Gene:
Gene Symbol:
PTPRF
Gene Name:
protein tyrosine phosphatase, receptor type, F
Original Clone ID:
FLH159229.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00021632
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCCCTGAGCCAGCCCCAGGGAGGACGATGGTGCCCCTTGTGCCTGCACTGGTGATG
CTTGGTTTGGTGGCAGGCGCCCATGGTGACAGCAAACCTGTCTTCATTAAAGTCCCTGAG
GACCAGACTGGGCTGTCAGGAGGGGTAGCCTCCTTCGTGTGCCAAGCTACAGGAGAACCC
AAGCCGCGCATCACATGGATGAAGAAGGGGAAGAAAGTCAGCTCCCAGCGCTTCGAGGTC
ATTGAGTTTGATGATGGGGCAGGGTCAGTGCTTCGGATCCAGCCATTGCGGGTGCAGCGA
GATGAAGCCATCTATGAGTGTACAGCTACTAACAGCCTGGGTGAGATCAACACTAGTGCC
AAGCTCTCAGTGCTCGAAGAGGAACAGCTGCCCCCTGGGTTCCCTTCCATCGACATGGGG
CCTCAGCTGAAGGTGGTGGAGAAGGCACGCACAGCCACCATGCTATGTGCCGCAGGCGGA
AATCCAGACCCTGAGATTTCTTGGTTCAAGGACTTCCTTCCTGTAGACCCTGCCACGAGC
AACGGCCGCATCAAGCAGCTGCGTTCAGGTGCCTTGCAGATAGAGAGCAGTGAGGAATCC
GACCAAGGCAAGTACGAGTGTGTGGCGACCAACTCGGCAGGCACACGTTACTCAGCCCCT
GCGAACCTGTATGTGCGAGTGCGCCGCGTGGCTCCTCGTTTCTCCATCCCTCCCAGCAGC
CAGGAGGTGATGCCAGGCGGCAGCGTGAACCTGACATGCGTGGCAGTGGGTGCACCCATG
CCCTACGTGAAGTGGATGATGGGGGCCGAGGAGCTCACCAAGGAGGATGAGATGCCAGTT
GGCCGCAACGTCCTGGAGCTCAGCAATGTCGTACGCTCTGCCAACTACACCTGTGTGGCC
ATCTCCTCGCTGGGCATGATCGAGGCCACAGCCCAGGTCACAGTGAAAGCTCTTCCAAAG
CCTCCGATTGATCTTGTGGTGACAGAGACAACTGCCACCAGTGTCACCCTCACCTGGGAC
TCTGGGAACTCGGAGCCTGTAACCTACTATGGCATCCAGTACCGCGCAGCGGGCACGGAG
GGCCCCTTTCAGGAGGTGGATGGTGTGGCCACCACCCGCTACAGCATTGGCGGCCTCAGC
CCTTTCTCGGAATATGCCTTCCGCGTGCTGGCGGTGAACAGCATCGGGCGAGGGCCGCCC
AGCGAGGCAGTGCGGGCACGCACGGGAGAACAGGCGCCCTCCAGCCCACCGCGCCGCGTG
CAGGCACGCATGCTGAGCGCCAGCACCATGCTGGTGCAGTGGGAGCCTCCCGAGGAGCCC
AACGGCCTGGTGCGGGGATACCGCGTCTACTATACTCCGGACTCCCGCCGCCCCCCGAAC
GCCTGGCACAAGCACAACACCGACGCGGGGCTCCTCACGACCGTGGGCAGCCTGCTGCCT
GGCATCACCTACAGCCTGCGCGTGCTTGCCTTCACCGCCGTGGGCGATGGCCCTCCCAGC
CCCACCATCCAGGTCAAGACGCAGCAGGGAGTGCCTGCCCAGCCCGCGGACTTCCAGGCC
GAGGTGGAGTCGGACACCAGGATCCAGCTCTCGTGGCTGCTGCCCCCTCAGGAGCGGATC
ATCATGTATGAACTGGTGTACTGGGCGGCAGAGGACGAAGACCAACAGCACAAGGTGACC
TTCGACCCAACCTCCTCCTACACACTAGAGGACCTGAAGCCTGACACACTCTACCGCTTC
CAGCTGGCTGCACGCTCGGATATGGGGGTGGGCGTCTTCACCCCCACCATTGAGGCCCGC
ACAGCCCAGTCCACCCCCTCCGCCCCTCCCCAGAAGGTGATGTGTGTGAGCATGGGCTCC
ACCACGGTCCGGGTAAGTTGGGTCCCGCCGCCTGCCGACAGCCGCAACGGCGTTATCACC
CAGTACTCCGTGGCCTACGAGGCGGTGGACGGCGAGGACCGCGGGCGGCATGTGGTGGAT
GGCATCAGCCGTGAGCACTCCAGCTGGGACCTGGTGGGCCTGGAGAAGTGGACGGAGTAC
CGGGTGTGGGTGCGGGCACACACAGACGTGGGCCCCGGCCCCGAGAGCAGCCCGGTGCTG
GTGCGCACCGATGAGGACGTGCCCAGCGGGCCTCCGCGGAAGGTGGAGGTGGAGCCACTG
AACTCCACTGCTGTGCATGTCTACTGGAAGCTGCCTGTCCCCAGCAAGCAGCATGGCCAG
ATCCGCGGCTACCAGGTCACCTACGTGCGGCTGGAGAATGGCGAGCCCCGTGGACTCCCC
ATCATCCAAGACGTCATGCTAGCCGAGGCCCAGGAAACCACTATCAGCGGCCTGACCCCG
GAGACCACCTACTCCGTTACTGTTGCTGCCTATACCACCAAGGGGGATGGTGCCCGCAGC
AAGCCCAAAATTGTCACTACAACAGGTGCAGTCCCAGGCCGGCCCACCATGATGATCAGC
ACCACGGCCATGAACACTGCGCTGCTCCAGTGGCACCCACCCAAGGAACTGCCTGGCGAG
CTGCTGGGCTACCGGCTGCAGTACTGCCGGGCCGACGAGGCGCGGCCCAACACCATAGAT
TTCGGCAAGGATGACCAGCACTTCACAGTCACCGGCCTGCACAAGGGGACCACCTACATC
TTCCGGCTTGCTGCCAAGAACCGGGCTGGCTTGGGTGAGGAGTTCGAGAAGGAGATCAGG
ACCCCCGAGGACCTGCCCAGCGGCTTCCCCCAAAACCTGCATGTGACAGGACTGACCACG
TCTACCACAGAACTGGCCTGGGACCCGCCAGTGCTGGCGGAGAGGAACGGGCGCATCATC
AGCTACACCGTGGTGTTCCGAGACATCAACAGCCAACAGGAGCTGCAGAACATCACGACA
GACACCCGCTTTACCCTTACTGGCCTCAAGCCAGACACCACTTACGACATCAAGGTCCGC
GCATGGACCAGCAAAGGCTCTGGCCCACTCAGCCCCAGCATCCAGTCCCGGACCATGCCG
GTGGAGCAAGTGTTTGCCAAGAACTTCCGGGTGGCGGCTGCAATGAAGACGTCTGTGCTG
CTCAGCTGGGAGGTTCCCGACTCCTATAAGTCAGCTGTGCCCTTTAAGATTCTGTACAAT
GGGCAGAGTGTGGAGGTGGACGGGCACTCGATGCGGAAGCTGATCGCAGACCTGCAGCCC
AACACAGAGTACTCGTTTGTGCTGATGAACCGTGGCAGCAGCGCAGGGGGCCTGCAGCAC
CTGGTGTCCATCCGCACAGCCCCCGACCTCCTGCCTCACAAGCCGCTGCCTGCCTCTGCC
TACATAGAGGACGGCCGCTTCGATCTCTCCATGCCCCATGTGCAAGACCCCTCGCTTGTC
AGGTGGTTCTACATTGTTGTGGTGCCCATTGACCGTGTGGGCGGGAGCATGCTGACGCCA
AGGTGGAGCACACCCGAGGAACTGGAGCTGGACGAGCTTCTAGAAGCCATCGAGCAAGGC
GGAGAGGAGCAGCGGCGGCGGCGGCGGCAGGCAGAACGTCTGAAGCCATATGTGGCTGCT
CAACTGGATGTGCTCCCGGAGACCTTTACCTTGGGGGACAAGAAGAACTACCGGGGCTTC
TACAACCGGCCCCTGTCTCCGGACTTGAGCTACCAGTGCTTTGTGCTTGCCTCCTTGAAG
GAACCCATGGACCAGAAGCGCTATGCCTCCAGCCCCTACTCGGATGAGATCGTGGTCCAG
GTGACACCAGCCCAGCAGCAGGAGGAGCCGGAGATGCTGTGGGTGACGGGTCCCGTGCTG
GCAGTCATCCTCATCATCCTCATTGTCATCGCCATCCTCTTGTTCAAAAGGAAAAGGACC
CACTCTCCGTCCTCTAAGGATGAGCAGTCGATCGGACTGAAGGACTCCTTGCTGGCCCAC
TCCTCTGACCCTGTGGAGATGCGGAGGCTCAACTACCAGACCCCAGGTATGCGAGACCAC
CCACCCATCCCCATCACCGACCTGGCGGACAACATCGAGCGCCTCAAAGCCAACGATGGC
CTCAAGTTCTCCCAGGAGTATGAGTCCATCGACCCTGGACAGCAGTTCACGTGGGAGAAT
TCAAACCTGGAGGTGAACAAGCCCAAGAACCGCTATGCGAATGTCATCGCCTACGACCAC
TCTCGAGTCATCCTTACCTCTATCGATGGCGTCCCCGGGAGTGACTACATCAATGCCAAC
TACATCGATGGCTACCGCAAGCAGAATGCCTACACCGCCACGCAGGGCCCCCTGCCCGAG
ACCATGGGTGATTTCTGGAGGATGGTGTGGGAACAGCGCACGGCCACTGTGGTCATGATG
ACACGGCTGGAGGAGAAGTCCCGGGTAAAATGTGATCAGTACTGGCCAGCCCGTGGCACC
GAGACCTGTGGCCTTATTCAGGTGACCCTGTTGGACACAGTGGAGCTGGCCACATACACT
GTGCGCACCTTCGCACTCCACAAGAGTGGCTCCAGTGAGAAGCGCGAGCTGCGTCAGTTT
CAGTTCATGGCCTGGCCAGACCATGGAGTTCCTGAGTACCCAACTCCCATCCTGGCCTTC
CTACGACGGGTCAAGGCCTGCAACCCCCTAGACGCAGGGCCCATGGTGGTGCACTGCAGC
GCGGGCGTGGGCCGCACCGGCTGCTTCATCGTGATTGATGCCATGTTGGAGCGGATGAAG
CACGAGAAGACGGTGGACATCTATGGCCACGTGACCTGCATGCGATCACAGAGGAACTAC
ATGGTGCAGACGGAGGACCAGTACGTGTTCATCCATGAGGCGCTGCTGGAGGCTGCCACG
TGCGGCCACACAGAGGTGCCTGCCCGCAACCTGTATGCCCACATCCAGAAGCTGGGCCAA
GTGCCTCCAGGGGAGAGTGTGACCGCCATGGAGCTCGAGTTCAAGTTGCTGGCCAGCTCC
AAGGCCCACACGTCCCGCTTCATCAGCGCCAACCTGCCCTGCAACAAGTTCAAGAACCGG
CTGGTGAACATCATGCCCTACGAATTGACCCGTGTGTGTCTGCAGCCCATCCGTGGTGTG
GAGGGCTCTGACTACATCAATGCCAGCTTCCTGGATGGTTATAGACAGCAGAAGGCCTAC
ATAGCTACACAGGGGCCTCTGGCAGAGAGCACCGAGGACTTCTGGCGCATGCTATGGGAG
CACAATTCCACCATCATCGTCATGCTGACCAAGCTTCGGGAGATGGGCAGGGAGAAATGC
CACCAGTACTGGCCAGCAGAGCGCTCTGCTCGCTACCAGTACTTTGTTGTTGACCCGATG
GCTGAGTACAACATGCCCCAGTATATCCTGCGTGAGTTCAAGGTCACGGATGCCCGGGAT
GGGCAGTCAAGGACAATCCGGCAGTTCCAGTTCACAGACTGGCCAGAGCAGGGCGTGCCC
AAGACAGGCGAGGGATTCATTGACTTCATCGGGCAGGTGCATAAGACCAAGGAGCAGTTT
GGACAGGATGGGCCTATCACGGTGCACTGCAGTGCTGGCGTGGGCCGCACCGGGGTGTTC
ATCACTCTGAGCATCGTCCTGGAGCGCATGCGCTACGAGGGCGTGGTCGACATGTTTCAG
ACCGTGAAGACCCTGCGTACACAGCGTCCTGCCATGGTGCAGACAGAGGACCAGTATCAG
CTGTGCTACCGTGCGGCCCTGGAGTACCTCGGCAGCTTTGACCACTATGCAACGTAG
Protein sequence predicted based on nucleotide sequence
MAPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFIKVPEDQTGLSGGVASFVCQATGEP KPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSA KLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATS NGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSS QEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVA ISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVTYYGIQYRAAGTE GPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRV QARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPPNAWHKHNTDAGLLTTVGSLLP GITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVESDTRIQLSWLLPPQERI IMYELVYWAAEDEDQQHKVTFDPTSSYTLEDLKPDTLYRFQLAARSDMGVGVFTPTIEAR TAQSTPSAPPQKVMCVSMGSTTVRVSWVPPPADSRNGVITQYSVAYEAVDGEDRGRHVVD GISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPL NSTAVHVYWKLPVPSKQHGQIRGYQVTYVRLENGEPRGLPIIQDVMLAEAQETTISGLTP ETTYSVTVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMISTTAMNTALLQWHPPKELPGE LLGYRLQYCRADEARPNTIDFGKDDQHFTVTGLHKGTTYIFRLAAKNRAGLGEEFEKEIR TPEDLPSGFPQNLHVTGLTTSTTELAWDPPVLAERNGRIISYTVVFRDINSQQELQNITT DTRFTLTGLKPDTTYDIKVRAWTSKGSGPLSPSIQSRTMPVEQVFAKNFRVAAAMKTSVL LSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQH LVSIRTAPDLLPHKPLPASAYIEDGRFDLSMPHVQDPSLVRWFYIVVVPIDRVGGSMLTP RWSTPEELELDELLEAIEQGGEEQRRRRRQAERLKPYVAAQLDVLPETFTLGDKKNYRGF YNRPLSPDLSYQCFVLASLKEPMDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVL AVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDH PPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDH SRVILTSIDGVPGSDYINANYIDGYRKQNAYTATQGPLPETMGDFWRMVWEQRTATVVMM TRLEEKSRVKCDQYWPARGTETCGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQF QFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMK HEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARNLYAHIQKLGQ VPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGV EGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKC HQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVP KTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQ TVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
327
End on reference sequence
6023
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDNR-Dual
Synonyms:
''
Sequencing Primer:
Forward:
M13F
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Creator™, donor (entry), loxP, multiple cloning site, recombinational cloning clone with LoxP and 6X His and T7 Terminator and CmR and sopB and sacB and AmpR promoter and AmpR and ori and M13 forward and T7 promoter; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp):
4938
Parent Vector:
None
Properties:
Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
6X His
6x(His-Asn) affinity tag
141
176
CDS
CmR
confers resistance to chloramphenicol
327
986
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
3103
3963
CDS
sacB
secreted levansucrase that renders bacterial growth sensitive to sucrose
1509
2930
Terminator
T7 Terminator
transcription terminator from phage lambda
212
306
primer site
T7 promoter
promoter for bacteriophage T7 RNA polymerase
4863
4881
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
4839
4855
promoter
sopB
sacB promoter and control region
1063
1508
promoter
AmpR promoter
null
2998
3102
protein binding site
LoxP
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
9
42
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4134
4722
