATCTCAGGAGCTACTTGATGAC
ATTGAGCTCTTGAAACAGCAGCAGGGCTCATCCACGGTGTTGCATGAGAACACAGCAAGT
GATGGAGGAGGCACTGCAAATGATCAAAGGCACTTAGAAGAACAAGAAACTGACAGTAAA
AAAGAAGATAGTAGTATGCCTTTGTCCAAAGAAACTGAAGATCTTGGAGAGGACACAGAG
AGAGCTCACTCTACTCTGGATGAGGACCTGGAAAGATGGCTGCAGCCACCTGAGGAGAGC
GTGGAGCTACAAGACCTTCCCAAGGGCTCTGAAAGGGAGACAAATATCAAAGATCAAAAA
GTTGGTGAAGAGAAAAGAAAAAGGGAAGATAGCATTACACCAGAGAGAAGGAAATCAGAG
GGTGTTCTAGGGACTTCTGAAGAAGATGAACTAAAATCCTGTTTTTGGAAGCGACTAGGT
TGGTCCGAATCATCCAGGATAATCGTGCTGGATCAGAGTGACTTGTCAGACTTG
Protein sequence predicted based on nucleotide sequence
MSRAVRLPVPCPVQLGTLRNDSLEAQLHEYVKQGNYVKVKKILKKGIYVDAVNSLGQTAL FVAALLGLRKFVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLDAGGDLRLHDE RGQNPKTWALTAGKERSTQIVEFMQRCASHMQAIIQGFSYDLLKKIDSPQRLVYSPSWCG GLVQGNPNGSPNRLLKAGVISAQNIYSFGFGKAMPWFQFYLTGATQMAYLGSLPVIGEKE VIQADDEPTFSFFSGPYMVMTNLVWNGSRVTVKELNLPTHPHCSRLRLADLLIAEQEHSS KLRHPYLLQLMAVCLSQDLEKTRLVYERITIGTLFSVLHERRSQFPVLHMEVIVHLLLQI SDALRYLHFQGFIHRSLSSYAVHIISPGEARLTNLEYMLESEDRGVQRDLTRVPLPTQLY NWAAPEVILQKAATVKSDIYSFSMIMQEILTDDIPWKGLDGSVVKKAVVSGNYLEADVRL PKPYYDIVKSGIHVKQKDRTMNLQDIRYILKNDLKDFTGAQRTQPTESPRVQRYGLHPDV NVYLGLTSEHPRETPDMEIIELKEMGSQPHSPRVHSLFTEGTLDPQAPDPCLMARETQNQ DAPCPAPFMAEEASSPSTGQPSLCSFEINEIYSGCLILEDDIEEPPGAASSLEADGPNQV DELKSMEEELDKMEREACCFGSEDESSSKAETEYSFDDWDWQNGSLSSLSLPESTREAKS NLNNMSTTEEYLISKCVLDLKIMQTIMHENDDRLRNIEQILDEVEMKQKEQEERMSLWAT SREFTNAYKLPLAVGPPSLNYIPPVLQLSGGQKPDTSGNYPTLPRFPRMLPTLCDPGKQN TDEQFQCTQGAKDSLETSRIQNTSSQGRPRESTAQAKATQFNSALFTLSSHRQGPSASPS CHWDSTRMSVEPVSSEIYNAESRNKDDGKVHLKWKMEVKEMAKKAATGQLTVPPWHPQSS LTLESEAENEPDALLQPPIRSPENTDWQRVIEYHRENDEPRGNGKFDKTGNNDCDSDQHG RQPRLGSFTSIRHPSPRQKEQPEHSEAFQASSDTLVAVEKSYSHQSMQSTCSPESSEDIT DEFLTPDGEYFYSSTAQENLALETSSPIEEDFEGIQGAFAQPQVSGEEKFQMRKILGKNA EILPRSQFQPVRSTEDEQEETSKESPKELKEKDISLTDIQDLSSISYEPDSSFKEASCKT PKINHAPTSVSTPLSPGSVSSAASQYKDCLESITFQVKTEFASCWNSQEFIQTLSDDFIS VRERAKKLDSLLTSSETPPSRLTGLKRLSSFIGAGSPSLVKACDSSPPHATQRRSLPKVE AFSQHHIDELPPPSQELLDDIELLKQQQGSSTVLHENTASDGGGTANDQRHLEEQETDSK KEDSSMPLSKETEDLGEDTERAHSTLDEDLERWLQPPEESVELQDLPKGSERETNIKDQK VGEEKRKREDSITPERRKSEGVLGTSEEDELKSCFWKRLGWSESSRIIVLDQSDLSDL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4580
Start on reference sequence
87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDNR-Dual
Synonyms:
''
Sequencing Primer:
Forward:
M13F
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Creator™, donor (entry), loxP, multiple cloning site, recombinational cloning clone with LoxP and 6X His and T7 Terminator and CmR and sopB and sacB and AmpR promoter and AmpR and ori and M13 forward and T7 promoter; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp):
4938
Parent Vector:
None
Properties:
Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
6X His
6x(His-Asn) affinity tag
141
176
CDS
CmR
confers resistance to chloramphenicol
327
986
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
3103
3963
CDS
sacB
secreted levansucrase that renders bacterial growth sensitive to sucrose
1509
2930
Terminator
T7 Terminator
transcription terminator from phage lambda
212
306
primer site
T7 promoter
promoter for bacteriophage T7 RNA polymerase
4863
4881
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
4839
4855
promoter
sopB
sacB promoter and control region
1063
1508
promoter
AmpR promoter
null
2998
3102
protein binding site
LoxP
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
9
42
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4134
4722
