Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH207143.01X
Keyword:
None
Species:
Yersinia pseudotuberculosis
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YsCD00025433
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2136nts
Open reading frame: 1 to 2136

ATGAGCGTAACAGTTTCCGGCATTATGATTAACCCGGTCGGCGAGCCAGTAGTCAATGCA
CAGATCACCCTCACAGCGGTTACCAATAGCCTTACTGTGCTCAATGCCTTTTCGGCCACA
GTCAGAACGGACGGGGTGGGTACATATCGCATCCAGTTAGAAGAGGGCAGCTACTCCATT
ACAGTGGCTGCGAATGGCCGTAGTTTTGTTTATGGCGCGGTCACGCTGGATAACACCACA
GGCCCCAGCACACTCAATCAATTGCTGAAGCAGCAGATCATGGAGTCGGAACTCACACCT
GATGTGATCCTGTACTTCCGCCAGATTCAACAGCAGGTCGCAAATGATCTTGCAACAATA
AAAGTTTTAGAAATCAGTGCTACAGATGCGGCAGAGAGCGCAGGTCATTCACGCGATGAG
GCTATGTTGTATGCAAAGGATTTGTCTGAGGCGCTTGCAACAGCCAAGGGGTATCGCGAC
CAAGCGGGCATAAGTGCGGATGCATCTGCACTCTCTCAGCAGGAAGCGGCGATAAGTGAA
ACGAGTGCTAAAGCCAGTGCCGATTCAGCATTACTGTCAGAGCAAAATGCGCTTTCATAT
CGCGACTCAGCCCAGTCAGCAGCGGCCACAGCAGCCGATGATGCGTCAACACTTGCAGCC
GAGCGGACAGCAGAAAAAATTAAGTTACAAGTTAAAACTGATGCGGATCGTGCCGAAGCG
GCACGTATAGCAAGTGAACAGATAAAATCCTCGGTAGATGACACAGCTCAGACAGTTGCT
CAACAGCATGGCGAAACAACACAAGCAGCTATCGCCGCACGAGATAGTGAAGTCAAGGCA
ACAACCGCGGCAAACAGTGCTGTTCAATCGGAAGCGTTAGCGGCAATCAGTGCGGAAACA
GCACGACAGAACGCAGGAATATCTACAGTAGATAAAAATGCAGCAAAAGGTTTTAGAGAT
GAGGCTGAGGGTTTTGCTCAGCAGGCGCATGCTTCAGCAGAAAGCGTCGGCGATGTAATG
CCAAAAACTGGTGGTGCGTTCACAGGCCCAGTAGAGTTGGCAGGTGATGCCACAGAGCCA
TTAGAGCCTGTCACATTTCAACAGTTTGAAAGGACCGGTGGCGAATTTCTATTAAGTGTG
AAGTGGCATATGAGCCGAAATTATATTCCTGCGGGCTGGGCCCCAGCGGACGGTCAATTA
CTATCACGCAATCTATTTCCATTCGCTTTAGCTGAAATATTAAGCGCGAAATATCCGATC
GTTGCGGATGACTCGTGGTTATTTTACAAAGACCAGCGATCTAGTTTTTCGGTCGGTGAC
GGAAGTACAACATTCAGAATTCCGGATTTGAACGGCAAGTCCCATGACAGCATGGGAAGA
GTGTTTTTGGGGGGGGACGGTAAGAACTCCCTCGGAGAAATGGGGAGAATCCAAGGCGAC
GCGAGTAGAAGAATTACTGGGACGTTCGGTGGAATTGGCGGGCAACTTAATGTTTCGTAT
GGTCTGGTTATAGGTGAGACTGCCGGAGCTTTTACAAGAACTGGCGTGGCAACTGGAAGG
CCGGTTCCATCAAATATTGGGGAGCCCGCGCTAGGTGAGCTTGGTGTTAGTTTTGACTCT
GCATTAGTAAACCCCACTGCAATCGAAAATCGCCCCATCAACGCCACGGGCTGCTACATA
ATAAAATTGGCAGGGTCGGCTCTCAATGAAGGGCAAATTAACGCGCTCGAATTAGCAACG
CAGATAACACAACTTGCATCGCGCACTACAACTTTAGAGGCTGACGCGTTTACTGCTAAT
AAAATAGCAAATACGCCATGGATTCCGCTAACACTTCAAAACGGCTGGTTACCCCTTCAG
GGTTATCACAATGCAATCTATCGGAAAATAAATGGAGTCGTATACGTTGAAGGAGTTATC
ACCGCAGGTGCATGGGGAGAAAGCGTGGTTATTGCAAATCTACCCGCTGGATATAGGCCA
TCAGCTGATCAAGTGAGTGTCATGCCAATTTCAGGTAGCACGCAGGGTGGCATTACAGCG
CAATCGAGAATTGCGTTGTGGACGGATGGGGCGTTAAGAATTTATGGAGTAACAGGAAAC
GGAGATATTGGAATAAAGTCACACTGGGTGATTTAG

Protein sequence predicted based on nucleotide sequence

MSVTVSGIMINPVGEPVVNAQITLTAVTNSLTVLNAFSATVRTDGVGTYRIQLEEGSYSI
TVAANGRSFVYGAVTLDNTTGPSTLNQLLKQQIMESELTPDVILYFRQIQQQVANDLATI
KVLEISATDAAESAGHSRDEAMLYAKDLSEALATAKGYRDQAGISADASALSQQEAAISE
TSAKASADSALLSEQNALSYRDSAQSAAATAADDASTLAAERTAEKIKLQVKTDADRAEA
ARIASEQIKSSVDDTAQTVAQQHGETTQAAIAARDSEVKATTAANSAVQSEALAAISAET
ARQNAGISTVDKNAAKGFRDEAEGFAQQAHASAESVGDVMPKTGGAFTGPVELAGDATEP
LEPVTFQQFERTGGEFLLSVKWHMSRNYIPAGWAPADGQLLSRNLFPFALAEILSAKYPI
VADDSWLFYKDQRSSFSVGDGSTTFRIPDLNGKSHDSMGRVFLGGDGKNSLGEMGRIQGD
ASRRITGTFGGIGGQLNVSYGLVIGETAGAFTRTGVATGRPVPSNIGEPALGELGVSFDS
ALVNPTAIENRPINATGCYIIKLAGSALNEGQINALELATQITQLASRTTTLEADAFTAN
KIANTPWIPLTLQNGWLPLQGYHNAIYRKINGVVYVEGVITAGAWGESVVIANLPAGYRP
SADQVSVMPISGSTQGGITAQSRIALWTDGALRIYGVTGNGDIGIKSHWVI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2136
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable