Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH148740.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00024285
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5313nts
Open reading frame: 1 to 5313
ATGTTCAATAGAAGTAACACCGCAGGCGGATCTCAGGCTATGAAAGAGGGACTTGGCATA
AACAAGCTCTCCCCGATATCATCGAATTCGAACCCAAGCTCATTGACTTCCTCCAATTAT
GAAAAATATCTGCAGCTGGCCACAGAGAAGAATCCGTGTATGATCTTGGAGCTGGAACTG
GACGGCAAGGTGCGATATGGCTCTCCACAGTGGAACACGATCACAGGAGTCGCCGATGAT
AGTGGCTCTTCTCCGACGTACATTGCAGACCTTATTCTCGGATCCGATCAAGATAAAGGT
GTCTTTCAAAAGGCCACAGACATGCTGCTCATGAATGATGACACCAGTTGCACTATAACG
TTCAAGATAAAGGCAGCCGACTATGAAGGTAGCGCAGGCTGTGACGATGAAAGTACGATA
ACGACCTTGGAAGCACGTGGTATCTTAATCAGGGATGGCCACACACAGTTGCCCTCTCAC
ACGATGTGGATAGTCAAGCCTCGCACAAACGACTGGTCAGACTTTTATGCCAACGAAGAC
GCTCAAGACGACATGGTCATCCAGTTATCCGATAATTGCGACGATATCGATATCCAACTT
CCCGAAGAGTTCGCCAAGACGCTTGGGTTCGGCGCTAAGATCTTCGTGCAGTACTTGAAG
AGAATACGACTGGAAATGATAATAGACGAGTTCAATCTACCTCTGCCAAAAATGGAACTA
TGCCGGGTCTGTGAGAACTTTGTCCCTGTTTGGTGGTTGGAGACCCATTCGCAAAGTTGC
GTTTGCGAGCATAGAACGGAATCGCTCATACAATTACTACACGATAATCTTCTTGAGCAA
CAGGCGATCTTGGCAAACTTCACGAAAGATTCAGAGTATAAGGGCAGTCAGATACAGGTA
CGTTCCAACAACTTCCTTAACCAAGTTTTAGACTCCTTAAGAGAGCTGTGTCAGGACGCC
ATAGATATCAACCCGAGTGAAATGGTTCCTGATCTTTACCACAGTCTTTCAACATTTCCT
CAAGATAATGGTAATAATAACAATAATAATAATAATAATAATAATAATAACAATGCTTTG
TTAGATCAATTCCCTATCCAAAAAGATACAGTTAGCTTGAATTCATATTTTCAGTTTTCC
CCAAGGACTAACCACAACATTCAAAACGTCACGTCGTGGCAATCAAGATTTTTTCTCAAT
GATGATCAGGATCCTGGACTAGCTCTTTTGATTCACGATACTCTGGACTTGGCAAGGAAA
AAAGTGGATGCCGTGTTGAGGTTGGATAACGCAATGACCTATTCTTTAAAGATTAAAAAC
GAGGTCAACAACTATGTGGTACAACTGATCCGCGAGCAAATTGAAATAAATAAGCATGCA
ATCCTAACTCACCCAATGAATTTAAGGTCTTCTTCCATATTTCATTCCCCACTGCCGCAA
ATTCACTCTCAACAACCAGAAGCCGAGAATCTCATATATTCCTCCTCTACTCCCCTGCAA
GTCCAACACGACCAATGTGCGTCCTTTGAAGCACCCTCCAAGTCTCATCTGGAGCCTATT
CCTTTCCCGGTTTCTTCCATTGAAGAAACACCAACTGCAAATGATATCAGGCATCCTTCT
CCTTTGCCCCGTAGTTGTAGCAACACCGTTATGAAACTACCGACACCTCGAAGGAAACTT
GACTCAAACGGATTATTCTCTGATGCCTATTTAAACGCTGACATCATTCCGAACCCAAGT
ATCGAATCCACGATATCTATTGATAGAGATAATAACACTAATAGTAGGGGTAGTAGTATG
AAACAGTATGGTATTGGTGAAGCCACCGACTCTCGGACTAGTAACTCGGAAAGACCTTCT
TCCTCTTCGTCAAGGCTGGGGATAAGATCAAGATCCATAACACCAAGACAAAAGATAGAA
TACTCACATGTAGATAATGATGACCGCACCAACGAAATGCTGTCTAGAGATAAAGATTCT
CTTCAACCTCAACCTTCCGTAGATACCACCATAACATCCTCTACTCAGGCGACCACCACG
GGTACCAAGACTAATAGTAACAATTCCACAAACTCAGTATTACCAAAACTAATGACAAGT
ATTTCCTTGACCCCAAGGCGTGGTTCACCATCATTTGGTAATCTCGCAAGCCATTCTATG
CAGCAGACAAACAGTTTTAAACTGATTCATGATAAATCGCCGATATCTTCACCTTTCACA
TTCTCCAAGGATTTTTTAACCCCAGAGCAGCACCCTTCCAATATTGCCAGAACAGATAGT
ATCAATAATGCAATGTTAACTTCACCGAATATGCCATTATCACCCCTTTTATTGGCCACA
AACCAAACTGTTAAATCTCCAACGCCTAGCATAAAAGATTACGATATCTTGAAACCAATC
AGCAAAGGTGCTTATGGTAGTGTTTATCTAGCACGGAAAAAACTCACAGGAGATTATTTT
GCTATAAAGGTTCTAAGGAAATCAGATATGATTGCCAAAAATCAAGTAACAAATGTCAAA
TCCGAGAGAGCAATCATGATGGTTCAAAGTGATAAGCCCTATGTTGCGAGACTATTTGCT
AGTTTCCAAAATAAAGATAACCTTTTCTTAGTGATGGAATATTTACCAGGTGGAGATTTG
GCCACTTTAATCAAGATGATGGGGTATCTGCCCGATCAATGGGCCAAGCAATACCTAACC
GAAATCGTTGTCGGTGTGAATGATATGCATCAAAATGGGATCATTCATCATGACTTAAAG
CCTGAAAATCTACTAATTGATAATGCAGGTCATGTGAAATTAACAGATTTCGGTTTATCA
AGAGCTGGTCTGATTCGCCGTCACAAGTTTGTCCCACATAAGTCGTCGCTAAGTATCAGT
TCCACTTTACCAATCGATAACCCAGCAAATAATTTTACCATGAACAACAACAATAGTAAT
CATTCTCAATTATCAACCCCAGATAGCTTCACATCAGATCATAAGCAGTATAATAGAAGC
AAGAAGTCATCACTAGGTCAGCAATACGAACACTCAGAATACTCAAGTACTTCCAATTCC
CACTCAATGACGCCAACGCCCAGTACGAACACTGTTGTTTATCCTTCATATTACCGTGGG
AAGGACAGATCACACGGAAGTTCGAACATCGATCTCCCAGCGTCCCTTAGAAGAAGTGAA
TCTCAATTATCATTTTCCCTCCTTGATATTTCTCGTTCTAGTACTCCTCCTTTAGCAAAT
CCCACAAATTCGAACGCTAATAATATTATGAGAAGGAAATCACTCACTGAGAATAAATCC
TTTTCTAATGACCTATTATCTTCAGATGCTATCGCAGCTACCAATACGAATATTAACTCG
AATAATAACATTTCCCTTTCGCCAGCACCTTCGGATTTAGCTTTGTTTTATCCTGATGAT
AGCAAGCAAAATAAGAAATTTTTTGGGACTCCCGATTATCTCGCTCCAGAAACTATTGAA
GGAAAGGGTGAAGATAACAAGCAATGCGACTGGTGGTCAGTTGGTTGTATATTTTTCGAA
TTACTTTTAGGGTATCCTCCATTCCATGCAGAAACACCAGATGCTGTTTTTAAGAAAATT
CTATCAGGAGTCATTCAATGGCCAGAGTTTAAAAATGAAGAAGAAGAGCGAGAATTCCTA
ACACCAGAGGCAAAAGATTTGATAGAAAAATTGTTGGTTGTGGATCCTGCGAAAAGACTG
GGTGCGAAAGGAATTCAAGAAATTAAAGATCACCCTTATTTCAAGAATGTGGATTGGGAT
CATGTTTACGATGAGGAAGCTTCTTTTGTCCCTACAATAGACAATCCAGAAGATACTGAT
TATTTTGACCTAAGGGGTGCAGAGCTCCAAGATTTTGGAGACGATATCGAAAACGATAAT
GCCAATATTTTGTTTGGTAAACATGGCATTAACACCGATGTTTCTGAATTATCTGCAGCT
AATCTCTCTCCACCATTGAATCATAAAAATATTTTATCCCGTAAACTATCGATGAGTAAC
ACCACTAATAGGAGCTCAAATAATTCCAACAGTAGCGTGCATGACTTTGGTGCACATACA
CCGGTTAATAAATTAAGTATTGCTTCTGTATTAGAGTCAGTACCTCAAGAAACAGGATAT
ATTACACCTAACGGGACCGGTACAACTACTACAAGTGCCAAAAACTCACCCAATCTGAAG
AATTTGTCACTGGCTATACCTCCACATATGAGGGATCGCAGATCAAGTAAATTGAATGAT
TCACAAACGGAATTTGGTTCTTTTAATTTCAGGAATTTATCGGCTCTTGATAAAGCTAAT
AAAGATGCTATAAATAGACTGAAAAGTGAACATTTTTCTGAACAACCTGGGGTTCACAGA
AGAACCTCTTCTGCGTCACTAATGGGGTCATCCTCAGACGGATCAGTGTCAACTCCAGGG
AGTAACGCTTCAAACACTACATCTGGTGGCAAGTTGAAAATACATAAGCCTACCATATCC
GGTTCTCCTTCAACATTTGGCACATTTCCCAAAACATTTTTGAGGTCTGATTCATTCTCC
ACAAGATCATATTCTCCTGAACGAAGTATTAGTATCGACTCGTCAACATTATCAAGGAAG
GGTAGTATAATCGGGGATAACCAACAAACAACAGCAAATAGCTCGGATTCACCTACGATG
ACTAAATTCAAGTCGCCACTATCACCTGCTAATACCACCACCGTGAGCTCATATTTTTCA
AGACAGAGGGTTCTATCAAAGAGTTTTTCGCAACGGACCAATTCCAGTGATCTCTCGGCA
GAGGAAAGCGACCGACTACAGGCTATATCAAGAGTTAACTCTTTAAGAAACAGGAGGCGT
AGTGGCCGAAAGAGCTCGAGCACTTCTGAGATTGGATACCACATGGATGTTCTTGTTTGT
GAGCCTATACCGATTCATAGATATCGGGTTACTAAAGACTTAGAAAATTTGGGCTGTACC
GTCGTCAGTGTTGGTGCCGGTGATGAACTAGTTAGTAGAGCCACTAGTGGTGTAAGTTTT
GACTTAATTATGACAGCCTTGAAGCTTCCAAAACTTGGTGCTATTGACATTGTTCAACTA
CTAAAGCAAACAAATGGTGCTAATTCGACAACACCAATTGTGGCCATAACAAATTATTTT
CAGGAGGCGGCAACCAGTAGAGTCTTTGACGATGTTTTAGAAAAACCGGTAAAACTTGAC
GAGCTAAAAAAATTGGTGGCTAAGTACGCACTGAAAAAGTCTCAAGAAGATGAAGAGCAT
ACTATATTGAGCGATTCTGATGAAACGCACTAG
Protein sequence predicted based on nucleotide sequence
MFNRSNTAGGSQAMKEGLGINKLSPISSNSNPSSLTSSNYEKYLQLATEKNPCMILELEL DGKVRYGSPQWNTITGVADDSGSSPTYIADLILGSDQDKGVFQKATDMLLMNDDTSCTIT FKIKAADYEGSAGCDDESTITTLEARGILIRDGHTQLPSHTMWIVKPRTNDWSDFYANED AQDDMVIQLSDNCDDIDIQLPEEFAKTLGFGAKIFVQYLKRIRLEMIIDEFNLPLPKMEL CRVCENFVPVWWLETHSQSCVCEHRTESLIQLLHDNLLEQQAILANFTKDSEYKGSQIQV RSNNFLNQVLDSLRELCQDAIDINPSEMVPDLYHSLSTFPQDNGNNNNNNNNNNNNNNAL LDQFPIQKDTVSLNSYFQFSPRTNHNIQNVTSWQSRFFLNDDQDPGLALLIHDTLDLARK KVDAVLRLDNAMTYSLKIKNEVNNYVVQLIREQIEINKHAILTHPMNLRSSSIFHSPLPQ IHSQQPEAENLIYSSSTPLQVQHDQCASFEAPSKSHLEPIPFPVSSIEETPTANDIRHPS PLPRSCSNTVMKLPTPRRKLDSNGLFSDAYLNADIIPNPSIESTISIDRDNNTNSRGSSM KQYGIGEATDSRTSNSERPSSSSSRLGIRSRSITPRQKIEYSHVDNDDRTNEMLSRDKDS LQPQPSVDTTITSSTQATTTGTKTNSNNSTNSVLPKLMTSISLTPRRGSPSFGNLASHSM QQTNSFKLIHDKSPISSPFTFSKDFLTPEQHPSNIARTDSINNAMLTSPNMPLSPLLLAT NQTVKSPTPSIKDYDILKPISKGAYGSVYLARKKLTGDYFAIKVLRKSDMIAKNQVTNVK SERAIMMVQSDKPYVARLFASFQNKDNLFLVMEYLPGGDLATLIKMMGYLPDQWAKQYLT EIVVGVNDMHQNGIIHHDLKPENLLIDNAGHVKLTDFGLSRAGLIRRHKFVPHKSSLSIS STLPIDNPANNFTMNNNNSNHSQLSTPDSFTSDHKQYNRSKKSSLGQQYEHSEYSSTSNS HSMTPTPSTNTVVYPSYYRGKDRSHGSSNIDLPASLRRSESQLSFSLLDISRSSTPPLAN PTNSNANNIMRRKSLTENKSFSNDLLSSDAIAATNTNINSNNNISLSPAPSDLALFYPDD SKQNKKFFGTPDYLAPETIEGKGEDNKQCDWWSVGCIFFELLLGYPPFHAETPDAVFKKI LSGVIQWPEFKNEEEEREFLTPEAKDLIEKLLVVDPAKRLGAKGIQEIKDHPYFKNVDWD HVYDEEASFVPTIDNPEDTDYFDLRGAELQDFGDDIENDNANILFGKHGINTDVSELSAA NLSPPLNHKNILSRKLSMSNTTNRSSNNSNSSVHDFGAHTPVNKLSIASVLESVPQETGY ITPNGTGTTTTSAKNSPNLKNLSLAIPPHMRDRRSSKLNDSQTEFGSFNFRNLSALDKAN KDAINRLKSEHFSEQPGVHRRTSSASLMGSSSDGSVSTPGSNASNTTSGGKLKIHKPTIS GSPSTFGTFPKTFLRSDSFSTRSYSPERSISIDSSTLSRKGSIIGDNQQTTANSSDSPTM TKFKSPLSPANTTTVSSYFSRQRVLSKSFSQRTNSSDLSAEESDRLQAISRVNSLRNRRR SGRKSSSTSEIGYHMDVLVCEPIPIHRYRVTKDLENLGCTVVSVGAGDELVSRATSGVSF DLIMTALKLPKLGAIDIVQLLKQTNGANSTTPIVAITNYFQEAATSRVFDDVLEKPVKLD ELKKLVAKYALKKSQEDEEHTILSDSDETH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
5313
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
