Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
15321
Keyword:
None
Species:
Deinococcus radiodurans
Type:
cDNA
Vector Name:
pMH4
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
DrCD00282835
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 297nts
Open reading frame: 37 to 333

ATGGGATCTGATAAAATTCATCATCATCATCATCACATGCGAGACGGGCTTTCTCCATCC
CGACCCCCTGTTTTGCCGGGGACGGCGCCCAGCCGGCGCTCGCCCCCGCCCCGAAGGACC
CCACTGATGCCCACCTACCTCTACAAAGACCTGAACACCGGCGAAATCTACGAACTCGTG
CAGCACATGCGCGACGACCCCTACACCGAGCACCCCGAAACCGGCGCGCCGGTCAAACGG
GTGCTGTCGCGCCCGGCCATCGCCTTTAAAGGCAGCGGCTTTTACGTCAACGACTCGCGC
AAGTCGGGCGGCGAGGGGGGCGGCGGCGAGTGACGCGACTTAATTAACTCTAGATAACTG

Protein sequence predicted based on nucleotide sequence

MRDGLSPSRPPVLPGTAPSRRSPPPRRTPLMPTYLYKDLNTGEIYELVQHMRDDPYTEHP
ETGAPVKRVLSRPAIAFKGSGFYVNDSRKSGGEGGGGE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 297
Mutations:
None
Discrepancy:
g1a,V1M;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 357205
Recommended expression in:
Not Applicable