Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH148828.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00024288
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5904nts
Open reading frame: 1 to 5904
ATGTCAACTGAATACTCTGCTGATTCATCTAAATCATTTATGATTGCAATGCAATCTATG
ATTGATACTTCACAAACCTTTAATCTAGATAGATCGAAAATCTCTTTACCAGATTTTGAT
GACGAGCTAAAAAAAGTCCAAAAGGATGAACAAAATCAAAGAACTGAACTAACTGTTCTC
TCACAAGATAGAAATGATTGGGATGATATATTTGAGGAGTTTAAAGACATTTCATTTGCT
CAACTGCAATCTATAATTGATTCATACAAAACAAAGAATGCAGTTGCAGTATATAAAAAA
ATTGGAAAACTAATCAATGAAGCTGAAACGACACTAAGCAGCAATGTATTATTAGAAACT
GTACTGCAAATGGTTTATAAACATCAAAAGCAAGAGTTAGAGAAAGAATTGTTGGATTTT
TTAGGAACAGGGAATATAGACCTTGTTTCTCTGTTACTTCAGCATAGAAGAATGATCGTT
GCTACACCTATAGAAACGACGATTCTGCTAATAAAAAATGCGGTCAATTCAACGCCAGAG
TTCCTGACACAGCAAGATATCAGGAATCAAGTTTTGAAAAGTGCAGAGGATGCCAAGAAT
AGGAAATTAAATCCCGCCACTAAAATAATCAAGTACCCACACGTCTTCAGAAAATATGAA
GCAGGATCCACTACAGCTATGGCCTTTGCCGGTCAAAAATTCACTTTGCCAGTAGGGACT
ACAAGGATGTCGTACAATACCCATGAAGAAATTATAATTCCGGCAGCAGATCAGGCATCC
AACAAGAACTATCTCTATACAAAACTATTGAAAATAAGTGATTTGGATCATTTTTGCAAG
ACTGTATTTCCCTATGAAACTTTAAACCAAATACAATCCCTTGTTTACCCCGTAGCTTAT
AAAACAAATGAAAATATGCTAATTTGTGCACCTACTGGTGCAGGTAAAACAGACATTGCA
TTACTGACAATAATTAATACAATAAAACAATTCTCCGTGGTCAACGGGGAAAATGAAATT
GATATTCAATATGATGATTTTAAAGTTATTTATGTTGCCCCTTTGAAAGCACTCGCTGCT
GAAATCGTCGACAAGTTTAGTAAGAAATTGGCGCCTTTTAATATACAAGTAAGGGAGTTA
ACAGGCGATATGCAATTGACAAAGGCGGAAATTTTAGCCACTCAAGTAATAGTCACAACC
CCTGAGAAATGGGACGTTGTCACCCGTAAAGCAAACGGCGACAACGATTTGGTTTCAAAG
GTCAAATTACTAATTATTGATGAAGTTCATTTACTGCACGAAGATAGAGGTTCGGTTATT
GAAACTTTAGTTGCACGTACCTTGAGACAGGTAGAAAGCTCTCAATCAATGATTCGTATT
ATCGGTCTATCAGCTACATTACCCAATTTTATGGATGTAGCAGATTTCTTAGGAGTAAAT
AGACAGATCGGAATGTTCTATTTTGATCAATCATTCCGTCCAAAGCCTCTAGAGCAGCAA
CTTTTGGGCTGTAGAGGTAAGGCAGGTAGTAGACAAAGTAAAGAGAATATCGATAAAGTC
GCTTACGACAAATTGAGTGAAATGATTCAAAGAGGGTACCAGGTAATGGTATTTGTCCAC
TCAAGAAAGGAAACTGTTAAAAGTGCAAGAAACTTTATCAAACTAGCAGAATCTAACCAC
GAAGTTGACCTTTTTGCACCTGACCCTATCGAAAAGGATAAATATTCTAGAAGTTTAGTG
AAGAACAGAGACAAGGATATGAAGGAGATTTTTCAATTTGGTTTTGGTATCCATCATGCT
GGTATGGCCCGTTCAGATAGAAATCTTACTGAAAAAATGTTTAAAGACGGTGCTATCAAG
GTACTGTGTTGTACTGCAACATTAGCATGGGGTGTTAATTTACCTGCTGATTGTGTTATC
ATCAAGGGTACACAAGTATATGATTCTAAAAAGGGTGGATTCATAGATCTAGGTATTTCA
GATGTTATTCAGATTTTTGGCAGGGGTGGTAGGCCGGGCTTCGGTTCCGCAAATGGTACG
GGTATTTTATGTACTTCCAATGATCGTCTGGATCATTATGTTTCCTTAATTACTCAACAA
CACCCTATCGAATCTAGATTCGGTTCCAAGTTGGTTGATAACCTGAACGCCGAAATTTCA
TTGGGGAGCGTGACAAATGTAGATGAAGCAATCGAATGGCTTGGTTACACATACATGTTT
GTTCGTATGAGAAAAAATCCTTTTACTTACGGTATTGATTGGGAGGAAATTGCAAATGAC
CCGCAATTATATGAACGGAGGAGAAAAATGATCGTAGTAGCTGCAAGGCGATTACATGCT
CTCCAAATGATTGTGTTCGATGAAGTGTCAATGCATTTCATTGCAAAGGATTTGGGCAGG
GTGTCCTCTGATTTTTATCTTTTGAACGAATCCGTCGAGATATTCAATCAAATGTGTGAT
CCCAGAGCCACCGAAGCTGATGTATTGTCCATGATCAGTATGAGCAGTGAATTTGACGGT
ATAAAATTTAGAGAAGAAGAATCCAAGGAGTTGAAGAGACTTTCTGATGAATCTGTTGAG
TGCCAGATTGGAAGCCAACTTGACACCCCTCAAGGTAAAGCAAACGTGCTATTACAGGCT
TATATTTCCCAGACTAGAATTTTTGACTCAGCACTATCATCCGATTCGAATTATGTTGCT
CAGAACTCTGTTAGAATTTGTAGAGCATTGTTTTTAATAGGTGTCAATAGAAGATGGGGT
AAATTTTCAAACGTCATGCTAAATATTTGCAAATCTATTGAAAAGAGGCTCTGGGCCTTT
GATCACCCTCTCTGTCAGTTTGATTTGCCTGAAAATATTATCAGACGCATCAGAGATACT
AAACCTTCCATGGAGCATCTTTTAGAATTAGAGGCAGATGAGCTCGGAGAGCTGGTGCAT
AATAAGAAGGCCGGGTCTAGGTTATATAAAATATTGAGCCGATTTCCAAAGATTAATATA
GAAGCTGAGATTTTCCCCATTACAACAAATGTAATGAGGATACATATTGCATTAGGTCCA
GACTTTGTATGGGATTCCCGGATACATGGTGATGCACAGTTTTTTTGGGTTTTCGTTGAA
GAATCTGATAAGTCGCAAATCCTTCATTTTGAAAAGTTCATCTTAAACAGAAGGCAGTTG
AATAATCAGCATGAAATGGACTTCATGATCCCATTGTCAGACCCTTTGCCTCCGCAGGTT
GTCGTAAAGGTTGTGTCGGATACTTGGATTGGCTGTGAATCGACCCATGCCATCTCTTTT
CAACATTTAATCAGACCATTTAACGAAACTTTACAGACAAAATTGTTGAAGTTACGTCCT
CTACCTACATCAGCCCTCCAGAATCCTTTAATTGAGTCAATTTATCCTTTCAAGTATTTC
AATCCCATGCAAACTATGACATTTTATACACTTTACAATACCAATGAGAATGCGTTTGTT
GGCTCGCCTACAGGTTCTGGTAAAACGATTGTGGCTGAATTGGCTATATGGCATGCCTTC
AAAACATTTCCTGGGAAAAAGATTGTTTATATCGCACCAATGAAAGCATTAGTACGTGAA
AGGGTTGATGACTGGAGAAAGAAAATAACACCAGTGACCGGAGATAAAGTTGTAGAGTTA
ACCGGTGATTCTTTGCCAGATCCTAAAGATGTGCATGATGCCACCATTGTTATTACAACT
CCAGAAAAGTTTGATGGTATTTCCCGTAATTGGCAAACTCGTAAGTTTGTTCAAGATGTA
TCTTTAATTATCATGGATGAAATTCATTTGTTGGCTAGTGATCGTGGTCCTATCTTAGAA
ATGATTGTTAGTCGTATGAATTATATTTCCTCTCAAACCAAGCAACCGGTCAGACTGCTA
GGTATGTCAACTGCTGTTTCAAACGCCTACGACATGGCTGGCTGGTTGGGAGTTAAGGAC
CACGGACTGTATAACTTCCCGTCAAGCGTCCGTCCGGTTCCATTGAAAATGTACATTGAT
GGATTTCCAGATAATTTGGCATTCTGTCCTCTGATGAAAACAATGAACAAACCCGTGTTT
ATGGCCATTAAACAACATTCACCTGATAAACCAGCATTAATATTTGTTGCATCGCGTAGA
CAAACTAGACTAACGGCTTTAGACTTGATCCACTTATGTGGAATGGAAGATAATCCACGT
AGGTTTTTGAATATAGACGATGAGGAGGAACTGCAATATTACTTATCACAGGTCACTGAC
GACACTTTAAAGTTATCACTCCAGTTCGGTATTGGATTGCATCATGCAGGTCTAGTGCAA
AAAGATCGTTCTATTTCTCATCAGTTGTTTCAAAAAAACAAAATTCAGATTCTGATCGCT
ACATCAACCCTAGCGTGGGGTGTCAATTTGCCAGCTCACTTGGTTATCATCAAAGGTACA
CAATTTTTTGATGCAAAAATTGAAGGATATAGAGATATGGATTTAACAGATATCTTGCAA
ATGATGGGGAGAGCTGGTAGACCGGCTTATGACACTACAGGTACTGCTATCGTTTATACA
AAAGAGTCCAAAAAAATGTTTTATAAACATTTCCTAAACGTTGGTTTTCCTGTCGAGTCC
TCCTTGCACAAGGTATTAGATGATCATTTAGGTGCAGAAATAACGTCAGGTAGCATAACT
AATAAGCAAGAAGCGTTAGATTTTTTGAGTTGGACATTTTTGTTTCGTAGAGCGCATCAT
AACCCGACATATTATGGCATTGAAGATGATACAAGTACAGCAGGAGTCAGTGAACATTTG
AGTTCTTTGATAGATAGCACATTAGAAAATCTGCGAGAATCACAATGTGTTTTATTACAT
GGAGATGATATTGTTGCTACGCCATTCTTAAGTATATCCTCATACTACTATATTTCACAT
TTAACGATACGCCAATTATTGAAACAGATACATGATCATGCGACTTTTCAAGAGGTATTA
CGATGGTTATCGTTGGCTGTTGAGTATAATGAATTGCCTGTAAGAGGTGGTGAAATTATC
ATGAATGAAGAAATGTCGCAGCAATCGAGGTACTCCGTTGAAAGCACTTTTACCGATGAA
TTCGAATTACCGATGTGGGATCCACATGTAAAAACATTTTTATTACTTCAAGCTCATTTA
AGCCGTGTAGATTTACCGATTGCAGATTATATCCAGGATACCGTATCAGTTTTGGATCAA
TCTCTACGTATTCTACAGGCTTATATTGATGTCGCCAGTGAATTGGGTTACTTTCATACG
GTATTGACCATGATAAAGATGATGCAATGCATAAAGCAAGGGTATTGGTATGAAGACGAT
CCTGTTAGTGTACTTCCAGGATTACAACTGAGAAGAATCAAAGATTACACTTTTAGCGAG
CAGGGGTTCATTGAAATGACGCCGCAGCAAAAGAAGAAAAAGCTGTTAACCTTGGAGGAA
ATAGGAAGGTTTGGATATAAGAAATTATTAAATGTTTTTGATCAACTAACTTTTGGAATG
ACAGAAAGCGAAGATACGAAAAAACGTTTCGTCAGTGTCTGCCAAAGATTACCGGTTTTA
GAAGGCATGAAATTTGAAGAACAGGAAAATAACGAGGTGTTAACATTTTATAGCAAGCAC
CTTTCTAGTAAGCATAATAATGGGTTTGAAGTATATTGTGATAAATTCCCTAAGATACAA
AAAGAACTATGGTTTTTAATTGGACACAAAGGCGATGAACTGCTGATGATTAAAAGGTGT
CAACCAAAACAAATGAATAAAGAGGTCATAATACATTGTGATCTATTTATTCCCGAAGAA
ATTCGTGGCGAAGAATTGCAATTTTCCCTTATTAATGACGCTTTAGGATTACGTTATGAT
ATGGTCCATAAACTAATCTCTTAG
Protein sequence predicted based on nucleotide sequence
MSTEYSADSSKSFMIAMQSMIDTSQTFNLDRSKISLPDFDDELKKVQKDEQNQRTELTVL SQDRNDWDDIFEEFKDISFAQLQSIIDSYKTKNAVAVYKKIGKLINEAETTLSSNVLLET VLQMVYKHQKQELEKELLDFLGTGNIDLVSLLLQHRRMIVATPIETTILLIKNAVNSTPE FLTQQDIRNQVLKSAEDAKNRKLNPATKIIKYPHVFRKYEAGSTTAMAFAGQKFTLPVGT TRMSYNTHEEIIIPAADQASNKNYLYTKLLKISDLDHFCKTVFPYETLNQIQSLVYPVAY KTNENMLICAPTGAGKTDIALLTIINTIKQFSVVNGENEIDIQYDDFKVIYVAPLKALAA EIVDKFSKKLAPFNIQVRELTGDMQLTKAEILATQVIVTTPEKWDVVTRKANGDNDLVSK VKLLIIDEVHLLHEDRGSVIETLVARTLRQVESSQSMIRIIGLSATLPNFMDVADFLGVN RQIGMFYFDQSFRPKPLEQQLLGCRGKAGSRQSKENIDKVAYDKLSEMIQRGYQVMVFVH SRKETVKSARNFIKLAESNHEVDLFAPDPIEKDKYSRSLVKNRDKDMKEIFQFGFGIHHA GMARSDRNLTEKMFKDGAIKVLCCTATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGIS DVIQIFGRGGRPGFGSANGTGILCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEIS LGSVTNVDEAIEWLGYTYMFVRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAARRLHA LQMIVFDEVSMHFIAKDLGRVSSDFYLLNESVEIFNQMCDPRATEADVLSMISMSSEFDG IKFREEESKELKRLSDESVECQIGSQLDTPQGKANVLLQAYISQTRIFDSALSSDSNYVA QNSVRICRALFLIGVNRRWGKFSNVMLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDT KPSMEHLLELEADELGELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGP DFVWDSRIHGDAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDPLPPQV VVKVVSDTWIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSALQNPLIESIYPFKYF NPMQTMTFYTLYNTNENAFVGSPTGSGKTIVAELAIWHAFKTFPGKKIVYIAPMKALVRE RVDDWRKKITPVTGDKVVELTGDSLPDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDV SLIIMDEIHLLASDRGPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKD HGLYNFPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIFVASRR QTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHAGLVQ KDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMDLTDILQ MMGRAGRPAYDTTGTAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDDHLGAEITSGSIT NKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLENLRESQCVLLH GDDIVATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVEYNELPVRGGEII MNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDLPIADYIQDTVSVLDQ SLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGYWYEDDPVSVLPGLQLRRIKDYTFSE QGFIEMTPQQKKKKLLTLEEIGRFGYKKLLNVFDQLTFGMTESEDTKKRFVSVCQRLPVL EGMKFEEQENNEVLTFYSKHLSSKHNNGFEVYCDKFPKIQKELWFLIGHKGDELLMIKRC QPKQMNKEVIIHCDLFIPEEIRGEELQFSLINDALGLRYDMVHKLIS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
5904
Mutations:
None
Discrepancy:
c152a,P51Q; g577a,E193K;
c1312t,P438S; t4074c
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
