HFA1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148846.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00024289
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 6372nts
Open reading frame: 1 to 6372

ATGAGATCTATAAGAAAATGGGCGTACGAGACGTTCAATGATGAAAAAATCATTCAATTC
GTGGTAATGGCGACACCTGATGATTTACACGCAAATTCGGAGTATATTAGAATGGCAGAC
CAATATGTGCAGGTACCAGGGGGTACCAACAACAACAATTACGCCAACATAGACTTAATA
CTGGACGTGGCAGAGCAAACGGATGTGGATGCGGTCTGGGCTGGATGGGGCCATGCTTCT
GAAAATCCGTGTCTTCCTGAGCTGTTAGCTAGTTCACAAAGGAAAATACTATTCATTGGT
CCTCCTGGACGCGCTATGAGATCATTGGGTGACAAGATTTCTTCCACTATTGTAGCACAA
AGCGCTAAAATCCCGTGTATCCCTTGGTCTGGTTCACATATAGACACTATCCATATCGAT
AACAAGACGAACTTTGTATCTGTGCCGGATGATGTATATGTAAGGGGATGTTGTTCCTCA
CCTGAAGATGCTTTAGAAAAGGCTAAATTAATAGGATTTCCTGTAATGATTAAGGCATCC
GAAGGTGGTGGAGGTAAGGGCATTAGGCGAGTAGATAATGAGGATGATTTTATTGCATTA
TATCGCCAAGCAGTGAATGAGACACCTGGGTCGCCTATGTTTGTTATGAAAGTTGTCACT
GATGCTCGTCACTTAGAGGTACAGTTATTAGCTGACCAATATGGCACTAACATTACATTG
TTTGGGAGAGACTGTTCCATACAAAGGCGGCACCAAAAGATTATAGAAGAGGCACCAGTG
ACAATAACCAAGCCTGAAACGTTTCAAAGGATGGAACGCGCAGCAATTCGTCTAGGTGAA
TTGGTAGGTTATGTTTCTGCGGGCACTGTCGAATACTTATATTCACCAAAAGATGATAAA
TTTTACTTTTTAGAACTGAATCCGAGACTACAAGTAGAGCATCCAACGACAGAAATGATA
TCTGGCGTAAACCTTCCTGCCACTCAACTGCAAATCGCCATGGGCATTCCTATGCACATG
ATAAGTGATATCAGAAAACTTTATGGTTTAGATCCAACGGGAACTTCGTATATTGATTTT
AAAAATTTAAAGAGACCCTCGCCAAAAGGCCATTGTATTTCATGCAGGATCACTTCAGAA
GATCCTAATGAAGGTTTCAAGCCCTCCACTGGGAAAATACATGAGCTCAATTTTCGTTCT
TCTTCCAATGTTTGGGGTTACTTCTCAGTAGGAAATAATGGTGCTATTCACTCATTTTCA
GATTCCCAATTTGGGCACATTTTTGCTGTAGGAAACGATAGGCAAGATGCAAAGCAAAAC
ATGGTTTTAGCTCTAAAAGATTTTTCCATCCGAGGAGAATTCAAAACCCCCATAGAGTAC
CTGATAGAGCTATTAGAAACTCGGGACTTTGAGAGTAATAACATATCGACTGGTTGGTTA
GATGATTTGATTTTGAAAAATTTATCTTCCGATAGCAAACTAGATCCAACGCTCGCTATT
ATCTGTGGTGCCGCAATGAAAGCATACGTTTTCACAGAAAAGGTGAGGAATAAGTATTTG
GAATTATTGCGGAGGGGCCAAGTTCCACCTAAAGATTTTCTTAAAACGAAGTTTCCTGTT
GACTTCATTTTCGATAATAATAGATACTTGTTCAATGTTGCTCAATCATCTGAAGAACAA
TTTATTCTTTCTATCAATAAGTCTCAATGTGAAGTTAATGTTCAAAAATTGTCCAGTGAC
TGCTTGTTGATCTCCGTTGACGGTAAATGCCATACAGTTTATTGGAAGGACGATATCAGA
GGTACAAGACTTTCGATAGACTCCAATACCATATTTTTAGAAGCTGAACTCAATCCCACT
CAAGTGATCTCTCCAACTCCGGGGAAATTGGTGAAATATTTGGTCCGAAGTGGTGATCAC
GTTTTTGCTGGACAGCAATATGCAGAAATAGAAATAATGAAAATGCAGATGCCACTAGTA
GCGAAAAGTGATGGTGTAATTGAGTTACTAAGACAGCCCGGTTCCATAATTGAGGCTGGT
GATGTCATCGCAAAATTGACTTTGGATTCGCCGTCCAAAGCTAACGAATCGTCTTTATAC
CGCGGAGAATTACCTGTTTTAGGTCCACCGCTAATAGAGGGTAGCCGACCAAACCATAAG
CTCAGAGTCTTAATAAATAGGTTAGAAAATATTCTCAATGGATATCATGAAAACTCTGGA
ATAGAAACTACTCTAAAAGAGTTGATAAAAATATTGAGAGATGGTAGGCTTCCTTATTCA
GAATGGGATTCCCAAATTTCTACGGTACGCAATAGACTACCAAGGCAATTGAATGAGGGG
CTGGGAAATCTAGTCAAGAAATCTGTTTCTTTTCCTGCAAAGGAACTGCACAAATTAATG
AAGCGCTACTTGGAAGAAAATACAAATGATCATGTAGTTTATGTTGCCTTACAGCCACTT
CTTAAAATTAGTGAAAGGTATAGCGAAGGTTTAGCTAATCACGAATGTGAAATTTTTTTA
AAGTTGATTAAAAAGTACTATGCTGTTGAGAAAATTTTTGAAAATCATGATATACATGAA
GAAAGAAACTTACTAAATCTGCGGAGGAAAGACCTTACAAACTTAAAAGAAATTTTGTGC
ATAAGTTTATCGCATGCTAACGTAGTCGCAAAGAACAAGTTAGTAACTGCAATATTGCAT
GAATACGAGCCATTGTGCCAGGATTCCTCTAAGATGTCTTTAAAATTCAGGGCTGTTATA
CATGATTTGGCAAGTTTGGAATCTAAGTGGGCTAAAGAGGTTGCTGTAAAGGCAAGATCA
GTGCTACTCAGAGGGATTTTCCCTCCCATAAAGAAAAGAAAAGAGCATATTAAAACTCTC
CTGCAATTGCACATAAAGGATACTGGTGCCGAAAACATTCACAGCAGGAACATATATTCC
TGTATGAGGGATTTTGGTAATTTAATACATTCAAATCTGATACAACTTCAGGATTTGTTC
TTTTTTTTTGGCCATCAAGATACGGCTCTTTCCAGTATAGCATCTGAAATTTATGCAAGG
TATGCCTACGGCAATTATCAATTAAAAAGTATTAAGATTCATAAAGGAGCGCCTGATTTA
CTAATGTCATGGCAATTCAGCTCATTAAGAAATTATTTAGTCAATTCTGATGGTGAGAGT
GATGAGTTTACAAAACTTTCTAAACCTCCCTCAACATCAGGTAAGAGCTCAGCAAATAGT
TTTGGTCTTCTTGTCAACATGCGTGCGCTTGAATCTCTGGAAAAGACATTAGACGAGGTA
TACGAACAAATTCATATTCCTGAGGAAAGACTTTCCAGCGGAGAGAACTCTCTTATTGTT
AATATTTTATCTCCTATTCGTTACAGAAGTGAAAATGATCTAATTAAAACTTTAAAAATT
AAACTTCATGAAAATGAGAGAGGTCTATCCAAGCTCAAGGTTAATCGTATTACATTTGCA
TTTATCGCCGCGAATGCGCCCGCTGTTAAATTTTACTCCTTTGATGGAACTACGTACGAT
GAAATCTCTCAAATAAGAAATATGGATCCATCCTATGAAGCACCGTTAGAGTTAGGAAAA
ATGTCGAACTATAAAATCAGATCACTACCTACATACGATAGTAGTATACGCATTTTTGAA
GGTATTAGCAAATTTACGCCGCTAGATAAAAGGTTCTTTGTCAGGAAAATCATAAATTCC
TTCATGTATAATGATCAAAAAACAACCGAAGAAAACTTGAAAGCGGAAATCAATGCTCAA
GTGGTTTATATGTTAGAACATCTAGGAGCAGTTGACATCTCAAATTCAGACTTGAATCAT
ATTTTTTTAAGTTTCAATACAGTTCTTAACATACCAGTACATCGTCTCGAGGAAATTGTG
AGTACAATTCTAAAGACTCACGAAACCAGATTGTTTCAAGAAAGAATCACAGATGTAGAA
ATTTGCATCTCTGTTGAGTGCCTAGAAACAAAGAAGCCAGCCCCGCTTAGATTACTTATT
TCTAATAAATCTGGGTATGTGGTAAAAATTGAGACATATTACGAAAAGATAGGGAAAAAT
GGGAATCTGATTTTGGAACCGTGTAGTGAGCAGAGCCATTATAGCCAGAAATCTCTCTCT
CTTCCTTACTCGGTCAAGGATTGGCTACAACCTAAAAGGTACAAAGCTCAATTCATGGGT
ACAACATATGTGTACGATTTCCCAGGTCTGTTTCATCAAGCTGCAATCCAACAGTGGAAA
AGGTATTTTCCAAAACATAAGCTGAATGACAGTTTTTTTAGTTGGGTTGAATTGATAGAA
CAAAACGGTAATTTAATAAAAGTAAACAGGGAGCCAGGCCTTAATAATATAGGGATGGTT
GCTTTTGAGATTATGGTTCAGACACCTGAATATCCTGAAGGGCGTAACATGATCGTGATT
TCTAATGATATTACCTACAATATTGGATCTTTTGGACCGAGAGAAGATTTGTTTTTTGAT
AGGGTCACAAATTATGCAAGAGAGAGAGGGATCCCGAGGATATACTTGGCGGCGAATTCA
GGAGCTAAATTGGGTATAGCCGAAGAGCTGATCCCTCTATTTCGTGTAGCATGGAATGAC
CCCTCTGATCCAACAAAGGGTTTCCAGTACTTATACTTAGCTCCAAAAGACATGCAGCTA
CTGAAAGATTCTGGGAAAGGAAATTCGGTTGTTGTTGAACACAAGATGGTATACGGTGAA
GAGAGATATATTATTAAAGCAATAGTCGGATTCGAAGAGGGTTTAGGTGTTGAATGTTTA
CAGGGCTCGGGTTTAATTGCTGGTGCCACTTCGAAAGCGTATAGAGACATTTTCACTATT
ACTGCTGTTACTTGTCGGTCCGTTGGTATAGGTTCCTATCTGGTCAGACTAGGACAACGT
ACTATTCAGGTGGAGGATAAGCCTATCATACTGACGGGTGCATCGGCGATTAATAAAGTT
TTGGGTACCGATATCTATACATCTAACCTACAAATTGGCGGAACCCAAATCATGTATAAA
AACGGAATAGCGCATTTAACAGCCAGTAATGATATGAAAGCCATCGAAAAAATAATGACA
TGGTTATCATATGTCCCGGCGAAAAGAGATATGAGTCCTCCACTTCTTGAAACTATGGAT
AGATGGGATAGGGATGTAGACTTCAAACCTGCCAAGCAAGTGCCATATGAGGCAAGGTGG
TTGATAGAGGGTAAATGGGACTCAAATAACAACTTCCAGTCAGGCCTATTTGATAAGGAT
TCGTTTTTTGAGACATTATCTGGATGGGCCAAAGGTGTAATAGTTGGAAGAGCACGTCTT
GGAGGTATTCCCGTGGGTGTTATTGCGGTAGAAACTAAGACTATCGAAGAAATAATCCCC
GCTGACCCAGCTAATCTGGATTCTTCAGAGTTTTCCGTTAAAGAAGCAGGACAGGTGTGG
TATCCAAATTCCGCGTTCAAAACAGCTCAAACTATAAATGATTTTAACTATGGTGAGCAA
TTACCATTGATTATCTTAGCCAATTGGAGGGGATTTTCTGGCGGTCAAAGGGATATGTAC
AATGAAGTACTAAAGTACGGGTCTTTTATTGTTGACGCTCTGGTTGACTACAAACAACCC
ATACTGATATACATTCCGCCCTTTGGTGAATTAAGGGGCGGATCATGGGTTGTTATAGAT
CCAACTATTAATCCTGAACAAATGGAAATGTATGCCGATGTTGAATCTAGGGGAGGTGTG
TTAGAACCTGACGGAGTAGTTAGCATAAAATACCGTAAGGAGAAAATGATAGAGACGATG
ATTCGATTAGACTCCACATATGGACATTTGAGAAGAACGTTGACAGAAAAAAAGTTATCT
TTGGAAAAACAAAATGATCTTACGAAGAGATTGAAAATAAGAGAGAGACAGTTGATACCA
ATTTACAATCAAATCAGCATACAGTTTGCAGATTTACATGATAGATCGACTAGGATGCTA
GTTAAAGGAGTAATCCGAAATGAGTTGGAATGGAAAAAGTCACGCAGATTTTTATATTGG
AGACTGAGAAGGAGGTTGAACGAGGGACAAGTGATCAAAAGACTGCAAAAAAAAACATGT
GATAACAAAACGAAAATGAAGTACGACGACCTGTTGAAAATAGTTCAGTCATGGTATAAC
GATCTGGATGTTAATGATGACAGAGCAGTAGTGGAGTTCATAGAAAGAAATTCGAAAAAA
ATTGACAAGAACATTGAAGAGTTTGAGATCTCGCTGTTGATCGATGAGCTTAAGAAAAAA
TTTGAAGACAGAAGGGGAAACATTGTCCTTGAAGAGCTAACTAGGTTGGTGGACAGTAAG
CGAAAGAGATAG

Protein sequence predicted based on nucleotide sequence

MRSIRKWAYETFNDEKIIQFVVMATPDDLHANSEYIRMADQYVQVPGGTNNNNYANIDLI
LDVAEQTDVDAVWAGWGHASENPCLPELLASSQRKILFIGPPGRAMRSLGDKISSTIVAQ
SAKIPCIPWSGSHIDTIHIDNKTNFVSVPDDVYVRGCCSSPEDALEKAKLIGFPVMIKAS
EGGGGKGIRRVDNEDDFIALYRQAVNETPGSPMFVMKVVTDARHLEVQLLADQYGTNITL
FGRDCSIQRRHQKIIEEAPVTITKPETFQRMERAAIRLGELVGYVSAGTVEYLYSPKDDK
FYFLELNPRLQVEHPTTEMISGVNLPATQLQIAMGIPMHMISDIRKLYGLDPTGTSYIDF
KNLKRPSPKGHCISCRITSEDPNEGFKPSTGKIHELNFRSSSNVWGYFSVGNNGAIHSFS
DSQFGHIFAVGNDRQDAKQNMVLALKDFSIRGEFKTPIEYLIELLETRDFESNNISTGWL
DDLILKNLSSDSKLDPTLAIICGAAMKAYVFTEKVRNKYLELLRRGQVPPKDFLKTKFPV
DFIFDNNRYLFNVAQSSEEQFILSINKSQCEVNVQKLSSDCLLISVDGKCHTVYWKDDIR
GTRLSIDSNTIFLEAELNPTQVISPTPGKLVKYLVRSGDHVFAGQQYAEIEIMKMQMPLV
AKSDGVIELLRQPGSIIEAGDVIAKLTLDSPSKANESSLYRGELPVLGPPLIEGSRPNHK
LRVLINRLENILNGYHENSGIETTLKELIKILRDGRLPYSEWDSQISTVRNRLPRQLNEG
LGNLVKKSVSFPAKELHKLMKRYLEENTNDHVVYVALQPLLKISERYSEGLANHECEIFL
KLIKKYYAVEKIFENHDIHEERNLLNLRRKDLTNLKEILCISLSHANVVAKNKLVTAILH
EYEPLCQDSSKMSLKFRAVIHDLASLESKWAKEVAVKARSVLLRGIFPPIKKRKEHIKTL
LQLHIKDTGAENIHSRNIYSCMRDFGNLIHSNLIQLQDLFFFFGHQDTALSSIASEIYAR
YAYGNYQLKSIKIHKGAPDLLMSWQFSSLRNYLVNSDGESDEFTKLSKPPSTSGKSSANS
FGLLVNMRALESLEKTLDEVYEQIHIPEERLSSGENSLIVNILSPIRYRSENDLIKTLKI
KLHENERGLSKLKVNRITFAFIAANAPAVKFYSFDGTTYDEISQIRNMDPSYEAPLELGK
MSNYKIRSLPTYDSSIRIFEGISKFTPLDKRFFVRKIINSFMYNDQKTTEENLKAEINAQ
VVYMLEHLGAVDISNSDLNHIFLSFNTVLNIPVHRLEEIVSTILKTHETRLFQERITDVE
ICISVECLETKKPAPLRLLISNKSGYVVKIETYYEKIGKNGNLILEPCSEQSHYSQKSLS
LPYSVKDWLQPKRYKAQFMGTTYVYDFPGLFHQAAIQQWKRYFPKHKLNDSFFSWVELIE
QNGNLIKVNREPGLNNIGMVAFEIMVQTPEYPEGRNMIVISNDITYNIGSFGPREDLFFD
RVTNYARERGIPRIYLAANSGAKLGIAEELIPLFRVAWNDPSDPTKGFQYLYLAPKDMQL
LKDSGKGNSVVVEHKMVYGEERYIIKAIVGFEEGLGVECLQGSGLIAGATSKAYRDIFTI
TAVTCRSVGIGSYLVRLGQRTIQVEDKPIILTGASAINKVLGTDIYTSNLQIGGTQIMYK
NGIAHLTASNDMKAIEKIMTWLSYVPAKRDMSPPLLETMDRWDRDVDFKPAKQVPYEARW
LIEGKWDSNNNFQSGLFDKDSFFETLSGWAKGVIVGRARLGGIPVGVIAVETKTIEEIIP
ADPANLDSSEFSVKEAGQVWYPNSAFKTAQTINDFNYGEQLPLIILANWRGFSGGQRDMY
NEVLKYGSFIVDALVDYKQPILIYIPPFGELRGGSWVVIDPTINPEQMEMYADVESRGGV
LEPDGVVSIKYRKEKMIETMIRLDSTYGHLRRTLTEKKLSLEKQNDLTKRLKIRERQLIP
IYNQISIQFADLHDRSTRMLVKGVIRNELEWKKSRRFLYWRLRRRLNEGQVIKRLQKKTC
DNKTKMKYDDLLKIVQSWYNDLDVNDDRAVVEFIERNSKKIDKNIEEFEISLLIDELKKK
FEDRRGNIVLEELTRLVDSKRKR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 6372
Mutations:
None
Discrepancy:
g2625a; a2629g,K877E
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable