KAP120 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202908.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00025141
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3099nts
Open reading frame: 1 to 3099

ATGGCCTCGTCTCTTAACGAGTTAAACTTGGTTCAAGTACTTGAACAAGCAAGTAACCCA
CAACATATTAGGTCAGATGTTCAAAAATTAGCCGAACAACAACTAAGACAATGGGAGACT
CAAGCGGGATTTCATTACTTATTACAGTCAATCTACCTTAATTTATCTAATTCTTTACAA
ATTAGATGGTTGGCGGTAATTCAATTTAAGAACGGCGTGGATAAATACTGGAGATCCACT
AGAATTAACGCTATACCTAAAGATGAAAAAGCCTCGATAAGAGGTCGTCTTTTTGAAATG
ATCGATGAACAGAATAATCAACTTTGTATTCAGAATGCCCAAGCTTCCGCAAGGATTGCA
AGGCTAGATTTTCCCGTAGAGTGGCCAACCTTATTTGAGGATCTAGAAAACCTATTGAAC
GATGAGATCATCAGAAAAGATTCAGTTAAGATTTACAATATATTGATGCATATAAATCAA
ATCGTTAAAGTATTAGGTACTGCGAGAATAGGAAGGTGCCGACCCGCTATGCAAAGTAAA
GTTCCTTTGATATTACCACTAATTGTCAGGATTTATTTACAATCATTTGAAGAATGGACG
ACATCCTCCAATCTCAACTACGAGGATCTTTCCAGTTTACAAGTTTCTTACCTAGCTTTG
AAAGTGCTCAGAAGGATAATTTGTGAAGGATACGACCGCCCTCAAACCGATCAGTCTGTT
TGCGATTTTATTAAACTATCCGTTTCACACTTTGAGATGTTAATTTCAAACCATGAAAAC
TTCAAAAAGTTTGATATTTACGAAAAGTTTATCAAATGCCTAGGAAAGTTATATTTCAAT
TTAGTCACTGGCTCTCCGGCTAATTTCATATTACTGCCATGCTCTACGCAAATTCTAATC
ACTTATACTAGGCTAATTTTCGATAAGGCCCCTAAAGTATATAGAGAGAATTCTGATGTC
ACGGGGGACTTTTGGGAACAGACTGCTATTAGAGGGCTTTTAATTTTGAAAAGGGTCATT
AATTTCATTCACAAAAAGGGGGCAATTACGTTAAAAGCAAGAAGTGATAAACTAACCATA
GATGCTTCTATCAACAAAATCAACACAGAATTTCTGAATGAGAATTTGATAACAAGGTTG
GTTGACACTTTAATGGAATGGTACTTGAGGTTAAGACCAACTGAATTAGAAAATTGGTTT
ATGGATCCAGAAGAATGGATAAACGAACAAATGGCCACGAGTTACGAATATCAAATCAGA
CCATGTGCAGAGAACGTTTTTCAAGACTTAATGAATACTTTCTCTGAGCTGTTGGTTCCC
TACCTGTTAAAGAAAATTGAAAATGATGCTTCAAAGCTATCAAACTCATTAGACGATTTT
TTAAGGAAAGACGCAATTTATGCAAGTTTCCAATTAAGTGCATCCGCTGTTAGTGAAATG
GTAGATTTTGACAGGTTACTAATTCAGGTTTTTTTACCAGAAGCTACAAATACAAACATA
TCAGGCGACGAATTAAGGATTATCAGAAGAAGAGTTGCTTTAATTATAAACGAATGGTCT
ACTGTGAAGTGTTCGGAAGAATCTAAAAGTTTATGTTACAAGTTATTTACAAACTTCCTG
ACAGATGAAGACGACAAAGTTGTTTTGTTAACCACAGTCCAGACTGTCAGAACCATGGTT
GATGACTGGAACTTCAACAAGGATACTTTTCAGCCATTTTTAACTGAAAATGTTCATTTA
TTGTTGAGGAAAATTTTACCATCTGTGTCGTTAACCGAGACAAGGCTATATGTTTTAAAC
ACCTTGAGCGATATCATAATTCAAACAAAACCTTTAATTAGCAGGGATCTATTAGTCGAA
ATTTTGCAAATCATACCAAACCTTTGGGAAATAGCAACAAATAATGCTAGTGAGGCTATT
TTGGCAAACGCATTGTTAAGGTTATTGAGAAATTTGGTATCATCTTTGGGATCACAGTCA
CATTTAACGTGGGATATCGCCATACCTGTGGTTGCATTAGCCTGTGATCCCTCTTCTATG
CAATATCAGTTGTTAAGCGAAGACGGGTATGAACTATGGGGCATGCTTCTTCAAAATTTT
TCATCCCACGATCAAGAGTTTGACGATAAGTTCGTTGAATTGGTGCCATTTCTAAAGTAT
GGTATTGAGACACACACAGAGATTCTCCCAACTTTACTTGAAATTATCAAAAGTTATGCT
CTGATTCTGAATCCTGTGGATTTTTTCTCTAACAACACATTTCAAGATATTTTTAAACAA
ATGTCTAAATATCTTTTAAAGTTAAGGGAAGATTCATTTCAGTTGGTTTTAGAAATCTGG
GAAATTTTGATATTATCCAATGAGTCGGATTACGAAAATCTGTTATTGCAAAAATTCTAT
GAAACCGGAGTATTATCAGCATTATTCGATGCCATTTTCCTGGAAGAAGCTCCTTCATCT
TATTTATGCTCGCAAATCATTCAAATCATCGCTAGAATATCCTACGTGAATCCAGACGCC
TTAATGACTTTTTTAGCTACATATCATGACAATCTACCAACTTCTAACGAAAATGCACGC
ATGCCGGAATCAATAAGAAAAATTGTGTCTAAGGATCAGACGTATGATTCCGTTGTAAAC
AAGCTTTTAACCGGATGGATAGTATGTTTTAGAGATATCTTTGATCCTAAATTCAAAAAA
GTTCACATATTAGGTATTTCAAGTTTACTAAGGACTGGTTTAGTTCCTATTTTGACTGAG
TTTTCATCCATCGCATCTTTATGGATCGAAATGCTCGAAGAAATTAATGAAACTAATCGT
GGGGATTGCGAAAAATACCACTTGAATGATATTGTTACAGAGCAATCAATTGCATTCCAT
CCTTTAACTGCCGAACAATTAAGATATCATCAATTATGCAAAAACAATGATCCAGTGCAT
AACATTAGTTTAAAAGATTTTATTTCCCAATCAATGGAATATCTAGAATCACATTTAGGA
GTAGAAAGGTACCAAGAATTTTTGAAGACTATTAACCCTAGTTTACTAGAGAACTTACAA
ATGTTTTTATCCATCCAGCCTCAAGAGGCTCGTCCATAG

Protein sequence predicted based on nucleotide sequence

MASSLNELNLVQVLEQASNPQHIRSDVQKLAEQQLRQWETQAGFHYLLQSIYLNLSNSLQ
IRWLAVIQFKNGVDKYWRSTRINAIPKDEKASIRGRLFEMIDEQNNQLCIQNAQASARIA
RLDFPVEWPTLFEDLENLLNDEIIRKDSVKIYNILMHINQIVKVLGTARIGRCRPAMQSK
VPLILPLIVRIYLQSFEEWTTSSNLNYEDLSSLQVSYLALKVLRRIICEGYDRPQTDQSV
CDFIKLSVSHFEMLISNHENFKKFDIYEKFIKCLGKLYFNLVTGSPANFILLPCSTQILI
TYTRLIFDKAPKVYRENSDVTGDFWEQTAIRGLLILKRVINFIHKKGAITLKARSDKLTI
DASINKINTEFLNENLITRLVDTLMEWYLRLRPTELENWFMDPEEWINEQMATSYEYQIR
PCAENVFQDLMNTFSELLVPYLLKKIENDASKLSNSLDDFLRKDAIYASFQLSASAVSEM
VDFDRLLIQVFLPEATNTNISGDELRIIRRRVALIINEWSTVKCSEESKSLCYKLFTNFL
TDEDDKVVLLTTVQTVRTMVDDWNFNKDTFQPFLTENVHLLLRKILPSVSLTETRLYVLN
TLSDIIIQTKPLISRDLLVEILQIIPNLWEIATNNASEAILANALLRLLRNLVSSLGSQS
HLTWDIAIPVVALACDPSSMQYQLLSEDGYELWGMLLQNFSSHDQEFDDKFVELVPFLKY
GIETHTEILPTLLEIIKSYALILNPVDFFSNNTFQDIFKQMSKYLLKLREDSFQLVLEIW
EILILSNESDYENLLLQKFYETGVLSALFDAIFLEEAPSSYLCSQIIQIIARISYVNPDA
LMTFLATYHDNLPTSNENARMPESIRKIVSKDQTYDSVVNKLLTGWIVCFRDIFDPKFKK
VHILGISSLLRTGLVPILTEFSSIASLWIEMLEEINETNRGDCEKYHLNDIVTEQSIAFH
PLTAEQLRYHQLCKNNDPVHNISLKDFISQSMEYLESHLGVERYQEFLKTINPSLLENLQ
MFLSIQPQEARP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3099
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable