Q2ZV16_SHEPU (S. putrefaciens )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
10412k2BCt7p1
Keyword:
intended mutation
Species:
Shewanella putrefaciens
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SpCD00285527
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 387nts
Open reading frame: 51 to 437

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAAATTTTTG
AGTTCGTGCCGCCGACCGATAAAGAGATCGAGGAGGCGCAGCTGAAACTGGGCTTTAAAT
TCAGCCCTGAATATATTGAATTTATCAAATCTGGTTACGACCTGGGGGATGCCCCGATTG
AAGCACTGGAAATCAGTAATCCACCTTCACATGCTGACATTTTCGAGACGCTGGCGAACG
CCCGTAAATATTTTCAAATGCCGTCCGAAATGTGCCCGATCTGTGAAGATAATTCGGACT
ACTATTGCCTGAACCAGAAAGGAGAGGTTGTATTCTGGAGCCACAATGGCACTACATCTG
AAAAATGGGCAAACGTAACCGTGTGGCGCAATCAAATGGCTACGGAGGTTGAAGGGCATC
ATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG
CACCAC

Protein sequence predicted based on nucleotide sequence

KIFEFVPPTDKEIEEAQLKLGFKFSPEYIEFIKSGYDLGDAPIEALEISNPPSHADIFET
LANARKYFQMPSEMCPICEDNSDYYCLNQKGEVVFWSHNGTTSEKWANVTVWRNQMATEV
EGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 387
Mutations:
L71M; L75M;

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-10412k
Recommended expression in:
Not Applicable