Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH203024.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00025163
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4026nts
Open reading frame: 1 to 4026

ATGAGCTTATCACCACACGTAGAAAATGCTTCCATTCCCAAGGGGAGTACCCCGATACCA
AAAAACAGAAACGTTAGTTCTATAGGCAAGGGCGAGTTTCTTGGATCTTCGTCAAGCAAC
AATTCTTCTTTTCGAATGAATCATTACTCAAATAGTGGACAACCTTCCGTGTTAGATTCT
ATACGGCGACCTAATCTAACTCCAACTTTTTCTTACAGTAATGGGGTTTATATGCCTGAA
AGTCACAGAACTAGTTCATTCAACGATAGTTATTTGCCATATGATAAGAATCCTTACGCA
AAAACAACCGGAAGCATGAGCAATAAATCGAACATGAAAATCAAAACAAAGAAAAATGCA
ATCAACACAAATACAAGAAAGTCGTCTGGACTCATTTACACCACCAAAGTGGATAAAGAA
TTATCTAGTATAGACAAAGTGAATGATCCAAACATTAACGGTCTTGTTTGTGCAGGTAAA
ACTCATTTGGGGCTCTATAAATTCTCGCCCTCAGATAGATCTATCAAATGCGTTCATGAT
TTCATAACACCTAATAGCAACACCTCAACAAGGGGAACGACATCCCTTTTGCCCAAGCTG
AGCAAAAGAACAAGACAGAACAAATTTAGTACCATTGCGGATGTTAAGACTGGATTTAAT
AATTACAAGAACTGTATTGCAGTGTGCAACAATTCTACGGCGATATCGATATATGATCTC
AATAAGAGTTCCTCGATAGACAATCCCTTGATAACATCCTTATGCGAACACACTAGATCT
ATCAATAGTTTTGACTTCAACATGGTCGAATCAAACCTAATAATAAGTGGTGGACAAGAT
AGTTGTGTAAAAATATGGGATTTACGTTCCAATAAATCTAAAAGCTCAAATAGATCTGAC
ATTAGTATAAATACCGCATCTGACTCAATCAGAGATGTAAAATGGATGCCCGGTTACAAT
TTTGCATCTAAGAACGATCAAGGTTCATCAACGTATGGTAATTTAAAAAGCGGTTACAAA
TTTGCTTCTATTCACGATTCGGGATACTTGCTAAAGTTTGATCTTCGACAACCTGCCCAA
TACGAAAAAAAATTAAATGCTCACACAGGACCCGGTCTTTGTTTAAATTGGCATCCCAAT
CAAGAATACATTGCTACAGGTGGTAGAGACGGTAAATGCTGCCTCTGGTTTGTTGGCGAC
AATGCCAATGCCGCCGAAAATACAGTCCTCAATTACGGGAACTCTCCCTCGTTACATGCC
CCCAATACGTCCTTGAACAATAGCGGCTCGCTGGCATTTCCCAAACTAACAATTAATACG
GGCTATCCAGTCACTAAATTGAAGTTCAAGCCTGCTTATAGTAGTAATATATATAATTCA
TTACTAGGAATATCGTCAATGGGTGACGAAGCTGAAGTTCGCATCTATTCCTTGGCAAGA
AAGTACATTCCAAAACATGTTCTCCTATCAGAAACGCCTTCTTTAGGATTAGTATGGTGG
GACGAAAACTTGATCTTTAATATCGATAAGGGGACCCGTATAAATGGCTGGGACATTAAC
AAAGAACCAACAGTGCTCGAAAATTTGAGCAAAAATACAACAACTTGGAGAGATTTGGAT
GGTAACGGTTTACTTTCAGTTGACCAAGAGATAGGCTCATATGAGGTGGTAGAACCGGAG
CTTCAACCTACCTCAAGTACCACATGCAAGAAGCATCCAGGTACAATAAAAAACCCGAAG
AATGGCAATCCAGAGAACCAGGGAATAATTGGAGGGATAAAAAAAGGATTTAGCCATACT
GGATTAACAAGTTTTACGCCAGAGAGGCCGCCTACTTTAAAAGCAGGCCCAACGTTTAGC
ACAAAAAGCTTAACATTAGCATCTGGAGCATCTTCTTTTAACAGCTCTTCCGCATCATTG
ACATCCCTGACGCCACAAACTGAAAATAGAGAAGAAATCGCCATTGAACCGCCTTGTATC
ATCACTTTGGATATACCGCAGATCTTCAATAACATAAGATTAACCAAAATCGCACATTCA
AGAAAAAAGAACGTCATTTCCGAAAGTTCTTCTATGAAAAATTCTCCAGTAGAAAAGTTC
AAATATTTGGCGAGACAGCTCAAGTTTTCGTATATTCGAGAGCATAATGTTTCAGACAGT
GCAGACACTGCATATAAAAATGATATAGAGAACATTGACGTCGTTAAAAACGCAACCGAA
ACACATGGTGATAATACTACTACTACCAATAATAATGATGATGGTGATGATGATGATGAT
GATGATGATGATGACAAAATAATCGAAAGTCACCTTTTGAAAAAATACAATTTTCCAGAA
AATAATACATGGGCTACCTTAATGAATGAAAAAGTAAATAACAAAAAATCCAAAAGAAAT
TCCAGTAGTTCGAGGGAGTTTGACGAGAAGGATGTCAGATCAAGTATATCTTCCATTTCT
GCAAGTAGGCAAAGTCATGACAGAGCTAGGAAGATCGACAAGAACGTGGAAGCAGAACTG
CAAGAAAAAATACAAACACTGGTAGATTTGATATCTATTGCAACGCATAACGCATCTGTT
TATCTATCCATAGATGATCTAACCAACTTCAAAATCTGGATCTTGATAAGAGATTCATTG
TTATGGGACTTGAAGTGGATGACCTCCTCCCAAATTTCATCAGATAACGCATCTAATATG
GATGCCAATGAAAGCTCCGATTTCGAAGCTGGAGAAAATCTGAAAACAGGAAAAGAGTTC
CCAGAGGAGGATGGTGCAGGGACAAGTGGGGCTGAGTCGTTAGTTGAAGAAAGGCCGCAG
GCATTTCGTGCTAACTCTGATGAACCAAGTGATGCCGAAAAGAAACCTGTTTCTAAGCTC
AAAGAGCAGCTCAAAAACACGGAAATAATCCCCTATGCGCAGCCAAATGAGGATTCAGAT
GAAGTTTTAACTAAGCTAAAAGAGCTACAGAACCAACGCTTGGAGTCAAGGACTAAAATG
GGAGAAACAGTGAGTGACGATGTGATTATAGAGGAAGACGAACATGAACATCAAGAAGAA
GAACAGCCTCATGACTCACCGACTAAGTCAGCACAATTTCACGCGTCTCCTATCGCGAAA
AGTATACCAATATTGCAGAAACGTGAGCACCGCAAGTCATTCATAGATACTTTCATGTTG
CATTCTCCCAACGGTTACAATGGAGATACGGACATAGGTAACGAAGATGACAATATATCT
CCTAGGTTTACTTACAACAGCGTAAGCCCACGCAGTAAGGTTTCGTCGCTACAAAGCTAT
GCCACAACAACCTCGCAACTAGAAACTTTTAAAAAGCTTTCTTCCCATACAGCACCAATA
ATCGGATCGCCCAGACATGCGCCATCACGGCCAGATAGCATTGGTAGGGAACAACTGTCT
TCTTCATTGACGAAAAAGCTTGCCAAATGCAAGAAAATTATCGCTGACCCACCCTGGGAC
ACGAAAAAACTCATCAAACAACTCTACAATCAAGCTACTGAGACTGGGAACGTCGTATTG
ACGGTGAATATTTTATTTCTTTTCCAAACGATATACCAAATAACAGAAATCGACATTGCA
AAGGACGCAATCGCTCACTTCTTGTTATTATTACATCGATACGAACTCTTCGGCATTGCT
GCAGACGTCTTAAAATACTGCCCATTCGAAGATATCATGGGTTCTGAGGGTGACCAATCT
TCCATTCGGTTGTTTTGCGAACGCTGTGGTGAGTTAATCACTAACGAAAGTTCTAAAGAA
AAGCTCAGAGCGGAAGCTCAGCAGACGGGCAATAAGAAGATCATGGACAAGTTTGGATAC
TGGTATTGTGACTCTTGTAAGAAGAAGAATACATCTTGTGTTCTATGTGAAAGACCATTA
AAGAAACTGACCATGGTCATCCTCCCCTGTGGACACGAAGGTCACTTCCAGTGCATACAA
GAATGGTTTCTCGATGAGAATGAACAAGAATGTCCCGGCGGTTGCCCCGGTGTTGCATTC
ATCTAG

Protein sequence predicted based on nucleotide sequence

MSLSPHVENASIPKGSTPIPKNRNVSSIGKGEFLGSSSSNNSSFRMNHYSNSGQPSVLDS
IRRPNLTPTFSYSNGVYMPESHRTSSFNDSYLPYDKNPYAKTTGSMSNKSNMKIKTKKNA
INTNTRKSSGLIYTTKVDKELSSIDKVNDPNINGLVCAGKTHLGLYKFSPSDRSIKCVHD
FITPNSNTSTRGTTSLLPKLSKRTRQNKFSTIADVKTGFNNYKNCIAVCNNSTAISIYDL
NKSSSIDNPLITSLCEHTRSINSFDFNMVESNLIISGGQDSCVKIWDLRSNKSKSSNRSD
ISINTASDSIRDVKWMPGYNFASKNDQGSSTYGNLKSGYKFASIHDSGYLLKFDLRQPAQ
YEKKLNAHTGPGLCLNWHPNQEYIATGGRDGKCCLWFVGDNANAAENTVLNYGNSPSLHA
PNTSLNNSGSLAFPKLTINTGYPVTKLKFKPAYSSNIYNSLLGISSMGDEAEVRIYSLAR
KYIPKHVLLSETPSLGLVWWDENLIFNIDKGTRINGWDINKEPTVLENLSKNTTTWRDLD
GNGLLSVDQEIGSYEVVEPELQPTSSTTCKKHPGTIKNPKNGNPENQGIIGGIKKGFSHT
GLTSFTPERPPTLKAGPTFSTKSLTLASGASSFNSSSASLTSLTPQTENREEIAIEPPCI
ITLDIPQIFNNIRLTKIAHSRKKNVISESSSMKNSPVEKFKYLARQLKFSYIREHNVSDS
ADTAYKNDIENIDVVKNATETHGDNTTTTNNNDDGDDDDDDDDDDKIIESHLLKKYNFPE
NNTWATLMNEKVNNKKSKRNSSSSREFDEKDVRSSISSISASRQSHDRARKIDKNVEAEL
QEKIQTLVDLISIATHNASVYLSIDDLTNFKIWILIRDSLLWDLKWMTSSQISSDNASNM
DANESSDFEAGENLKTGKEFPEEDGAGTSGAESLVEERPQAFRANSDEPSDAEKKPVSKL
KEQLKNTEIIPYAQPNEDSDEVLTKLKELQNQRLESRTKMGETVSDDVIIEEDEHEHQEE
EQPHDSPTKSAQFHASPIAKSIPILQKREHRKSFIDTFMLHSPNGYNGDTDIGNEDDNIS
PRFTYNSVSPRSKVSSLQSYATTTSQLETFKKLSSHTAPIIGSPRHAPSRPDSIGREQLS
SSLTKKLAKCKKIIADPPWDTKKLIKQLYNQATETGNVVLTVNILFLFQTIYQITEIDIA
KDAIAHFLLLLHRYELFGIAADVLKYCPFEDIMGSEGDQSSIRLFCERCGELITNESSKE
KLRAEAQQTGNKKIMDKFGYWYCDSCKKKNTSCVLCERPLKKLTMVILPCGHEGHFQCIQ
EWFLDENEQECPGGCPGVAFI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4026
Mutations:
None
Discrepancy:
c1178g,S393C; g1643a,G548D;
 t3366a
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable