GEA2 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148823.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00024287
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4380nts
Open reading frame: 1 to 4380

ATGAGTGATAGGGAATTCGTCACGGTAGATCCCGTCACTATCATAATCAAAGAATGCATT
AATTTATCGACAGCGATGCGGAAATACTCTAAATTTACCTCTCAATCTGGAGTGGCCGCT
TTGCTGGGGGGAGGAAGTGAAATATTTAGCAATCAAGATGACTACTTGGCTCACACATTC
AACAATTTGAATACCAACAAGCACAATGATCCATTTTTATCTGGATTCATTCAGTTAAGA
CTTATGTTGAATAAACTGAAAAATCTAGATAATATAGATTCACTAACCATATTGCAGCCA
TTTTTATTAATTGTGAGTACAAGTTCCATTTCTGGTTACATCACTTCCCTGGCCCTGGAC
TCTTTGCAGAAATTCTTTACCTTGAATATCATCAATGAATCATCGCAAAACTATATTGGT
GCACACAGGGCGACGGTAAATGCTCTAACACATTGTAGGTTTGAAGGATCTCAACAACTT
TCTGATGATTCAGTTCTTTTGAAAGTCGTGTTTTTACTGCGTTCAATCGTCGACTCACCT
TACGGAGATTTATTATCAAACTCTATCATATATGACGTATTGCAAACGATTCTTTCATTG
GCTTGTAATAACAGAAGGAGCGAAGTCCTTAGGAATGCTGCACAATCAACAATGATAGCC
GTTACCGTAAAGATTTTCTCAAAACTAAAGACTATTGAGCCTGTTAATGTGAATCAAATA
TACATCAATGATGAAAGTTACACAAATGATGTATTGAAGGCCGATACAATTGGCACAAAT
GTAGAATCCAAAGAAGAAGGAAGTCAAGAAGATCCCATCGGCATGAAAGTGAATAATGAG
GAAGCTATTAGCGAGGACGATGGCATTGAAGAAGAGCATATTCATTCAGAGAAGAGCACA
AATGGCGCCGAACAACTAGATATTGTGCAAAAAACAACAAGATCAAATTCCAGGATCCAA
GCGTATGCTGATGATAACTATGGATTGCCCGTGGTTAGGCAATATTTAAACTTATTACTA
TCATTGATTGCGCCAGAAAATGAATTAAAACATTCATACTCCACTAGAATATTTGGCCTA
GAGTTAATTCAAACGGCATTAGAAATTTCAGGTGATCGATTGCAGCTATACCCACGGCTT
TTTACACTGATATCAGATCCTATTTTCAAAAGCATTTTGTTTATCATACAGAACACTACA
AAATTATCACTACTTCAAGCTACATTGCAGCTATTTACTACTCTAGTTGTTATATTGGGC
AACAACTTACAATTACAGATCGAGCTCACTCTAACAAGAATATTTTCTATTCTTTTAGAT
GATGGTACCGCAAATAACTCGAGTTCTGAAAATAAGAACAAGCCATCAATAATAAAGGAA
CTTCTAATTGAGCAAATATCCATCTTATGGACTAGGTCGCCATCTTTTTTTACTTCTACT
TTTATCAATTTCGATTGTAATCTCGATAGGGCAGACGTTTCCATAAACTTTTTGAAGGCT
TTGACTAAATTGGCCTTACCAGAATCCGCCTTAACTACCACAGAAAGTGTACCACCCATT
TGCCTTGAGGGATTGGTCTCCCTAGTCGATGATATGTTCGATCACATGAAGGACATTGAC
AGAGAAGAATTTGGCAGGCAAAAGAATGAAATGGAAATCTTAAAAAAGAGGGACCGTAAA
ACAGAGTTTATTGAATGTACCAATGCATTCAATGAAAAGCCCAAAAAGGGTATTCCGATG
TTAATAGAAAAAGGTTTCATTGCTTCCGACTCCGATAAAGATATTGCGGAGTTTCTTTTC
AATAATAACAACCGTATGAATAAAAAAACAATCGGTTTGCTACTTTGCCATCCGGACAAA
GTAAGCTTGTTGAATGAATATATTCGTTTGTTTGATTTTTCAGGGTTAAGGGTCGATGAA
GCTATTAGAATTTTGTTGACGAAATTTAGGTTGCCTGGTGAATCGCAACAAATTGAAAGA
ATCATCGAAGCCTTCTCGTCTGCGTATTGTGAAAATCAAGATTACGATCCATCCAAAATC
AGTGACAACGCGGAGGATGACATTTCTACTGTTCAACCAGACGCTGATTCTGTTTTCATT
TTAAGTTATTCAATTATTATGTTGAACACTGACCTACATAACCCTCAAGTGAAGGAACAC
ATGTCATTTGAAGATTACTCTGGTAACTTAAAGGGATGCTGTAATCACAAAGACTTCCCA
TTCTGGTATTTGGATAGAATTTACTGTTCAATCAGAGATAAAGAAATTGTTATGCCTGAA
GAGCACCACGGCAACGAAAAGTGGTTTGAAGATGCTTGGAATAACTTGATATCTTCAACT
ACTGTTATAACTGAAATAAAAAAAGACACACAATCTGTCATGGATAAATTAACACCCTTG
GAGCTTTTGAACTTTGATAGAGCAATTTTTAAACAAGTTGGCCCAAGTATTGTCAGTACT
TTATTCAACATTTACGTAGTTGCATCTGATGACCATATATCTACCAGAATGATAACAAGT
TTGGACAAATGTTCCTATATTTCCGCATTTTTTGACTTCAAAGATCTCTTTAATGATATA
CTAAACTCCATTGCTAAGGGCACTACTTTGATTAATTCAAGCCATGACGATGAACTTTCA
ACTTTAGCTTTTGAATATGGCCCAATGCCACTGGTGCAAATTAAATTCGAAGACACTAAC
ACTGAGATCCCGGTTAGTACAGATGCTGTTAGATTTGGTAGATCATTTAAGGGTCAACTA
AATACAGTTGTTTTTTTCCGGATTATTCGCAGGAACAAAGATCCTAAAATTTTCTCCAAG
GAATTATGGTTAAACATTGTTAATATTATACTAACATTGTACGAAGACTTGATTTTGTCT
CCTGATATTTTCCCTGATTTACAAAAAAGACTGAAATTAAGCAACTTGCCTAAGCCATCT
CCTGAAATTTCTATTAACAAGAGCAAAGAAAGCAAAGGTCTCTTATCAACATTTGCTTCT
TATTTAAAAGGTGATGAAGAACCCACAGAAGAGGAAATCAAATCCTCAAAAAAAGCGATG
GAGTGCATAAAGTCGAGTAATATTGCCGCCTCTGTCTTTGGAAATGAATCAAATATAACA
GCGGATTTAATAAAAACTTTACTAGACTCCGCCAAAACTGAGAAAAACGCAGATAATTCC
AGGTATTTTGAAGCAGAACTTTTATTTATCATCGAATTGACTATTGCATTATTTCTATTT
TGCAAAGAGGAGAAAGAATTAGGAAAGTTCATACTTCAAAAAGTTTTCCAACTTTCTCAC
ACGAAAGGCCTCACGAAAAGGACTGTTCGTAGAATGCTAACATACAAAATTTTGTTAATT
TCGTTATGTGCGGATCAGACGGAGTACTTGTCCAAATTAATAAACGATGAGCTGTTAAAA
AAGGGGGATATTTTTACCCAAAAATTTTTTGCAACTAATCAAGGTAAGGAATTTTTGAAG
AGACTATTTTCATTGACCGAATCAGAGTTTTATAGAGGATTTTTACTAGGAAATGAGAAT
TTTTGGAAATTTTTAAGAAAAGTTACAGCAATGAAAGAGCAGAGCGAGAGCATTTTTGAA
TATTTAAATGAATCGATCAAGACAGACAGCAATATTTTGACAAATGAGAACTTCATGTGG
GTCCTAGGACTATTAGATGAAATTTCATCAATGGGTGCCGTTGGAAATCACTGGGAAATA
GAATACAAGAAATTGACAGAAAGTGGTCATAAAATTGATAAGGAGAATCCATACAAGAAA
TCGATCGAATTATCATTGAAATCCATTCAACTAACATCACACTTGCTGGAAGATAATAAC
GATCTGCGTAAAAACGAGATATTCGCTATTATTCAAGCTTTGGCACATCAATGCATCAAT
CCGTGTAAGCAGATAAGTGAATTTGCAGTGGTAACGCTAGAGCAGACGCTCATCAATAAA
ATCGAAATTCCAACTAATGAGATGGAATCGGTAGAAGAATTAATTGAGGGCGGATTACTA
CCGTTGCTAAATTCGAGTGAAACACAGGAAGACCAGAAAATCCTCATTTCATCCATATTA
ACAATAATTTCAAATGTTTATTTGCATTATTTGAAACTAGGGAAGACAAGCAACGAAACG
TTTTTGAAAATTTTGAGTATTTTCAATAAATTTGTAGAGGACTCAGATATTGAAAAAAAG
CTACAGCAATTAATACTTGATAAGAAGAGTATTGAGAAGGGCAACGGTTCATCATCTCAT
GGATCTGCACATGAACAAACACCAGAGTCAAACGACGTTGAAATTGAGGCTACTGCGCCA
ATTGATGACAATACAGACGATGATAACAAACCGAAGTTATCTGATGTAGAAAAGGATTAG

Protein sequence predicted based on nucleotide sequence

MSDREFVTVDPVTIIIKECINLSTAMRKYSKFTSQSGVAALLGGGSEIFSNQDDYLAHTF
NNLNTNKHNDPFLSGFIQLRLMLNKLKNLDNIDSLTILQPFLLIVSTSSISGYITSLALD
SLQKFFTLNIINESSQNYIGAHRATVNALTHCRFEGSQQLSDDSVLLKVVFLLRSIVDSP
YGDLLSNSIIYDVLQTILSLACNNRRSEVLRNAAQSTMIAVTVKIFSKLKTIEPVNVNQI
YINDESYTNDVLKADTIGTNVESKEEGSQEDPIGMKVNNEEAISEDDGIEEEHIHSEKST
NGAEQLDIVQKTTRSNSRIQAYADDNYGLPVVRQYLNLLLSLIAPENELKHSYSTRIFGL
ELIQTALEISGDRLQLYPRLFTLISDPIFKSILFIIQNTTKLSLLQATLQLFTTLVVILG
NNLQLQIELTLTRIFSILLDDGTANNSSSENKNKPSIIKELLIEQISILWTRSPSFFTST
FINFDCNLDRADVSINFLKALTKLALPESALTTTESVPPICLEGLVSLVDDMFDHMKDID
REEFGRQKNEMEILKKRDRKTEFIECTNAFNEKPKKGIPMLIEKGFIASDSDKDIAEFLF
NNNNRMNKKTIGLLLCHPDKVSLLNEYIRLFDFSGLRVDEAIRILLTKFRLPGESQQIER
IIEAFSSAYCENQDYDPSKISDNAEDDISTVQPDADSVFILSYSIIMLNTDLHNPQVKEH
MSFEDYSGNLKGCCNHKDFPFWYLDRIYCSIRDKEIVMPEEHHGNEKWFEDAWNNLISST
TVITEIKKDTQSVMDKLTPLELLNFDRAIFKQVGPSIVSTLFNIYVVASDDHISTRMITS
LDKCSYISAFFDFKDLFNDILNSIAKGTTLINSSHDDELSTLAFEYGPMPLVQIKFEDTN
TEIPVSTDAVRFGRSFKGQLNTVVFFRIIRRNKDPKIFSKELWLNIVNIILTLYEDLILS
PDIFPDLQKRLKLSNLPKPSPEISINKSKESKGLLSTFASYLKGDEEPTEEEIKSSKKAM
ECIKSSNIAASVFGNESNITADLIKTLLDSAKTEKNADNSRYFEAELLFIIELTIALFLF
CKEEKELGKFILQKVFQLSHTKGLTKRTVRRMLTYKILLISLCADQTEYLSKLINDELLK
KGDIFTQKFFATNQGKEFLKRLFSLTESEFYRGFLLGNENFWKFLRKVTAMKEQSESIFE
YLNESIKTDSNILTNENFMWVLGLLDEISSMGAVGNHWEIEYKKLTESGHKIDKENPYKK
SIELSLKSIQLTSHLLEDNNDLRKNEIFAIIQALAHQCINPCKQISEFAVVTLEQTLINK
IEIPTNEMESVEELIEGGLLPLLNSSETQEDQKILISSILTIISNVYLHYLKLGKTSNET
FLKILSIFNKFVEDSDIEKKLQQLILDKKSIEKGNGSSSHGSAHEQTPESNDVEIEATAP
IDDNTDDDNKPKLSDVEKD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4380
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable