Gene:
Gene Symbol:
PTPN3
Gene Name:
protein tyrosine phosphatase, non-receptor type 3
Original Clone ID:
8603a1ZBt2
Keyword:
intended mutation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCR4zeroBLUNT-TOPO
Format:
FUSION
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"Invitrogen Zero Blunt, TOPO"
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
HsCD00285675
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
ATTCGCCCTTCGCTCATTTGCTGACTTCAAGTCTGAAGATGAACTGAACCAGCTTTTCCC
CGAAGCCATTTTCCCCATGTATCCGGAGGGTGGGGACACTTTGGAGGGATCCATGGCACA
GCTAAAGAAGGGCCTCGAAAGCGGGACGGTGCTGATCCAGTTTGAGCAACTCTACAGAAA
AAAGCCAGGTTTGGCCATCACGTTTGCAAAGCTGCCTCAAAATTTGGACAAAAACCGATA
TAAAGATGTGCTGCCTTATGACACCACCCGGGTATTATTGCAGGGAAATGAAGATTATAT
TAATGCAAGTTACGTGAACATGGAAATTCCTGCTGCTAACCTTGTGAACAAGTACATCGC
CACTCAGGGGCCCCTGCCGCATACCTGTGCACAGTTTTGGCAGGTTGTCTGGGATCAGAA
GTTGTCACTCATTGTCATGTTGACGACTCTCACAGAACGAGGGCGGACCAAATGTCACCA
GTACTGGCCAGATCCCCCCGACGTCATGAACCACGGCGGCTTTCACATCCAGTGTCAGTC
AGAGGACTGCACCATCGCCTATGTGTCCCGAGAAATGCTGGTCACAAACACCCAGACCGG
GGAAGAACACACAGTGACACATCTCCAGTACGTCGCATGGCCTGACCACGGTGTGCCCGA
TGACTCCTCCGACTTTCTGGAATTTGTAAACTATGTGAGGTCTCTGAGAGTGGACAGCGA
GCCCGTCCTAGTTCACTGCAGTGCTGGAATAGGTCGAACCGGTGTGTTGGTCACTATGGA
AACAGCCATGTGCCTAACTGAGAGGAACCTGCCCATTTACCCACTGGATATTGTCCGAAA
AATGCGAGACCAGCGCGCCATGATGGTGCAGACATCAAGCCAGTACAAGTTTGTGTGTGA
AGCGATTCTTCGTGTGTATGAAGAAGGTTTAGTCCAAATGCTGGATCCTAGTAAGGGGCG
AATTCGT
Protein sequence predicted based on nucleotide sequence
RSFADFKSEDELNQLFPEAIFPMYPEGGDTLEGSMAQLKKGLESGTVLIQFEQLYRKKPG LAITFAKLPQNLDKNRYKDVLPYDTTRVLLQGNEDYINASYVNMEIPAANLVNKYIATQG PLPHTCAQFWQVVWDQKLSLIVMLTTLTERGRTKCHQYWPDPPDVMNHGGFHIQCQSEDC TIAYVSREMLVTNTQTGEEHTVTHLQYVAWPDHGVPDDSSDFLEFVNYVRSLRVDSEPVL VHCSAGIGRTGVLVTMETAMCLTERNLPIYPLDIVRKMRDQRAMMVQTSSQYKFVCEAIL RVYEEGLVQMLDPS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
942
Mutations:
C623Y; L838V;
Discrepancy:
g940a,a942t,G314S;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50 ug/mL ampicillin
Growth Condition:
Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)
Comments:
None
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in NYSGXRC-8603a
Recommended expression in:
Not Applicable
Vector Name:
pCR4zeroBLUNT-TOPO
Synonyms:
pCR-4, pCR-IV, pCR4, pCR4-TOPO
Description:
TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.
Comments:
Empty vector is a product available from Invitrogen.
Size (bp):
3956
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin of replication
3161
3834
primer site
M13 reverse
M13 reverse priming site
205
221
primer site
T3
T3 priming site
243
262
primer site
M13 forward
M13 forward priming site
355
370
primer site
T7
T7 priming site
328
347
promoter
lac
lac promoter region
2
216
selectable marker
AmpR
Ampicillin resistance gene
2203
3063
selectable marker
KanR
Kanamycin resistance gene
1159
1953
