chiA-1 (V. cholerae O1 biovar El Tor str. N16961 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH200936.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
VcCD00025632
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2541nts
Open reading frame: 1 to 2541

ATGAAGCGCTATTGTTTAGCCGCCGTGATTACCGCCAGTTTGGGGGTAAGTTACTCAGCA
CAAGCCTATAACTGTGCCGGAGTGCCAGTGTGGGACAGCAGTACAGTGTATGTTGGCAGT
GATAAAGTTCAAAAAACCAACACCGCTTACCAAGCGCGTTACTGGACTCAGGGTAACGAT
CCGGTAACCCATTCTGGTCAATGGGACGCATGGCAAATTCTTGGACAGTGTGACGGAGGG
GCGAATAACCCACCTCAAGTGTCCATTCAATCACCACTCAATAACGCCAAGATCCCACAA
GGCTCCGTCGTCGGATTACAAGCCAATGCCTCAGATAGTGATGGCAGCATTACTCAGGTG
GAGTTTTTAGTCGGCACACAGCGTATCGCGATTGACCAACAAGCCCCATACCAAGTGGAT
TGGACGGCAACGCTAGGAGCCACTTCAGTGACCGCGATTGCGACCGATAACCAAGGTGCA
ACCACCAGCAGCACCGTAAATATCAGCGTAACGCCTACTGGTAACCCAGTTCCGCCAACT
GTGACGTTGACTAGCCCAACGGGCAGTGAACAGCTAACCGTGGGCGATGTTCTAGCCGTG
GCCGCAAATGCCACTGACAGTGATGGCACTGTCAACGCTGTTGAATTTTATGTTGATGGC
CAACTTGTTGTGATCGATAGCTCTGAGCCTTATCAGTTTAATTGGAATGCCGCTGTCGGC
AGCCATACCTTTAAAGCCAAAGCGATTGATAACGACAACCTGTCTACCCTGAGCCAAGAA
GTGACTTTAACGGTCGGCAGTGGCTCCAATGCAGGTTGCGCGGGTTTACCTGTGTATTCG
GTGGGAACCGCGTATTCGGCAGGGCAGTTGGTACAAAATAAGAACCAAAAATATCGCTGT
GATATCGCCGGTTGGTGTTCTTCCAGCTCCGGTTGGGCGTATGAGCCAGGGGTTGGAAGT
TATTGGAAAGAAGCATGGAGTGGTTTAGGCGCTTGTTCAACGCCACCTGTCGTGACATTA
ACCAACCCAACGGCGAACCAAGTGATTCTTGCTGGCTCAACCGTCAGTGTGGCGGCGCAA
GCCAGTGATGCCGATGGCTCGGTCATGCAAGTGGAGTTTTTTGCGGGCAACAACAGCTTA
GGCGTGGTGACTCAAGCACCTTACGCGGTTAACTGGCTAGCCACTACTACGGGAAATCAA
ACCCTGAAAGCAGTCGCAACCGACAACGACAGTAACACCAGTGAAAGCGCAGTCAGCGTG
ACGGTGAGCGATCAAGATCTGGTGGTTTCACTGACTTCACCGACATCCGGCCAAACCGTT
GGTCTAGGTAAACCGGTCAATATTGCCGCCGATGCTACGTCACTAACCAATAATGTGGCG
AAAGTGGAGTTTGTGGTCAACGGCGCGGTAGTTGCAACGGATACAACAGAGCCTTTTGCT
TACAGTTGGACGCCAAGTGCGATTGGTAACTATACCGTTGCCGCGAAAGCGACTGACGCA
GCAGGAACGAGCGTGACCTCTTCTGCTGCCGCAATCAGCGTGGTAGAGCAAGCGCAGAAG
AAACATCGCTTGATTGGTTACTGGCATAACTTCGTCAATGGTGCGGGTTGCCCAATTCGT
TTGGCGGATATGTCACAAGCGTGGGATGTGATTGATATTGCCTTTGCAGAAAACGATCGC
AACAGTACCGGAACGGTACATTTCAACCTGTATGCCGGTGATATTTACAGCAGTTGTCCT
GCGCTCGATCCTGCGCAATTCAAGCAAGATATGAAAGCACTGCAAGCGAAAGGTAAAGTA
TTTGTCCTTTCCTTAGGTGGCGCTGAAGGCACTATTACCCTTAACACTGATCAAGATGAA
GCCAACTTCGTGAGCAGCCTGACTGCGTTGATCAAAGAATGGGGCTTTGATGGGTTGGAT
GTGGATCTCGAAAGTGGTTCAAACCTTGTTCATGGCTCACAAATTCAAGCGCGCTTAGGC
CGAGCATTGAAGCAAATCGAGAAAAACATCGGTGGTGATATGTTCCTCACTATGGCGCCT
GAACACCCTTATGTGCAAGGTGGTATGGTTGCCTACAGTGGAATTTGGGGCGCTTACATT
CCGGTGATTAACGAAGTGCGCGATACGCTTGATATTCTGCATGTTCAGCTCTACAACAAC
GGTGGTTTACCTAACCCATACACACCGAGCGCGGCACCAGAAGGCTCTGTGGATATGATG
GTCGCCCAATCAAAAATGTTGATTGAAGGCTTCACTTTAGCCAACGGGACACGCTTTGAA
CCATTGCGTGATGACCAAGTAGCCATTGGTTTGCCATCAGGTCCAAGTTCGGCAAATTCG
GGTCAAGCTCCAACCCAAAATATCCTTGATGCGTTGGATTGTTTAACCAAAGGAACCCGC
TGCGGCACCATCAAACCAGCCTTTGCCTACCCCAATTATGCAGGCGTGATGACCTGGTCA
ATCAACTGGGATAAGCACGACGGCTTTAATTTTTCCAAACCGGTTGGGGATAAACTTAGC
CAGATGAATAATGCTCAGTTG

Protein sequence predicted based on nucleotide sequence

MKRYCLAAVITASLGVSYSAQAYNCAGVPVWDSSTVYVGSDKVQKTNTAYQARYWTQGND
PVTHSGQWDAWQILGQCDGGANNPPQVSIQSPLNNAKIPQGSVVGLQANASDSDGSITQV
EFLVGTQRIAIDQQAPYQVDWTATLGATSVTAIATDNQGATTSSTVNISVTPTGNPVPPT
VTLTSPTGSEQLTVGDVLAVAANATDSDGTVNAVEFYVDGQLVVIDSSEPYQFNWNAAVG
SHTFKAKAIDNDNLSTLSQEVTLTVGSGSNAGCAGLPVYSVGTAYSAGQLVQNKNQKYRC
DIAGWCSSSSGWAYEPGVGSYWKEAWSGLGACSTPPVVTLTNPTANQVILAGSTVSVAAQ
ASDADGSVMQVEFFAGNNSLGVVTQAPYAVNWLATTTGNQTLKAVATDNDSNTSESAVSV
TVSDQDLVVSLTSPTSGQTVGLGKPVNIAADATSLTNNVAKVEFVVNGAVVATDTTEPFA
YSWTPSAIGNYTVAAKATDAAGTSVTSSAAAISVVEQAQKKHRLIGYWHNFVNGAGCPIR
LADMSQAWDVIDIAFAENDRNSTGTVHFNLYAGDIYSSCPALDPAQFKQDMKALQAKGKV
FVLSLGGAEGTITLNTDQDEANFVSSLTALIKEWGFDGLDVDLESGSNLVHGSQIQARLG
RALKQIEKNIGGDMFLTMAPEHPYVQGGMVAYSGIWGAYIPVINEVRDTLDILHVQLYNN
GGLPNPYTPSAAPEGSVDMMVAQSKMLIEGFTLANGTRFEPLRDDQVAIGLPSGPSSANS
GQAPTQNILDALDCLTKGTRCGTIKPAFAYPNYAGVMTWSINWDKHDGFNFSKPVGDKLS
QMNNAQL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2541
Mutations:
None
Discrepancy:
g807c
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable