NT5E (Homo sapiens) in pCR4zeroBLUNT-TOPO (TOPO cloning vector)

Explanation of Terms

Gene:
Gene Symbol:  NT5E
Gene Name:  5'-nucleotidase, ecto (CD73)
Original Clone ID:
8644a1ZBt1
Keyword:
intended mutation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCR4zeroBLUNT-TOPO
Format: 
FUSION
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"Invitrogen Zero Blunt, TOPO"
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HsCD00285706
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1662nts
Open reading frame: 18 to 1679

GGCGCGAATTCGCCCTTTGGCCTGCGGCTGGCGCCTGGGAGCTTACGATTTTGCACACCA
ACGACGTGCACAGCCGGCTGGAGCAGACCAGCGAGGACTCCAGCAAGTGCGTCAACGCCA
GCCGCTGCATGGGTGGCGTGGCTCGGCTCTTCACCAAGGTTCAGCAGATCCGCCGCGCCG
AACCCAACGTGCTGCTGCTGGACGCCGGCGACCAGTACCAGGGCACTATCTGGTTCACCG
TGTACAAGGGCGCCGAGGTGGCGCACTTCATGAACGCCCTGCGCTACGATGCCATGGCAC
TGGGAAATCATGAATTTGATAATGGTGTGGAAGGACTGATCGAGCCACTCCTCAAAGAGG
CCAAATTTCCAATTCTGAGTGCAAACATTAAAGCAAAGGGGCCACTAGCATCTCAAATAT
CAGGACTTTATTTGCCATATAAAGTTCTTCCTGTTGGTGATGAAGTTGTGGGAATCGTTG
GATACACTTCCAAAGAAACCCCTTTTCTCTCAAATCCAGGGACAAATTTAGTGTTTGAAG
ATGAAATCACTGCATTACAACCTGAAGTAGATAAGTTAAAAACTCTAAATGTGAACAAAA
TTATTGCACTGGGACATTCGGGTTTTGAAATGGATAAACTCATCGCTCAGAAAGTGAGGG
GTGTGGACGTCGTGGTGGGAGGACACTCCAACACATTTCTTTACACAGGCAATCCACCTT
CCAAAGAGGTGCCTGCTGGGAAGTACCCATTCATAGTCACTTCTGATGATGGGCGGAAGG
TTCCTGTAGTCCAGGCCTATGCTTTTGGCAAATACCTAGGCTATCTGAAGATCGAGTTTG
ATGAAAGAGGAAACGTCATCTCTTCCCATGGAAATCCCATTCTTCTAAACAGCAGCATTC
CTGAAGATCCAAGCATAAAAGCAGACATTAACAAATGGAGGATAAAATTGGATAATTATT
CTACCCAGGAATTAGGGAAAACAATTGTCTATCTGGATGGCTCCTCTCAATCATGCCGCT
TTAGAGAATGCAACATGGGCAACCTGATTTGTGATGCAATGATTAACAACAACCTGAGAC
ACGCGGATGAAATGTTCTGGAACCACGTATCCATGTGCATTTTAAATGGAGGTGGTATCC
GGTCGCCCATTGATGAACGCAACAATGGCACAATTACCTGGGAGAACCTGGCTGCTGTAT
TGCCCTTTGGAGGCACATTTGACCTAGTCCAGTTAAAAGGTTCCACCCTGAAGAAGGCCT
TTGAGCATAGCGTGCACCGCTACGGCCAGTCCACTGGAGAGTTCCTGCAGGTGGGCGGAA
TCCATGTGGTGTATGATCTTTCCCGAAAACCTGGAGACAGAGTAGTCAAATTAGATGTTC
TTTGCACCAAGTGTCGAGTGCCCAGTTATGACCCTCTCAAAATGGACGAGGTATATAAGG
TGATCCTCCCAAACTTCCTGGCCAATGGTGGAGATGGGTTCCAGATGATAAAAGATGAAT
TATTAAGACATGACTCTGGTGACCAAGATATCAACGTGGTTTCTACATATATCTCCAAAA
TGAAAGTAATTTATCCAGCAGTTGAAGGTCGGATCAAGTTTTCCACAGGAAGTCACTGCC
ATGGAAGCTTTTCTTTAATATTTCTTTCACTTTGGGCAGTGATCTTTGTTTTATACCAAA
AGGGCGAATTCGT

Protein sequence predicted based on nucleotide sequence

WPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLL
LDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPIL
SANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITAL
QPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPA
GKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSI
KADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMF
WNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVH
RYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNF
LANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSTGSHCHGSFSL
IFLSLWAVIFVLYQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1662
Mutations:
T376A;

Discrepancy:
g1660c,g1661a,G554Q;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50 ug/mL ampicillin
Growth Condition: Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)
Comments: None
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in NYSGXRC-8644a
Recommended expression in:
Not Applicable