CSR2 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148556.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00024279
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3366nts
Open reading frame: 1 to 3366

ATGCAATCTACTGTCCCAATAGCAATTGCGAGCAACGGCAACAAGAGAGATGTCGTCCAG
AACGTTTCTGCAGGCGACGAGGGTGATATATTGCAAAGGCTAGCCCGTAACAGAGAGATG
ATTTCTACCTCCCTCTCGCCACAAAAATCGTCAGGATTTAGTGGTAGAAGGAGGTCATCT
AGTGTTAGGGACGCCCTTTCTTCTTTTTTTGGAACTGGTAATAGTCCAACATCTAGTATG
GACGATTATTCCAATTTGATGAACCGTAACTATTCTACCGCATCAACTGCCATGTGTAGG
GGGAACAGTTTTCCTTCAGATGTTGGCACTAAGGCTTACAATATTACGGGATCTTACCAA
CCGGATAGGCACAGGAATTCTGTTCCGTATACCACCATAGACCAGTTACACACAAGGCAA
GACACCGGGTTGAGGAGAGAGTCAGATCCAGTCGCGGCAAAACAAATATCTAGTAATAAT
GATATTGTTAGGTCATTCATAACACATCATGCTAGTAATAGCACTATGTTTATTAATAGG
GTGCTATCGGACTATTTAGCGGATCGTGGTTTCATCAAGCAAACACCGTTATATAATAAG
AAAAGTGTGCTGGAAATTTCCATTGCAACAAGTGCAGAATCAGTTTTCTTGCCAACTACA
AAAAGTGATGAAACAGAGTATCTATCACTGATTCATGGTTCTTTAAATCAGGCGCGAACA
CAGCCTGTCGGGTCCACTAACACTGCAGAAAGTGATTTCCTCCCTTCATGTCCTACGATG
GACACTTTGAATGAAAACAATGATTTATCACTATTTCCGTTACATACACAGCGAACCAGC
CCCTCTAATACAGCAAGGACAGGTAATGCTATGGATACAAGCAATTCTGATCGTGCTTCT
CCGGCCTCGAACAATAACACAACAGATGCGGATTCCTTTGTTGCGAGTGGCAATAACAAC
CCTATGAATAATAACAATTCTCCCGCGAGAAACAGACATCCTAATTCTCACTCGCGTTCT
CTTCCTAATGCTTGGAATAGCCAAATGCCTTCATTCAGCTTTGCATTGATATTTTCCCTG
AATAAATCAACTACCCTTTCTGATATTAAAGTAGAACTCACTTCTAACGTGAGAGTGGTG
TGGTTCAATGGTCTTCCCCCTACCAAGAATGTTAACGAGGAATGCTATAATATAGGTTCT
TTGGACTGGACCTTAAATGCAGACAACTTTAATCTTTTCATTCCGCAAGGCGCAAAGTCT
CCTCTTGATATCGTTGAAAATCATTCCAATAACAGGAAATTGAAAGTGCTCCAGAAATTA
TCGATGAGGAAACGCCGTTCTTTCTCGAATAAAGCAGTTCTTAGGGAAAATATATTAAAC
AATTTAAATGCCTCGAATTCTACGAACAAGTTAAATGCTGGTGTTTATGTCTTTACTATC
CCCATAGTGCTTGCCAGTCGTATTCCTGAATCGCTTTACTACCCATCAGCGAGGGTATCC
TACAGCTTAAGATTAGCGACAAAATTGAAGGATGAACATACACAGTTGGTTGCATCACGG
CCACGTTCTTCTTCTATCTCTTCTCCTCAAAAACTGCGTTCATATTCCTGTTCTGATTCT
TATGAATATTCTCAAATTGATGACACCATAGAAGGAGAAACCTACAATAACGACAAAAAT
TCTACTGGAAAAATCGCATTTCCCTCCTCATGGTTAAAAAGTGCGAAGGGGCGCTTAAAA
AGGAATAATTCTAACGGAAGGTCAGATAATAATGGCGCATCTTCTAGTGGTTTAGCGATG
CAAAATGATTCTGAAGACACTATAAATTTACAGTACCCGCTTAATTTGGTGAGAACTCCG
CCAGAAATCTCTGTCACTACAGCAAATAAGCCACTTTACATTAACAAGGTTTGGGAGAAT
TGTCTTTCTTATGAAATTTCATTCGCCCAAAAGTATGTGCCCTTGAATGGTGAAATTCCA
ATCACAATTAAAGTGGCACCTCTAGTAAAAAGTCTTAGTGTCAAGAGAATTCGTGTTAGT
TGTAGAGAAAAAATCTCCTACAGGAGTAAAGACTATCAATATGATTTTGACCAGTTGGAT
CCATTGGCTTCAGATCCTTGCAATCCCTATCATATGAGATATTTAGTGAGAAAAAAGAAG
GACAGAAGCTTGCCTCTATTTGAGGTGGCCTCTAAATGTACTAGTGGGCCTTCTATTAGA
GAGGAAGTTGTCACCAATACAGTTGATGATAACTTATTAGCCTACACTTCATCAAAAGAA
AACAACAAAGATATCCCATTTTCTGAGTCCTTTACTGTTAAAACGAAGTTAAAATTTCCA
AAATATTGTGAAGTGGACGCTACCAAAGCTGCAAGTCTACCACCATATGGTATCGATCTT
TTCGACCCAATAAAAGATCCAACTCAGAGTGAGAACACCTCTAATAACGGCAATGTATTG
GGCTTTCTAGTGGGTCGCCCTAACAGGGCTTCCAAGACAGTTCATAAAATACCGCAAGAC
AAGAATCATAATGAGGTCAATGATACAAATGGAAACTCGAATACATCTTTGCAAACTAGC
TCGAATGTTCCCATTCAGCACTACACACGGTTGAATAAACCAAGACGCGGTTTATATTTG
GACAGTATGCATTTTAAGAATATCCAATGTTCTCATAAATTAGAAATCGTCTTGAGGGTT
AGCAAAACTGATAGTGGTAGCTCAAAAATCATAAGGCATTATGAAGTAATTGTTGATACA
CCAATTTATTTGATTTCTGACCTTTGTAACACTTCAAATATAGACTTACCGACTTATGAC
ATGGCCACCACTGAATCTTCTAAAGTCCTGCCACCAACTTTTGAAGAGGCAACATCAGTT
TCAGCATCACCAAGATCTTCTGTATCTTATTATCCCGATGACATTTCCATGCAACAGTTG
AATTTGTCTAGGTCGACTTCCCTCGCAAATGGTTACCTGTCAACCTTACATCCAAAAACA
ACCGCTGTTTCGGACTCGTCTAACGGTGCCCCAATCCGGGACCAGCAAGAGCAACAAGCA
CGCCCCTTGAGGACTGAAGATTATGCGCTACAAATGGGGAATGAAAATAACGCCTATAGC
AATATGGACGGTTTGCTTTCGCAGGATATCTTTGAACAGGAGACCGCGGCCACGCTATTT
AAAAGAGACATTGTCACAATGAACTTTAACAATAATATATTCACACCACGGTATAGTCCG
CGCACTTTTACCAATACCGACTACAATTATAATGATAATGATAATAATGATAATGATACA
GAGGGGCCTGGTCCAATAATTCATCCGGGTCCTGAACCACCAAGATATGACGAGATTTCA
TCATAG

Protein sequence predicted based on nucleotide sequence

MQSTVPIAIASNGNKRDVVQNVSAGDEGDILQRLARNREMISTSLSPQKSSGFSGRRRSS
SVRDALSSFFGTGNSPTSSMDDYSNLMNRNYSTASTAMCRGNSFPSDVGTKAYNITGSYQ
PDRHRNSVPYTTIDQLHTRQDTGLRRESDPVAAKQISSNNDIVRSFITHHASNSTMFINR
VLSDYLADRGFIKQTPLYNKKSVLEISIATSAESVFLPTTKSDETEYLSLIHGSLNQART
QPVGSTNTAESDFLPSCPTMDTLNENNDLSLFPLHTQRTSPSNTARTGNAMDTSNSDRAS
PASNNNTTDADSFVASGNNNPMNNNNSPARNRHPNSHSRSLPNAWNSQMPSFSFALIFSL
NKSTTLSDIKVELTSNVRVVWFNGLPPTKNVNEECYNIGSLDWTLNADNFNLFIPQGAKS
PLDIVENHSNNRKLKVLQKLSMRKRRSFSNKAVLRENILNNLNASNSTNKLNAGVYVFTI
PIVLASRIPESLYYPSARVSYSLRLATKLKDEHTQLVASRPRSSSISSPQKLRSYSCSDS
YEYSQIDDTIEGETYNNDKNSTGKIAFPSSWLKSAKGRLKRNNSNGRSDNNGASSSGLAM
QNDSEDTINLQYPLNLVRTPPEISVTTANKPLYINKVWENCLSYEISFAQKYVPLNGEIP
ITIKVAPLVKSLSVKRIRVSCREKISYRSKDYQYDFDQLDPLASDPCNPYHMRYLVRKKK
DRSLPLFEVASKCTSGPSIREEVVTNTVDDNLLAYTSSKENNKDIPFSESFTVKTKLKFP
KYCEVDATKAASLPPYGIDLFDPIKDPTQSENTSNNGNVLGFLVGRPNRASKTVHKIPQD
KNHNEVNDTNGNSNTSLQTSSNVPIQHYTRLNKPRRGLYLDSMHFKNIQCSHKLEIVLRV
SKTDSGSSKIIRHYEVIVDTPIYLISDLCNTSNIDLPTYDMATTESSKVLPPTFEEATSV
SASPRSSVSYYPDDISMQQLNLSRSTSLANGYLSTLHPKTTAVSDSSNGAPIRDQQEQQA
RPLRTEDYALQMGNENNAYSNMDGLLSQDIFEQETAATLFKRDIVTMNFNNNIFTPRYSP
RTFTNTDYNYNDNDNNDNDTEGPGPIIHPGPEPPRYDEISS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3366
Mutations:
None
Discrepancy:
c1804a,H602N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable