Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH000727.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR201
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00023346
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1578nts
Open reading frame: 1 to 1578

ATGATTTTCTCACTAGATGAAGAACTTCATCGTGTGTCACTAGATGATAAAAAGAATGAC
ATTAAAGTAGATTATTCCTCGGCAATATACAATGATATCAATCATGAACAAGGCAGCTCC
ATCACATATGAGGAGAGTATAAATCACCTCTCGGTTCATTCAAATGCGATTCCGCTAAAT
GGCATGAGTCCTGCACATAGGATGAGAAGAAGGTCCTCAGCTTATTCCAAATTCCCTATT
CTCACTCCACCAAATACGAGAAGATTTTCTATTACAGGTTCGGACGCAATGCGAACTAAT
ACGAATAGACTATCCATAACACCTCAGGACATTATTTCCTCTAATATTGGAGAAAATGAA
TTATCAAGGAACCTGCACGATTTTAAGCCCGTGAGAGTCCTAGGCCAAGGTGCCTACGGT
AAAGTTCTTCTTGTTAAGGACGTCAATACATCCAAGCTGTATGCTATGAAGCAATTACGG
AAAGCAGAAATTTTAATTTCTCAAACAGCCACAGATTCCAAGAGAGAAGACGAAGATAAA
AATGATGGTAATAACAACGATAATGACGATGGATTATCAAAGAGGCTTGAGAGAACATTT
GCCGAACGGTCTATTCTATCTGAAATAGAACATCCAAACATTGTCAAGTTATTTTATTCT
TTTCACGATAACTCAAAGTTATATTTACTGCTGCAATATATCCCTGGCGGTGAACTATTT
TACCACTTGAAGGAACACGGGACCCTAGATGAGACGACAGTTTCGTTTTACGCGGCAGAA
ATCAGCTGTGCCTTGAGATTTTTGCATACGAAAGGTGTTGTCTATAGAGATTTAAAACCT
GAAAACTGTTTATTGAATCAACGTGGACATTTAGTATTGACAGATTTTGGTCTGAGTAAA
AAAAGTGCTAATGATTCCGCGGTTGATGAGGAGGATCCAGAGAACGTCAATGCCCTATAC
TCGATTATCGGTACGCCTGAATACTGTGCGCCTGAAATATTATTAGGTAAAGCTTACAGT
CAGAATTGTGATTGGTATTCTTTGGGATGTCTGTTGTATGACATGTTAGTAGGTAAGCCT
CCATATACTAGTAGCAATCATAAGGTGATCATTAATAAGATTCAACAAAACAAGCAAGGC
CCCAAAATTCCATTTTATCTGAGCGAGGGAATGAAAGATATATTAAACGCGCTGTTGAAG
AAGGAAACTGCCAAAAGGTGGAATGTTGACAAATACTGGGCCAAAACCGGGGCTAATAAT
AAGCCTACTAAGTCTAAGAAGAAGAAATCAGGGGCTGCAAGAACTAGTCTTTTCACTGAA
CATTTTATCTTTAGAAAAATAGACTGGAAGTTATTAGAATCTGGACAATTGCAAAAAACC
ACTCTAGGCCCAATTGTTCCAGTTATTACGGATTTGGAACTAGCGGAAAATTTTGACACA
GAATTTACTTCTATGTCATATGAGGAAACCTATACGGATAGCAAGCCGATTAATATCAAT
TCGGTGAGCAAGTCCCCGGATATGTTCAAGGGATTTAGTTATAAAGCAAGTGGTAGCTAT
TTGGAGAAGTACTTTTGA

Protein sequence predicted based on nucleotide sequence

MIFSLDEELHRVSLDDKKNDIKVDYSSAIYNDINHEQGSSITYEESINHLSVHSNAIPLN
GMSPAHRMRRRSSAYSKFPILTPPNTRRFSITGSDAMRTNTNRLSITPQDIISSNIGENE
LSRNLHDFKPVRVLGQGAYGKVLLVKDVNTSKLYAMKQLRKAEILISQTATDSKREDEDK
NDGNNNDNDDGLSKRLERTFAERSILSEIEHPNIVKLFYSFHDNSKLYLLLQYIPGGELF
YHLKEHGTLDETTVSFYAAEISCALRFLHTKGVVYRDLKPENCLLNQRGHLVLTDFGLSK
KSANDSAVDEEDPENVNALYSIIGTPEYCAPEILLGKAYSQNCDWYSLGCLLYDMLVGKP
PYTSSNHKVIINKIQQNKQGPKIPFYLSEGMKDILNALLKKETAKRWNVDKYWAKTGANN
KPTKSKKKKSGAARTSLFTEHFIFRKIDWKLLESGQLQKTTLGPIVPVITDLELAENFDT
EFTSMSYEETYTDSKPININSVSKSPDMFKGFSYKASGSYLEKYF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1578
Mutations:
None
Discrepancy:
a693g; g1090a,G364S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable