NCKAP1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NCKAP1
Gene Name:  NCK-associated protein 1
Original Clone ID:
FLH110727.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors:
HIP
HsCD00002335
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3387nts
Open reading frame: 1 to 3387

ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC
AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGGCATGTGGAGAC
CCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAATCTGCTGTGAAATTCATA
GTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAACAGCTTGCACAACTACAG
AAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTACTTCACATTTGTAGATGTT
ATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATTGACGTTTGCCAAGTCTTC
TTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTAGATTTAATTATAACCTAT
ACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAGGCAATCATTGGATTATAC
AACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAATACCCACGCCTTGGCCAG
ATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAAGAATTTGTACCCCATAGC
AAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATATCCTCGAAGGAATCTTTCA
GCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGTGCACCTAGTACAATGCTT
AATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCTTTGGATGCAATGGAAAAG
TGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAATACTGACGCTACAGCACTG
AACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTT
TTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGT
ATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACATGCTGGTTCAATGCACAGA
GAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAA
CCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGAT
GAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAGAAGAGTGCAGACGACTTT
ATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGG
AAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTTTCTGGCTTTGATGCTGTT
GTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGAATCAATCATCATG
TCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTA
TTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAGGCATATACTAGTGTCTCA
AAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAGATGATGAATACAATAATT
TTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTGGAAACATCAGATCTCTCC
ATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCC
TCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTTTATGAGTTGCACG
CATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTAAT
ATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAA
CAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCCAAAACTATCAGTCAAGCA
GTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCA
GGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAACCTTGATAAATTGCACACT
GCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAACATGGTGGTATGGGAACAT
ACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATT
GTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTA
ACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTATGTGCAGATTGAT
ATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGA
GAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAAACTTTGTTACGACAAGTC
AGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAACTTACCTACAGAA
AATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCAGAAATGAGGTCATTATCA
GAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCA
TCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATG
AGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTGTT
GACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCA
CAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTTCTTGTAAGTTCAATTGAA
GATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAATGTGTATGAG
TTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCT
TCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGCCTGCCTTCTCATG
GTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCT
GCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCT
GCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCG
CTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGAAAT
AGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAATCTCCATTCCTTACAATG
GATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCATGCTGTCTAC
AAACAAAGTGTTACATCTTCTGCATAG

Protein sequence predicted based on nucleotide sequence

MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFI
VRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVF
FDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQ
MIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTML
NPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEV
FHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQ
PGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVR
KYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEV
FDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLS
IFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCN
MFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKP
GVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSI
VGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHG
EPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLS
ELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSV
DSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYE
LSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSP
AIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRN
RESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 272
End on reference sequence 3658
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable