Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
8698a1ZBt2
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCR4zeroBLUNT-TOPO
Format:
FUSION
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"Invitrogen Zero Blunt, TOPO"
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
HsCD00285702
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 666nts
Open reading frame: 51 to 716
TACGACTCACTATAGGGCGAATTGAATTTAGCGGCCGCGAATTCGCCCTTGAGGACGTGA
AGCTGGAGTTCCCTTCCCTTCCACAGTGCAAGGAAGACGCCGAGGAGTGGACCTACCCTA
TGAGACGAGAGATGCAGGAAATTTTACCTGGATTGTTCTTAGGCCCATATTCATCTGCTA
TGAAAAGCAAGCTACCTGTACTACAGAAACATGGAATAACCCATATAATATGCATACGAC
AAAATATTGAAGCAAACTTTATTAAACCAAACTTTCAGCAGTTATTTAGATATTTAGTCC
TGGATATTGCAGATAATCCAGTTGAAAATATAATACGTTTTTTCCCTATGACTAAGGAAT
TTATTGATGGGAGCTTACAAATGGGAGGAAAAGTTCTTGTGCATGGAAATGCAGGGATCT
CCAGAAGTGCAGCCTTTGTTATTGCATACATTATGGAAACATTTGGAATGAAGTACAGAG
ATGCTTTTGCTTATGTTCAAGAAAGAAGATTTTGTATTAATCCTAATGCTGGATTTGTCC
ATCAACTTCAGGAATATGAAGCCATCTACCTAGCAAAATTAACAATACAGATGATGTCAC
CACTCCAGATAGAAAGGTCATTATCTGTTCATTCTGGTACCACAGGCAGTTTGAAGAGAA
CACATGAAGAAGAGGATGATTTTGGAACCATGCAAGTGGCGACTGCACAGAATGGCAAGG
GCGAATTCGTTTAAACCTGCAGGACTAGTCCCTTTAGTGAGGGTT
Protein sequence predicted based on nucleotide sequence
EDVKLEFPSLPQCKEDAEEWTYPMRREMQEILPGLFLGPYSSAMKSKLPVLQKHGITHII CIRQNIEANFIKPNFQQLFRYLVLDIADNPVENIIRFFPMTKEFIDGSLQMGGKVLVHGN AGISRSAAFVIAYIMETFGMKYRDAFAYVQERRFCINPNAGFVHQLQEYEAIYLAKLTIQ MMSPLQIERSLSVHSGTTGSLKRTHEEEDDFGTMQVATAQNG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
666
Mutations:
None
Discrepancy:
a666c,G222G;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50 ug/mL ampicillin
Growth Condition:
Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)
Comments:
None
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-8698a
Recommended expression in:
Not Applicable
Vector Name:
pCR4zeroBLUNT-TOPO
Synonyms:
pCR-4, pCR-IV, pCR4, pCR4-TOPO
Description:
TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.
Comments:
Empty vector is a product available from Invitrogen.
Size (bp):
3956
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin of replication
3161
3834
primer site
M13 reverse
M13 reverse priming site
205
221
primer site
T3
T3 priming site
243
262
primer site
M13 forward
M13 forward priming site
355
370
primer site
T7
T7 priming site
328
347
promoter
lac
lac promoter region
2
216
selectable marker
AmpR
Ampicillin resistance gene
2203
3063
selectable marker
KanR
Kanamycin resistance gene
1159
1953
