Gene:
Gene Symbol:
PHKA1
Gene Name:
phosphorylase kinase, alpha 1 (muscle)
Original Clone ID:
FLH173530.01F
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors:
HIP
HsCD00022411
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAG
ACCATCCTGTGCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAA
GATGCTTGGGTCCGAGATAATGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCC
TATCGGAAGAATGCAGACCGGGATGAGGATAAGGCAAAGGCCTATGAATTGGAGCAGAGT
GTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCAGACAGGTGGATAAAGTAGAA
TCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAGTACAACACCAAAACC
TGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTG
TACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGAT
GAAGTCAATTTCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGAC
TTCGGGATATGGGAACGTGGAGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGT
TCAGTTGGAATGGCAAAGGCAGCCCTGGAAGCATTAGATGAACTGGATCTGTTTGGTGTG
AAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATGAAGTACAGCACTGCCAGTCT
ATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAGGTTGATGCTAGTCTACTC
TCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAA
CAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGA
TATAAAACTCCTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTA
TTTGAAAACATTGAGTGTGAATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTC
TTCAGTGGCAATGCAGAACAGGTTCAAGAATATAAAGAGGCTCTTGAAGCAGTCCTCATC
AAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACAGTGTTCCTCCTGACAGGGTC
GATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCCATGGGGAAATTGCCTCAC
ATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCT
GGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTT
CAAGTCTCCATTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATT
TACGTGGAGACCATTGCTGAGGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGC
CACATTTATTCCAGCCTAGGATGCAACAATAGAATGAAACTCAGTGGACGACCCTACAGA
CACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTCGGAAAACTATCTTTACTTTC
ACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACAAGATGATAGTG
GAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCC
ACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCA
AGTATCCTGGCAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAA
ACAGGTAAATTGTCAGAGTTTTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGAC
CCTGGACCTGAGGGTAAGCTGTACAGTGAAGATTATGATGACAACTATGATTACCTGGAA
TCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATGCTCGCTGTGGTGATGAACTT
GCTCGTTATTTACATCACCTTTTGGCGCACACTGCTCCCCATCCTAAACTAGCCCCTACC
TCACAGAAGGGAGGGCTAGATCGGTTCCAAGCTGCTGTGCAAACAACCTGCGACTTAATG
TCCTTGGTGACCAAGGCCAAGGAACTGCATGTACAGAATGTTCACATGTATCTTCCTACG
AAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACCTCATCCCCGACAG
GAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGGGAG
GTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCAAGCTTACAGGAACAAGCT
GATATCCTCTATATGCTGTATACTATGAAAGGACCTGACTGGAACACTGAATTGTATAAT
GAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTATGGCAAAGTGGGAGAAATT
CGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCACTT
GATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTGACAGTAGGACTTCCTCCA
GAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAGGCGCTCACTCAGCTGATA
GATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACACAGGAAATAATGGTATAT
CTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAAATGTTTCGACTTCGAATT
GGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACTCCCTTCGATGCTCAGCTGAG
GAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATGAAGAATCTCCTGCATCAC
ATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTC
AGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCAACCAAAACAGAACGAACT
GGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCACCTGGAACCTCTATGACTCCAAGT
AGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAAGATAGTCGTCAAGGTCAA
TGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTTCCAGTTGGATTTTATCAG
AAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTTGAAGGGTTTGTCCTTCCT
TCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTCTCTGTTCATGTGGAGTCT
GTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTGGTTGAAGCCATCCTTGTC
CTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGCATCATTGCTGTGGAAAAA
ATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGAAAACCCTTGGCGCAGATGAT
ACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCACCC
AGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCCGCCACCTACGTGCAGGAG
TTCCTGCCCCACAGCATCTGTGCCATGCAATTG
Protein sequence predicted based on nucleotide sequence
MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLA YRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKT CATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTAD FGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQS ILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTKQEIITKLQGRYGCCRFLRDG YKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYKEALEAVLI KGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILS HIYSSLGCNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIV EMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQ TGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDNYDYLESGNWMNDYDSTSHARCGDEL ARYLHHLLAHTAPHPKLAPTSQKGGLDRFQAAVQTTCDLMSLVTKAKELHVQNVHMYLPT KLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSGEVDFKALVLQLKETSSLQEQA DILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEAL DEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVY LAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHH ILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPS SGSFPSAYDQQSSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVEGFVLP SSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEK IVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQE FLPHSICAMQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
162
End on reference sequence
3833
Mutations:
None
Discrepancy:
t1702c,Y568H;g1978c,V660L;
g1993c,D665H;del@3031,39;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDNR-Dual
Synonyms:
''
Sequencing Primer:
Forward:
M13F
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Creator™, donor (entry), loxP, multiple cloning site, recombinational cloning clone with LoxP and 6X His and T7 Terminator and CmR and sopB and sacB and AmpR promoter and AmpR and ori and M13 forward and T7 promoter; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp):
4938
Parent Vector:
None
Properties:
Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
6X His
6x(His-Asn) affinity tag
141
176
CDS
CmR
confers resistance to chloramphenicol
327
986
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
3103
3963
CDS
sacB
secreted levansucrase that renders bacterial growth sensitive to sucrose
1509
2930
Terminator
T7 Terminator
transcription terminator from phage lambda
212
306
primer site
T7 promoter
promoter for bacteriophage T7 RNA polymerase
4863
4881
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
4839
4855
promoter
sopB
sacB promoter and control region
1063
1508
promoter
AmpR promoter
null
2998
3102
protein binding site
LoxP
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
9
42
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4134
4722
