DNASU Plasmid | C11orf8 (H. sapiens ) in pCR4zeroBLUNT-TOPO (TOPO cloning vector)

Explanation of Terms

Gene:

Gene Symbol: MPPED2

Gene Name: metallophosphoesterase domain containing 2

Original Clone ID:

8639a1ZBt2

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCR4zeroBLUNT-TOPO

Format:

FUSION

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

"Invitrogen Zero Blunt, TOPO"

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00285694

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 879nts

Open reading frame: 10 to 888

Reference Sequence Alignment

ATCGCCCTTGCACATGGGATTCCTTCTCAAGGCAAAGTTACCATAACGGTGGATGAGTAC
AGCTCAAACCCCACCCAGGCATTCACGCACTACAACATCAACCAGAGCAGATTCCAGCCT
CCACATGTACATATGGTCGACCCCATCCCATATGACACTCCAAAACCAGCGGGCCACACG
CGGTTTGTCTGCATCTCAGACACACACTCCAGAACAGATGGTATCCAGATGCCTTATGGG
GACATCCTTCTCCACACAGGCGATTTCACCGAGCTGGGACTGCCCTCAGAGGTTAAGAAG
TTTAATGACTGGTTAGGAAACCTGCCATATGAATATAAAATAGTGATTGCTGGGAATCAT
GAACTGACATTTGATAAGGAATTCATGGCAGACCTTGTTAAACAGGACTACTACCGTTTC
CCCTCTGTGTCCAAATTGAAACCAGAGGACTTTGACAATGTTCAGTCCCTCCTGACAAAC
AGTATTTACTTACAAGATTCGGAGGTAACAGTGAAGGGATTCAGGATATACGGTGCACCT
TGGACCCCGTGGTTTAATGGATGGGGCTTTAACCTACCCAGAGGTCAGTCTCTGCTGGAC
AAGTGGAACCTCATCCCTGAGGGCATTGACATACTCATGACACATGGACCTCCTCTAGGT
TTTCGAGACTGGGTTCCAAAGGAGCTTCAAAGAGTGGGCTGTGTGGAGCTGTTAAACACG
GTTCAGAGGCGAGTCCGGCCCAAGCTCCATGTGTTTGGTGGAATCCATGAAGGTTATGGC
ATCATGACCGACGGTTACACAACGTACATCAATGCCTCGACGTGTACAGTCAGCTTTCAA
CCGACCAACCCTCCAATTATATTTGACCTTCCAAACCCACAGGGTTCCAAGGGCGAATTC
GTTTAAACCTGCAGGACT

Protein sequence predicted based on nucleotide sequence

AHGIPSQGKVTITVDEYSSNPTQAFTHYNINQSRFQPPHVHMVDPIPYDTPKPAGHTRFV
CISDTHSRTDGIQMPYGDILLHTGDFTELGLPSEVKKFNDWLGNLPYEYKIVIAGNHELT
FDKEFMADLVKQDYYRFPSVSKLKPEDFDNVQSLLTNSIYLQDSEVTVKGFRIYGAPWTP
WFNGWGFNLPRGQSLLDKWNLIPEGIDILMTHGPPLGFRDWVPKELQRVGCVELLNTVQR
RVRPKLHVFGGIHEGYGIMTDGYTTYINASTCTVSFQPTNPPIIFDLPNPQGS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 879

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50 ug/mL ampicillin

Growth Condition: Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in NYSGXRC-8639a

Recommended expression in:

Not Applicable

Vector Name:

pCR4zeroBLUNT-TOPO

Synonyms:

pCR-4, pCR-IV, pCR4, pCR4-TOPO

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.

Comments:

Empty vector is a product available from Invitrogen.

Size (bp):

3956

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGCGAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC bacterial origin of replication

3161

3834

primer site

M13 reverse

M13 reverse priming site

205

221

primer site

T3 priming site

243

262

primer site

M13 forward

M13 forward priming site

355

370

primer site

T7 priming site

328

347

promoter

lac

lac promoter region

216

selectable marker

AmpR

Ampicillin resistance gene

2203

3063

selectable marker

KanR

Kanamycin resistance gene

1159

1953

Gene

Gene Symbol :

MPPED2

Symbol Nomenclature :

MPPED2

SYNONYM :

239FB; C11orf8

Designation :

fetal brain protein 239|metallophosphoesterase MPPED2|metallophosphoesterase domain-containing protein 2

Full Nomenclature :

metallophosphoesterase domain containing 2

GENEID :

744

HGNC :

1180

MIM :

600911

Vega :

OTTHUMG00000166159

Target GenBank:

NP_001575

Reference Sequence Alignment

  

HsCD00285694        ATCGCCCTTGCACATGGGATTCCTTCTCAAGGCAAAGTTACCATAACGGTGGATGAGTAC
XM_005253110.1      ------ATGGCACATGGGATTCCTTCTCAAGGCAAAGTTACCATAACGGTGGATGAGTAC
XM_005253112.1      ------ATGGCACATGGGATTCCTTCTCAAGGCAAAGTTACCATAACGGTGGATGAGTAC
NM_001584.2         ------ATGGCACATGGGATTCCTTCTCAAGGCAAAGTTACCATAACGGTGGATGAGTAC
                          .* ***************************************************

HsCD00285694        AGCTCAAACCCCACCCAGGCATTCACGCACTACAACATCAACCAGAGCAGATTCCAGCCT
XM_005253110.1      AGCTCAAACCCCACCCAGGCATTCACGCACTACAACATCAACCAGAGCAGATTCCAGCCT
XM_005253112.1      AGCTCAAACCCCACCCAGGCATTCACGCACTACAACATCAACCAGAGCAGATTCCAGCCT
NM_001584.2         AGCTCAAACCCCACCCAGGCATTCACGCACTACAACATCAACCAGAGCAGATTCCAGCCT
                    ************************************************************

HsCD00285694        CCACATGTACATATGGTCGACCCCATCCCATATGACACTCCAAAACCAGCGGGCCACACG
XM_005253110.1      CCACATGTACATATGGTCGACCCCATCCCATATGACACTCCAAAACCAGCGGGCCACACG
XM_005253112.1      CCACATGTACATATGGTCGACCCCATCCCATATGACACTCCAAAACCAGCGGGCCACACG
NM_001584.2         CCACATGTACATATGGTCGACCCCATCCCATATGACACTCCAAAACCAGCGGGCCACACG
                    ************************************************************

HsCD00285694        CGGTTTGTCTGCATCTCAGACACACACTCCAGAACAGATGGTATCCAGATGCCTTATGGG
XM_005253110.1      CGGTTTGTCTGCATCTCAGACACACACTCCAGAACAGATGGTATCCAGATGCCTTATGGG
XM_005253112.1      CGGTTTGTCTGCATCTCAGACACACACTCCAGAACAGATGGTATCCAGATGCCTTATGGG
NM_001584.2         CGGTTTGTCTGCATCTCAGACACACACTCCAGAACAGATGGTATCCAGATGCCTTATGGG
                    ************************************************************

HsCD00285694        GACATCCTTCTCCACACAGGCGATTTCACCGAGCTGGGACTGCCCTCAGAGGTTAAGAAG
XM_005253110.1      GACATCCTTCTCCACACAGGCGATTTCACCGAGCTGGGACTGCCCTCAGAGGTTAAGAAG
XM_005253112.1      GACATCCTTCTCCACACAGGCGATTTCACCGAGCTGGGACTGCCCTCAGAGGTTAAGAAG
NM_001584.2         GACATCCTTCTCCACACAGGCGATTTCACCGAGCTGGGACTGCCCTCAGAGGTTAAGAAG
                    ************************************************************

HsCD00285694        TTTAATGACTGGTTAGGAAACCTGCCATATGAATATAAAATAGTGATTGCTGGGAATCAT
XM_005253110.1      TTTAATGACTGGTTAGGAAACCTGCCATATGAATATAAAATAGTGATTGCTGGGAATCAT
XM_005253112.1      TTTAATGACTGGTTAGGAAACCTGCCATATGAATATAAAATAGTGATTGCTGGGAATCAT
NM_001584.2         TTTAATGACTGGTTAGGAAACCTGCCATATGAATATAAAATAGTGATTGCTGGGAATCAT
                    ************************************************************

HsCD00285694        GAACTGACATTTGATAAGGAATTCATGGCAGACCTTGTTAAACAGGACTACTACCGTTTC
XM_005253110.1      GAACTGACATTTGATAAGGAATTCATGGCAGACCTTGTTAAACAGGACTACTACCGTTTC
XM_005253112.1      GAACTGACATTTGATAAGGAATTCATGGCAGACCTTGTTAAACAGGACTACTACCGTTTC
NM_001584.2         GAACTGACATTTGATAAGGAATTCATGGCAGACCTTGTTAAACAGGACTACTACCGTTTC
                    ************************************************************

HsCD00285694        CCCTCTGTGTCCAAATTGAAACCAGAGGACTTTGACAATGTTCAGTCCCTCCTGACAAAC
XM_005253110.1      CCCTCTGTGTCCAAATTGAAACCAGAGGACTTTGACAATGTTCAGTCCCTCCTGACAAAC
XM_005253112.1      CCCTCTGTGTCCAAATTGAAACCAGAGGACTTTGACAATGTTCAGTCCCTCCTGACAAAC
NM_001584.2         CCCTCTGTGTCCAAATTGAAACCAGAGGACTTTGACAATGTTCAGTCCCTCCTGACAAAC
                    ************************************************************

HsCD00285694        AGTATTTACTTACAAGATTCGGAGGTAACAGTGAAGGGATTCAGGATATACGGTGCACCT
XM_005253110.1      AGTATTTACTTACAAGATTCGGAGGTAACAGTGAAGGGATTCAGGATATACGGTGCACCT
XM_005253112.1      AGTATTTACTTACAAGATTCGGAGGTAACAGTGAAGGGATTCAGGATATACGGTGCACCT
NM_001584.2         AGTATTTACTTACAAGATTCGGAGGTAACAGTGAAGGGATTCAGGATATACGGTGCACCT
                    ************************************************************

HsCD00285694        TGGACCCCGTGGTTTAATGGATGGGGCTTTAACCTACCCAGAGGTCAGTCTCTGCTGGAC
XM_005253110.1      TGGACCCCGTGGTTTAATGGATGGGGCTTTAACCTACCCAGAGGTCAGTCTCTGCTGGAC
XM_005253112.1      TGGACCCCGTGGTTTAATGGATGGGGCTTTAACCTACCCAGAGGTCAGTCTCTGCTGGAC
NM_001584.2         TGGACCCCGTGGTTTAATGGATGGGGCTTTAACCTACCCAGAGGTCAGTCTCTGCTGGAC
                    ************************************************************

HsCD00285694        AAGTGGAACCTCATCCCTGAGGGCATTGACATACTCATGACACATGGACCTCCTCTAGGT
XM_005253110.1      AAGTGGAACCTCATCCCTGAGGGCATTGACATACTCATGACACATGGACCTCCTCTAGGT
XM_005253112.1      AAGTGGAACCTCATCCCTGAGGGCATTGACATACTCATGACACATGGACCTCCTCTAGGT
NM_001584.2         AAGTGGAACCTCATCCCTGAGGGCATTGACATACTCATGACACATGGACCTCCTCTAGGT
                    ************************************************************

HsCD00285694        TTTCGAGACTGGGTTCCAAAGGAGCTTCAAAGAGTGGGCTGTGTGGAGCTGTTAAACACG
XM_005253110.1      TTTCGAGACTGGGTTCCAAAGGAGCTTCAAAGAGTGGGCTGTGTGGAGCTGTTAAACACG
XM_005253112.1      TTTCGAGACTGGGTTCCAAAGGAGCTTCAAAGAGTGGGCTGTGTGGAGCTGTTAAACACG
NM_001584.2         TTTCGAGACTGGGTTCCAAAGGAGCTTCAAAGAGTGGGCTGTGTGGAGCTGTTAAACACG
                    ************************************************************

HsCD00285694        GTTCAGAGGCGAGTCCGGCCCAAGCTCCATGTGTTTGGTGGAATCCATGAAGGTTATGGC
XM_005253110.1      GTTCAGAGGCGAGTCCGGCCCAAGCTCCATGTGTTTGGTGGAATCCATGAAGGTTATGGC
XM_005253112.1      GTTCAGAGGCGAGTCCGGCCCAAGCTCCATGTGTTTGGTGGAATCCATGAAGGTTATGGC
NM_001584.2         GTTCAGAGGCGAGTCCGGCCCAAGCTCCATGTGTTTGGTGGAATCCATGAAGGTTATGGC
                    ************************************************************

HsCD00285694        ATCATGACCGACGGTTACACAACGTACATCAATGCCTCGACGTGTACAGTCAGCTTTCAA
XM_005253110.1      ATCATGACCGACGGTTACACAACGTACATCAATGCCTCGACGTGTACAGTCAGCTTTCAA
XM_005253112.1      ATCATGACCGACGGTTACACAACGTACATCAATGCCTCGACGTGTACAGTCAGCTTTCAA
NM_001584.2         ATCATGACCGACGGTTACACAACGTACATCAATGCCTCGACGTGTACAGTCAGCTTTCAA
                    ************************************************************

HsCD00285694        CCGACCAACCCTCCAATTATATTTGACCTTCCAAACCCACAGGGTTCCAAGGGCGAATTC
XM_005253110.1      CCGACCAACCCTCCAATTATATTTGACCTTCCAAACCCACAGGGTTCCTGA---------
XM_005253112.1      CCGACCAACCCTCCAATTATATTTGACCTTCCAAACCCACAGGGTTCCTGA---------
NM_001584.2         CCGACCAACCCTCCAATTATATTTGACCTTCCAAACCCACAGGGTTCCTGA---------
                    ************************************************:..         

HsCD00285694        GTTTAAACCTGCAGGACT
XM_005253110.1      ------------------
XM_005253112.1      ------------------
NM_001584.2         ------------------