Gene:
Gene Symbol:
TM1761
Gene Name:
excinuclease ABC subunit B
Original Clone ID:
7955
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00282215
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1992nts
Open reading frame: 37 to 2028
ATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTCAAGCTGGTGAGTGAATTT
GAGCCAACGGGTGACCAGCCACAGGCCATAGAAAAACTTGTAGAAGGTTTGAACAGGGGA
ATGAGATTTCAAACGCTCCTCGGTGTTACGGGGAGCGGAAAGACTTTCACCATGGCCAAC
GTGATAGCGCGTGTGAACAGACCCGCCCTTGTGATCTCACCGAACAAGACGCTTGCCGCC
CAGCTTTATCAGGAGTTCAAAACGTTCTTCCCGGAAAATCGAGTGGAGTTCTTCATAAGT
TACTACGATTACTATCAACCGGAGGCCTATATTCCAACGAAGGATCTTTACATAGAGAAG
AACGCTGACATCAACGATGTGATCGTGCGAATGAGAATGTCCACGTTGAAATCTGTGAGA
ACTCGTCGCGATGTGGTGGTAGTTGCGAGTGTTTCGTGTATTTACGCCACGGGAGACCCG
AACGATTTCGATAGGATGAACATAAATCTCGCTGTTGGAGATAGAATAGACGTTCTGGAA
CTCGCGGAAAGACTGGCAAGGATAGGCTATCAGAGAACGGAGGATGTGTCTCTTTCTGGA
TGTTTCAGGCTGAAGGGAGACACGGTGGAGATCTACCCGACGTATCAGGATGAAGGGATC
AGGATCGAATTCTTCGGAGACGAAGTGGACTCGATAACGTTGATAGACAGATTCAATCGC
ACCACACTGGAGCACCTTGACAAGATCATCATCTATCCCGCCGTTGAATTTATCACAACG
GAGGAGAAACTCAAAAGAGCCGTCGAATCAATAAGGGAGGAGCTTAACGAAAGACTCTCA
GAGCTCAAAAAACAGGGAAAGATCCTCGAGTACGAACGCTTGAAGCAGAGAACTCTGAAC
GACATAGAGCTGCTGGAGACCATGGGATACTGTCCTGGAATAGAGAACTACTCCAGGCAC
TTCGATGGAAGAAAGCCGGGAGAGCCTCCGTACACTCTTCTCGATTATTTCGATAAAGAT
TTCATCGTTTTCATAGACGAGTCACACATCACGGTTCCTCAACTCAGGGCCATGTACAAC
GGAGACAGATCCAGAAAGAAAAATCTGGTCGAATACGGTTTTCGTCTGCCTTCCGCTTAC
GACAACAGACCGCTGACTTTCGAAGAATTTTTGAAAAAAACAGGCCAGATCATCTTCGTT
TCCGCTACGCCGGGAGACTTCGAACTCTCGATCTCTGAACAGGTCGTCGAGCAGATCATT
AGACCCACAGGACTCGTGGATCCAGAGGTGGAGGTACGTCCCACCGCGGGGCAAGTGGAT
GATCTTGTGAACGAGATCGTGAAAGTGAAAGAAAGAGGGGAGAGGGCCCTCGTTACGGTG
CTCACCAAAAAAACGGCAGAGCTTTTGAGTGAACATCTGACGGAACTTGGAATAAGATCG
CTCTATCTTCACTCCGAACTGGACGCCATAGAAAGGGTTGAGGTCCTGAAGAAACTGAGG
CGAGGAGACGTAGACGTGGTCGTTGGTGTGAACCTTCTGAGGGAGGGTCTCGATCTTCCC
GAGGTGTCACTGGTAGCGATCATGGACGCGGACGTGGAAGGCTTCCTGCGATCTGAAACA
ACGCTCATCCAGATTATAGGGAGAACCGCAAGGAACGTCAACGGGAAGGTCATCATGTAC
GCCGACAGGATAACAAACGCGATGAAGAGAGCCATCGAAGAAACGAACAGAAGAAGGAGA
ATTCAGCTCGAGTACAATAGAAAACACGGTATCACTCCACGTTCCGTGATAAAGCCTCTG
GAGATAGAAGTATTCGAACAGTTCATGGTGAAAGAAGAGCCTGAGAGGTACGGAGACACT
GTGAAAAACATCTTTGAGATGAAGAAAACGCTCTCCCCAGAGGAGTACATGGCCGTTCTC
GAAGAGGAAATGTATCGGGCTGCAAGTGAGCTCAGGTACGAAGATGCAGCGGCCTTGAGG
GATGAGCTGTTCAGAATCAGGGAAGAGATAAAAAAGAAAAAGGGGTAA
Protein sequence predicted based on nucleotide sequence
VFKLVSEFEPTGDQPQAIEKLVEGLNRGMRFQTLLGVTGSGKTFTMANVIARVNRPALVI SPNKTLAAQLYQEFKTFFPENRVEFFISYYDYYQPEAYIPTKDLYIEKNADINDVIVRMR MSTLKSVRTRRDVVVVASVSCIYATGDPNDFDRMNINLAVGDRIDVLELAERLARIGYQR TEDVSLSGCFRLKGDTVEIYPTYQDEGIRIEFFGDEVDSITLIDRFNRTTLEHLDKIIIY PAVEFITTEEKLKRAVESIREELNERLSELKKQGKILEYERLKQRTLNDIELLETMGYCP GIENYSRHFDGRKPGEPPYTLLDYFDKDFIVFIDESHITVPQLRAMYNGDRSRKKNLVEY GFRLPSAYDNRPLTFEEFLKKTGQIIFVSATPGDFELSISEQVVEQIIRPTGLVDPEVEV RPTAGQVDDLVNEIVKVKERGERALVTVLTKKTAELLSEHLTELGIRSLYLHSELDAIER VEVLKKLRRGDVDVVVGVNLLREGLDLPEVSLVAIMDADVEGFLRSETTLIQIIGRTARN VNGKVIMYADRITNAMKRAIEETNRRRRIQLEYNRKHGITPRSVIKPLEIEVFEQFMVKE EPERYGDTVKNIFEMKKTLSPEEYMAVLEEEMYRAASELRYEDAAALRDELFRIREEIKK KKG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1995
Mutations:
None
Discrepancy:
del@1990,3;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 283615
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
