SLA2 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202974.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00025154
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2907nts
Open reading frame: 1 to 2907

ATGTCCAGAATAGATTCAGATCTGCAGAAAGCGCTTAAAAAGGCGTGTTCCGTTGAGGAG
ACCGCACCTAAGAGAAAGCACGTACGCGCATGCATAGTGTACACATGGGACCATCAGTCT
TCCAAAGCTGTTTTTACAACCCTTAAGACGCTGCCTCTGGCCAATGACGAAGTGCAACTG
TTCAAGATGCTTATTGTACTGCATAAGATCATACAAGAGGGACATCCGTCAGCGCTAGCT
GAGGCCATCCGGGATAGGGACTGGATCAGATCTCTGGGTAGGGTACATTCTGGCGGTTCG
TCGTACAGCAAACTGATTCGGGAGTATGTGCGGTACCTGGTTTTGAAGCTGGACTTTCAT
GCGCACCATAGAGGGTTTAACAACGGGACGTTTGAGTACGAGGAGTATGTGTCACTGGTT
TCAGTTTCCGACCCGGACGAAGGGTACGAGACGATCTTAGATTTGATGTCGTTACAGGAT
TCTCTTGACGAGTTCTCGCAGATCATCTTTGCGTCGATTCAGTCCGAGAGGAGAAATACT
GAGTGCAAAATTTCGGCGCTGATCCCGTTGATTGCGGAGTCATATGGTATTTATAAATTC
ATCACCTCGATGCTGAGAGCTATGCACAGGCAATTGAATGATGCGGAGGGTGACGCTGCT
CTGCAGCCCTTGAAAGAACGGTACGAGTTGCAACATGCAAGGCTGTTTGAGTTTTACGCG
GACTGCTCTTCTGTGAAGTACTTGACCACGTTAGTAACCATTCCAAAACTACCCGTGGAC
GCTCCAGACGTATTTTTAATCAACGATGTGGACGAGTCAAAAGAGATTAAGTTCAAAAAA
AGAGAGCCCTCTGTAACTCCAGCGCGGACGCCGGCACGGACTCCAACACCAACACCACCT
GTTGTTGCTGAGCCGGCTATTTCCCCACGTCCGGTATCTCAAAGGACTACTTCGACGCCT
ACGGGTTACTTGCAAACTATGCCGACGGGTGCCACCACTGGGATGATGATCCCCACTGCC
ACTGGTGCAGCTAACGCCATTTTTCCACAGGCGACGGCACAAATGCAGCCGGACTTCTGG
GCCAATCAACAAGCCCAGTTTGCGAACGAGCAAAACCGTCTCGAGCAAGAGCGTGTGCAA
CAGTTGCAACAACAGCAGGCACAGCAGGAACTGTTTCAACAGCAGTTACAAAAAGCACAA
CAGGATATGATGAATATGCAGCTTCAACAACAAAACCAACATCAAAACGATTTAATTGCC
CTCACCAATCAATATGAAAAGGACCAGGCTTTATTGCAGCAATATGACCAAAGAGTGCAG
CAGCTGGAAAGTGAAATCACAACGATGGATTCTACAGCTTCCAAACAATTGGCTAATAAG
GACGAGCAACTAACTGCATTGCAGGATCAATTGGATGTTTGGGAAAGAAAATATGAGTCT
TTAGCCAAGCTATACTCCCAGTTGCGTCAAGAGCATCTAAATCTTTTACCTCGTTTTAAA
AAACTGCAGTTAAAGGTGAATAGTGCGCAGGAATCCATTCAGAAAAAGGAACAGTTAGAA
CACAAATTGAAGCAAAAGGATTTGCAAATGGCCGAGTTGGTTAAGGACCGTGACAGGGCA
AGGTTAGAATTAGAAAGATCCATTAATAATGCAGAGGCGGACAGTGCAGCGGCGACAGCA
GCAGCAGAAACAATGACTCAGGATAAAATGAACCCTATTCTGGATGCCATATTGGAAAGC
GGTATCAACACCATCCAGGAATCGGTCTATAATCTCGATTCTCCTTTGAGTTGGTCGGGT
CCGCTGACTCCACCCACTTTCCTTTTGTCCTTATTGGAAAGTACTTCTGAAAACGCCACT
GAGTTTGCCACCAGTTTCAATAATTTGATAGTAGACGGCCTTGCACACGGCGACCAAACA
GAGGTCATACACTGCGTTAGTGATTTCAGTACTTCGATGGCTACACTGGTGACCAATTCA
AAGGCGTACGCCGTTACTACATTACCTCAAGAACAGTCTGATCAAATTTTGACCCTGGTG
AAGAGATGTGCCAGAGAGGCCCAATATTTCTTCGAGGATTTGATGTCTGAAAATCTTAAC
CAGGTCGGTGATGAGGAGAAAACTGATATCGTCATCAATGCCAACGTGGACATGCAAGAG
AAGTTGCAAGAGCTATCTTTGGCTATCGAACCGTTATTGAACATACAATCTGTCAAATCA
AATAAAGAAACGAACCCACATTCTGAATTGGTTGCCACTGCTGACAAAATTGTCAAATCC
TCAGAACACTTACGTGTTGACGTTCCTAAACCATTGTTGTCATTGGCATTAATGATTATT
GATGCTGTTGTGGCTTTGGTAAAGGCTGCTATTCAGTGCCAAAATGAAATTGCCACCACG
ACAAGTATTCCTCTGAACCAATTCTACTTGAAAAACAGCAGATGGACAGAAGGTTTGATC
TCCGCGGCAAAGGCTGTGGCAGGTGCAACCAATGTTTTAATCACTACTGCTAGTAAGTTA
ATCACTTCTGAAGACAATGAAAACACATCACCTGAACAATTTATCGTCGCCTCCAAAGAA
GTGGCCGCATCCACAATACAATTGGTAGCAGCATCGAGAGTGAAAACTTCCATCCACTCG
AAGGCCCAAGATAAATTGGAACACTGTTCCAAAGATGTTACCGATGCATGCAGAAGTTTG
GGTAACCACGTCATGGGTATGATTGAAGATGATCATTCGACTTCGCAACAGCAGCAACCA
TTGGACTTCACATCCGAACACACATTGAAGACTGCCGAAATGGAACAGCAAGTGGAAATT
TTGAAGTTAGAACAATCCTTGAGCAATGCCAGGAAGAGATTGGGCGAGATAAGAAGGCAT
GCCTACTATAACCAGGATGATGATTAG

Protein sequence predicted based on nucleotide sequence

MSRIDSDLQKALKKACSVEETAPKRKHVRACIVYTWDHQSSKAVFTTLKTLPLANDEVQL
FKMLIVLHKIIQEGHPSALAEAIRDRDWIRSLGRVHSGGSSYSKLIREYVRYLVLKLDFH
AHHRGFNNGTFEYEEYVSLVSVSDPDEGYETILDLMSLQDSLDEFSQIIFASIQSERRNT
ECKISALIPLIAESYGIYKFITSMLRAMHRQLNDAEGDAALQPLKERYELQHARLFEFYA
DCSSVKYLTTLVTIPKLPVDAPDVFLINDVDESKEIKFKKREPSVTPARTPARTPTPTPP
VVAEPAISPRPVSQRTTSTPTGYLQTMPTGATTGMMIPTATGAANAIFPQATAQMQPDFW
ANQQAQFANEQNRLEQERVQQLQQQQAQQELFQQQLQKAQQDMMNMQLQQQNQHQNDLIA
LTNQYEKDQALLQQYDQRVQQLESEITTMDSTASKQLANKDEQLTALQDQLDVWERKYES
LAKLYSQLRQEHLNLLPRFKKLQLKVNSAQESIQKKEQLEHKLKQKDLQMAELVKDRDRA
RLELERSINNAEADSAAATAAAETMTQDKMNPILDAILESGINTIQESVYNLDSPLSWSG
PLTPPTFLLSLLESTSENATEFATSFNNLIVDGLAHGDQTEVIHCVSDFSTSMATLVTNS
KAYAVTTLPQEQSDQILTLVKRCAREAQYFFEDLMSENLNQVGDEEKTDIVINANVDMQE
KLQELSLAIEPLLNIQSVKSNKETNPHSELVATADKIVKSSEHLRVDVPKPLLSLALMII
DAVVALVKAAIQCQNEIATTTSIPLNQFYLKNSRWTEGLISAAKAVAGATNVLITTASKL
ITSEDNENTSPEQFIVASKEVAASTIQLVAASRVKTSIHSKAQDKLEHCSKDVTDACRSL
GNHVMGMIEDDHSTSQQQQPLDFTSEHTLKTAEMEQQVEILKLEQSLSNARKRLGEIRRH
AYYNQDDD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2907
Mutations:
None
Discrepancy:
c1030g,g1031c,R344A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable