TFP1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH112753.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00023712
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3216nts
Open reading frame: 1 to 3216

ATGGCTGGTGCAATTGAAAACGCTCGTAAGGAAATAAAAAGAATCTCATTAGAAGACCAT
GCTGAATCTGAATATGGTGCCATCTATTCTGTCTCTGGTCCGGTCGTCATTGCTGAAAAT
ATGATTGGTTGTGCCATGTACGAATTGGTCAAGGTCGGTCACGATAACCTGGTGGGTGAA
GTCATTAGAATTGACGGTGACAAGGCCACCATCCAAGTTTACGAAGAAACTGCAGGCCTT
ACGGTCGGTGACCCTGTTTTGAGAACAGGTAAGCCTCTGTCGGTAGAATTGGGTCCTGGT
CTGATGGAAACCATTTACGATGGTATTCAAAGACCTTTGAAGGCCATTAAGGAAGAATCG
CAATCGATTTATATCCCAAGAGGTATTGACACTCCAGCTTTGGATAGGACTATCAAGTGG
CAATTTACTCCGGGAAAGTTTCAAGTCGGCGATCATATTTCCGGTGGTGATATTTACGGT
TCCGTTTTTGAGAATTCGCTAATTTCAAGCCATAAGATTCTTTTGCCACCAAGATCAAGA
GGTACAATCACTTGGATTGCTCCAGCTGGTGAGTACACTTTGGATGAGAAGATTTTGGAA
GTTGAATTTGATGGCAAGAAGTCTGATTTCACTCTTTACCATACTTGGCCTGTTCGTGTT
CCAAGACCAGTTACTGAAAAGTTATCTGCTGACTATCCTTTGTTAACAGGTCAAAGAGTT
TTGGATGCTTTGTTTCCTTGTGTTCAAGGTGGTACGACATGTATTCCAGGTGCTTTTGGT
TGTGGTAAGACCGTTATCTCTCAATCTTTGTCCAAGTACTCCAATTCTGACGCCATTATC
TATGTCGGGTGCTTTGCCAAGGGTACCAATGTTTTAATGGCGGATGGGTCTATTGAATGT
ATTGAAAACATTGAGGTTGGTAATAAGGTCATGGGTAAAGATGGCAGACCTCGTGAGGTA
ATTAAATTGCCCAGAGGAAGAGAAACTATGTACAGCGTCGTGCAGAAAAGTCAGCACAGA
GCCCACAAAAGTGACTCAAGTCGTGAAGTGCCAGAATTACTCAAGTTTACGTGTAATGCG
ACCCATGAGTTGGTTGTTAGAACACCTCGTAGTGTCCGCCGTTTGTCTCGTACCATTAAG
GGTGTCGAATATTTTGAAGTTATTACTTTTGAGATGGGCCAAAAGAAAGCCCCCGACGGT
AGAATTGTTGAGCTTGTCAAGGAAGTTTCAAAGAGCTACCCAATATCTGAGGGGCCTGAG
AGAGCCAACGAATTAGTAGAATCCTATAGAAAGGCTTCAAATAAAGCTTATTTTGAGTGG
ACTATTGAGGCCAGAGATCTTTCTCTGTTGGGTTCCCATGTTCGTAAAGCTACCTACCAG
ACTTACGCTCCAATTCTTTATGAGAATGACCACTTTTTCGACTACATGCAAAAAAGTAAG
TTTCATCTCACCATTGAAGGTCCAAAAGTACTTGCTTATTTACTTGGTTTATGGATTGGT
GATGGATTGTCTGACAGGGCAACTTTTTCGGTTGATTCCAGAGATACTTCTTTGATGGAA
CGTGTTACTGAATATGCTGAAAAGTTGAATTTGTGCGCCGAGTATAAGGACAGAAAAGAA
CCACAAGTTGCCAAAACTGTTAATTTGTACTCTAAAGTTGTCAGAGGTAATGGTATTCGC
AATAATCTTAATACTGAGAATCCATTATGGGACGCTATTGTTGGCTTAGGATTCTTGAAG
GACGGTGTCAAAAATATTCCTTCTTTCTTGTCTACGGACAATATCGGTACTCGTGAAACA
TTTCTTGCTGGTCTAATTGATTCTGATGGCTATGTTACTGATGAGCATGGTATTAAAGCA
ACAATAAAGACAATTCATACTTCTGTCAGAGATGGTTTGGTTTCCCTTGCTCGTTCTTTA
GGCTTAGTAGTCTCGGTTAACGCAGAACCTGCTAAGGTTGACATGAATGGCACCAAACAT
AAAATTAGTTATGCTATTTATATGTCTGGTGGAGATGTTTTGCTTAACGTTCTTTCGAAG
TGTGCCGGCTCTAAAAAATTCAGGCCTGCTCCCGCCGCTGCTTTTGCACGTGAGTGCCGC
GGATTTTATTTCGAGTTACAAGAATTGAAGGAAGACGATTATTGTGGGATTACTTTATCT
GATGATTCTGATCATCAGTTTTTGCTTGCCAACCAGGTTGTCGTCCATAATTGCGGAGAA
AGAGGTAATGAAATGGCAGAAGTCTTGATGGAATTCCCAGAGTTATATACTGAAATGAGC
GGTACTAAAGAACCAATTATGAAGCGTACTACTTTGGTCGCTAATACATCTAACATGCCG
GTTGCAGCCAGAGAAGCTTCTATTTACACTGGTATCACTCTTGCAGAATACTTCAGAGAT
CAAGGTAAAAATGTTTCTATGATTGCAGACTCTTCTTCAAGATGGGCTGAAGCTTTGAGA
GAAATTTCTGGTCGTTTGGGTGAGATGCCTGCTGATCAAGGTTTCCCAGCTTATTTGGGT
GCTAAGTTGGCCTCCTTTTACGAAAGAGCCGGTAAAGCTGTTGCTTTAGGTTCCCCAGAT
CGTACTGGTTCCGTTTCCATCGTTGCTGCCGTTTCGCCAGCCGGTGGTGATTTCTCAGAT
CCTGTTACTACTGCTACATTGGGTATCACTCAAGTCTTTTGGGGTTTAGACAAGAAATTG
GCTCAAAGAAAGCATTTCCCATCTATCAACACATCTGTTTCTTACTCCAAATACACTAAT
GTCTTGAACAAGTTTTATGATTCCAATTACCCTGAATTTCCTGTTTTAAGAGATCGTATG
AAGGAAATTCTATCAAACGCTGAAGAATTAGAACAAGTTGTTCAATTAGTTGGTAAATCG
GCCTTGTCTGATAGTGATAAGATTACTTTGGATGTTGCCACTTTAATCAAGGAAGATTTC
TTGCAACAAAATGGTTACTCCACTTATGATGCTTTCTGTCCAATTTGGAAGACATTTGAT
ATGATGAGAGCCTTCATCTCGTATCATGACGAAGCTCAAAAAGCTGTTGCTAATGGTGCC
AACTGGTCAAAACTAGCTGACTCTACTGGTGACGTTAAGCATGCCGTTTCTTCATCTAAA
TTTTTTGAACCAAGCAGGGGTGAAAAGGAAGTCCATGGCGAATTCGAAAAATTGTTGAGC
ACTATGCAAGAAAGATTTGCTGAATCTACCGATTAA

Protein sequence predicted based on nucleotide sequence

MAGAIENARKEIKRISLEDHAESEYGAIYSVSGPVVIAENMIGCAMYELVKVGHDNLVGE
VIRIDGDKATIQVYEETAGLTVGDPVLRTGKPLSVELGPGLMETIYDGIQRPLKAIKEES
QSIYIPRGIDTPALDRTIKWQFTPGKFQVGDHISGGDIYGSVFENSLISSHKILLPPRSR
GTITWIAPAGEYTLDEKILEVEFDGKKSDFTLYHTWPVRVPRPVTEKLSADYPLLTGQRV
LDALFPCVQGGTTCIPGAFGCGKTVISQSLSKYSNSDAIIYVGCFAKGTNVLMADGSIEC
IENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNA
THELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPE
RANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSK
FHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKE
PQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRET
FLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKH
KISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYCGITLS
DDSDHQFLLANQVVVHNCGERGNEMAEVLMEFPELYTEMSGTKEPIMKRTTLVANTSNMP
VAAREASIYTGITLAEYFRDQGKNVSMIADSSSRWAEALREISGRLGEMPADQGFPAYLG
AKLASFYERAGKAVALGSPDRTGSVSIVAAVSPAGGDFSDPVTTATLGITQVFWGLDKKL
AQRKHFPSINTSVSYSKYTNVLNKFYDSNYPEFPVLRDRMKEILSNAEELEQVVQLVGKS
ALSDSDKITLDVATLIKEDFLQQNGYSTYDAFCPIWKTFDMMRAFISYHDEAQKAVANGA
NWSKLADSTGDVKHAVSSSKFFEPSRGEKEVHGEFEKLLSTMQERFAESTD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3216
Mutations:
None
Discrepancy:
a342g; a2144g,Y715C
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable