Gene:
Gene Symbol:
SSH3
Gene Name:
slingshot protein phosphatase 3
Original Clone ID:
8723a1ZBt1
Keyword:
intended mutation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCR4zeroBLUNT-TOPO
Format:
FUSION
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"Invitrogen Zero Blunt, TOPO"
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
HsCD00285709
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
CGCCCTTGCCCTGGTCACAGTGAGCCGTTCGCCCCCGGGCAGCGGCGCCTCCACGCCCGT
GGGGCCCTGGGACCAGGCGGTCCAGCGAAGGAGTCGACTCCAGCGAAGGCAGAGCTTTGC
GGTGCTCCGTGGGGCTGTCCTGGGACTGCAGGATGGAGGGGACAATGATGATGCAGCAGA
GGCCAGTTCTGAGCCAACAGAGAAGGCCCCGAGTGAGGAGGAGCTCCACGGGGACCAGAC
AGACTTCGGGCAAGGATCCCAGAGTCCCCAGAAGCAGGAGGAGCAGAGGCAGCACCTGCA
CCTCATGGTACAGCTGCTGAGGCCGCAGGATGACATCCGCCTGGCAGCCCAGCTGGAGGC
ACCCCGGCCTCCCCGGCTCCGCTACCTGCTGGTAGTTTCTACACGAGAAGGAGAAGGTCT
GAGCCAGGATGAGACGGTCCTCCTGGGCGTGGATTTCCCTGACAGCAGCTCCCCCAGCTG
CACCCTGGGCCTGGTCTTGCCCCTCTGGAGTGACACCCAGGTGTACTTAGATGGAGACGG
GGGCTTCAGCGTGACGTCTGGTGGGCAAAGCCGGATCTTCAAGCCCATCTCCATCCAGAC
CATGTGGGCCACACTCCAGGTATTGCACCAAGCATGTGAGGCAGCTCTAGGCAGCGGCCT
TGTACCGGGTGGCAGTGCCCTCACCTGGGCCAGCCACTACCAGGAGAGACTGAACTCCGA
ACAGAGCTGCCTCAATGAGTGGACGGCTATGGCCGACCTGGAGTCTCTGCGGCCTCCCAG
CGCCGAGCCTGGCGGGTCCTCAGAACAGGAGCAGATGGAGCAGGCGATCCGTGCTGAGCT
GTGGAAAGTGTTGGATGTCAGTGACCTGGAGAGTGTCACTTCCAAAGAGATCCGCCAGGC
TCTGGAGCTGCGCCTGGGGCTCCCCCTCCAGCAGTACCGTGACTTCATCGACAACCAGAT
GCTGCTGCTGGTGGCACAGCGGGACCGAGCCTCCCGCATCTTCCCCCACCTCTACCTGGG
CTCAGAGTGGAACGCAGCAAACCTGGAGGAGCTGCAGAGGAACAGGGTCACCCACATCTT
GAACATGGCCCGGGAGATTGACAACTTCTACCCTGAGCGCTTCACCTACCACAATGTGCG
CCTCTGGGATGAGGAGTCGGCCCAGCTGCTGCCGCACTGGAAGGAGACGCACCGCTTCAT
TGAGGCTGCAAGAGCACAGGGCACCCACGTGCTGGTCCACTGCAAGATGGGCGTCAGCCG
CTCAGCGGCCACAGTGCTGGCCTATGCCATGAAGCAGTACGAATGCAGCCTGGAGCAGGC
CCTGCGCCACGTGCAGGAGCTCCGGCCCATCGCCCGCCCCAACCCTGGCTTCCTGCGCCA
GCTGCAGATCTACCAGGGCATCCTGACGGCCAGAACCAAGGGCGAA
Protein sequence predicted based on nucleotide sequence
ALVTVSRSPPGSGASTPVGPWDQAVQRRSRLQRRQSFAVLRGAVLGLQDGGDNDDAAEAS SEPTEKAPSEEELHGDQTDFGQGSQSPQKQEEQRQHLHLMVQLLRPQDDIRLAAQLEAPR PPRLRYLLVVSTREGEGLSQDETVLLGVDFPDSSSPSCTLGLVLPLWSDTQVYLDGDGGF SVTSGGQSRIFKPISIQTMWATLQVLHQACEAALGSGLVPGGSALTWASHYQERLNSEQS CLNEWTAMADLESLRPPSAEPGGSSEQEQMEQAIRAELWKVLDVSDLESVTSKEIRQALE LRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILNM AREIDNFYPERFTYHNVRLWDEESAQLLPHWKETHRFIEAARAQGTHVLVHCKMGVSRSA ATVLAYAMKQYECSLEQALRHVQELRPIARPNPGFLRQLQIYQGILTART
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1410
Mutations:
V58A;
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50 ug/mL ampicillin
Growth Condition:
Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)
Comments:
None
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in NYSGXRC-8723a
Recommended expression in:
Not Applicable
Vector Name:
pCR4zeroBLUNT-TOPO
Synonyms:
pCR-4, pCR-IV, pCR4, pCR4-TOPO
Description:
TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.
Comments:
Empty vector is a product available from Invitrogen.
Size (bp):
3956
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin of replication
3161
3834
primer site
M13 reverse
M13 reverse priming site
205
221
primer site
T3
T3 priming site
243
262
primer site
M13 forward
M13 forward priming site
355
370
primer site
T7
T7 priming site
328
347
promoter
lac
lac promoter region
2
216
selectable marker
AmpR
Ampicillin resistance gene
2203
3063
selectable marker
KanR
Kanamycin resistance gene
1159
1953
