Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH202745.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00025085
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3276nts
Open reading frame: 1 to 3276
ATGGATAAAAGTAAGCAGATGAACATCAACAACTTGTCCAACATCCCCGAGGTGATTGAC
CCTGGCATCACTATCCCAATATACGAGGAAGAATACGAGAACAATGGAGAGAGTAATAGC
CAACTGCAGCAACAACCCCAAAAGCTTGGATCATATAGGTCGCGAGCAGGAAAATTTTCT
AATACATTGAGTAACCTTTTGCCCTCCATCAGTGCCAAGTTACACCATTCGAAGAAAAAT
AGTCACGGAAAGAACGGTGCTGAGTTTTCCTCTAGCAACAACAGTTCCCAATCTACGGTG
GCGTCCAAGACACCAAGGGCCAGCCCTTCCAGGAGTAAGATGATGGAAAGTTCTATAGAC
GGTGTAACTATGGATAGACCTGGCAGTTTAACCCCTCCTCAAGACATGGAAAAATTGGTG
CATTTTCCTGACTCGAGTAATAACTTCTTAATTCCTGCCCCCCGAGGATCTAGCGACTCG
TTTAACTTGCCACATCAGATCTCGAGGACGAGAAACAACACAATGTCTTCTCAGATAACT
TCCATTTCATCAATCGCCCCCAAGCCTCGTACTTCGAGCGGAATATGGTCTTCAAATGCT
AGTGCGAACGATCCCATGCAACAGCACCTTTTGCAACAATTGCAACCTACGACAAGCAAT
AATACAACTAATTCAAACACGCTCAACGATTATTCGACAAAAACTGCATATTTTGACAAC
ATGGTATCTACTAGTGGAAGCCAAATGGCAGATAATAAGATGAATACGAATAACTTGGCT
ATCCCAAACTCTGTTTGGTCAAATACCAGACAGAGATCTCAATCCAACGCGTCTAGCATA
TATACCGACGCTCCACTGTACGAACAGCCCGCTCGTGCCAGTATTTCATCGCACTATACT
ATTCCAACACAGGAATCTCCATTGATTGCTGATGAAATAGATCCGCAATCCATCAATTGG
GTAACAATGGACCCCACTGTACCATCAATAAACCAAATATCGAACCTTTTACCCACAAAT
ACCATTTCTATTTCCAACGTTTTTCCCCTACAGCACCAGCAGCCACAATTAAATAACGCG
ATAAACTTAACAAGTACTTCCTTGGCAACCCTATGCTCCAAATATGGTGAAGTCATATCT
GCTAGGACTTTAAGGAATTTAAACATGGCTTTAGTTGAATTTTCTTCCGTGGAAAGTGCC
GTAAAAGCCTTAGATAGTTTACAGGGCAAGGAGGTATCTATGATTGGAGCACCAAGTAAA
ATTTCATTTGCCAAAATCTTACCTATGCATCAACAACCCCCACAATTTCTTCTCAATTCT
CAAGGCTTGCCATTGGGTTTAGAAAACAACAATTTGCAACCACAGCCTTTATTACAGGAG
CAGTTATTCAATGGAGCTGTGACTTTCCAACAACAAGGAAATGTTTCTATACCTGTATTT
AACCAGCAATCACAACAATCGCAGCACCAAAACCATAGTTCGGGTTCTGCTGGTTTTAGC
AATGTTCTCCATGGTTATAATAATAACAATAGTATGCACGGAAACAACAATAACTCAGCA
AATGAAAAAGAACAATGTCCTTTCCCTCTACCACCACCTAACGTTAACGAAAAGGAAGAT
TTGTTAAGAGAGATCATCGAACTTTTTGAAGCAAATTCCGATGAATACCAAATCAATTCG
TTGATAAAAAAATCGCTGAATCACAAAGGCACTTCCGATACGCAAAACTTTGGACCTTTA
CCAGAACCATTGTCTGGCAGGGAGTTCGATCCACCAAAATTACGCGAATTGCGAAAATCA
ATCGATTCAAACGCATTTTCCGACTTAGAGATCGAACAGTTGGCTATTGCAATGCTTGAC
GAATTGCCCGAATTGAGTTCAGATTACTTGGGTAACACTATCGTTCAAAAATTATTTGAA
CATTCATCTGATATTATTAAAGACATCATGCTGAGGAAAACAAGCAAATACCTAACCTCT
ATGGGTGTTCACAAAAACGGAACATGGGCTTGTCAGAAGATGATCACTATGGCGCATACT
CCGAGACAAATCATGCAAGTGACGCAAGGGGTCAAGGACTACTGTACTCCATTAATCAAT
GACCAATTCGGAAATTATGTCATACAGTGCGTTTTGAAATTTGGATTCCCCTGGAACCAG
TTTATTTTTGAAAGTATAATAGCTAATTTTTGGGTTATTGTTCAGAATAGATATGGTGCA
AGGGCAGTCAGAGCTTGTTTAGAGGCACATGACATTGTAACTCCTGAACAATCAATAGTT
TTGAGTGCAATGATCGTTACATATGCTGAATATCTAAGCACTAATAGCAATGGTGCGTTG
TTAGTAACGTGGTTTTTAGATACTAGTGTTTTACCCAACAGACATTCTATCTTGGCACCA
AGATTGACGAAAAGAATTGTAGAGTTATGTGGCCACAGATTGGCTTCTTTGACTATTTTG
AAGGTCTTGAATTACAGAGGGGATGATAACGCTAGAAAAATCATCCTAGATTCCCTATTT
GGAAACGTGAACGCACACGATTCTTCGCCACCAAAGGAATTAACAAAGCTTTTATGTGAA
ACAAATTATGGCCCAACCTTTGTCCATAAAGTTTTAGCAATGCCATTATTAGAAGATGAC
TTAAGAGCCCACATCATTAAACAAGTCAGAAAAGTATTGACGGATTCTACTCAAATCCAA
CCAAGCCGCCGTTTATTGGAAGAAGTCGGGTTGGCATCACCCTCTAGTACTCATAACAAG
ACAAAACAACAACAGCAGCAGCATCATAACAGCAGCATTTCGCACATGTTCGCCACTCCT
GATACTTCTGGACAACACATGAGAGGTCTTTCTGTTTCTAGCGTTAAAAGTGGTGGATCT
AAACATACCACAATGAACACCACGACTACAAATGGTTCTTCTGCCTCTACATTATCCCCG
GGCCAACCATTGAATGCCAATTCTAATTCTTCAATGGGCTATTTTAGTTATCCTGGCGTC
TTCCCTGTCAGCGGCTTTTCAGGCAACGCCAGTAATGGATATGCTATGAACAACGATGAC
TTAAGTTCCCAGTTTGATATGCTAAACTTTAATAACGGAACCCGTTTATCGTTGCCACAA
CTGAGCCTAACTAACCATAACAACACTACTATGGAATTAGTAAACAATGTTGGCTCCTCA
CAACCTCATACGAACAACAACAACAATAATAACAACACGAACTATAACGACGATAACACC
GTTTTTGAAACTCTGACTTTACACTCTGCAAACTAG
Protein sequence predicted based on nucleotide sequence
MDKSKQMNINNLSNIPEVIDPGITIPIYEEEYENNGESNSQLQQQPQKLGSYRSRAGKFS NTLSNLLPSISAKLHHSKKNSHGKNGAEFSSSNNSSQSTVASKTPRASPSRSKMMESSID GVTMDRPGSLTPPQDMEKLVHFPDSSNNFLIPAPRGSSDSFNLPHQISRTRNNTMSSQIT SISSIAPKPRTSSGIWSSNASANDPMQQHLLQQLQPTTSNNTTNSNTLNDYSTKTAYFDN MVSTSGSQMADNKMNTNNLAIPNSVWSNTRQRSQSNASSIYTDAPLYEQPARASISSHYT IPTQESPLIADEIDPQSINWVTMDPTVPSINQISNLLPTNTISISNVFPLQHQQPQLNNA INLTSTSLATLCSKYGEVISARTLRNLNMALVEFSSVESAVKALDSLQGKEVSMIGAPSK ISFAKILPMHQQPPQFLLNSQGLPLGLENNNLQPQPLLQEQLFNGAVTFQQQGNVSIPVF NQQSQQSQHQNHSSGSAGFSNVLHGYNNNNSMHGNNNNSANEKEQCPFPLPPPNVNEKED LLREIIELFEANSDEYQINSLIKKSLNHKGTSDTQNFGPLPEPLSGREFDPPKLRELRKS IDSNAFSDLEIEQLAIAMLDELPELSSDYLGNTIVQKLFEHSSDIIKDIMLRKTSKYLTS MGVHKNGTWACQKMITMAHTPRQIMQVTQGVKDYCTPLINDQFGNYVIQCVLKFGFPWNQ FIFESIIANFWVIVQNRYGARAVRACLEAHDIVTPEQSIVLSAMIVTYAEYLSTNSNGAL LVTWFLDTSVLPNRHSILAPRLTKRIVELCGHRLASLTILKVLNYRGDDNARKIILDSLF GNVNAHDSSPPKELTKLLCETNYGPTFVHKVLAMPLLEDDLRAHIIKQVRKVLTDSTQIQ PSRRLLEEVGLASPSSTHNKTKQQQQQHHNSSISHMFATPDTSGQHMRGLSVSSVKSGGS KHTTMNTTTTNGSSASTLSPGQPLNANSNSSMGYFSYPGVFPVSGFSGNASNGYAMNNDD LSSQFDMLNFNNGTRLSLPQLSLTNHNNTTMELVNNVGSSQPHTNNNNNNNNTNYNDDNT VFETLTLHSAN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3276
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
